RESUMO
In this paper we report on the metabolic response of human hepatocytes grown on polyethersulfone membranes surface modified with a plasma-deposited acrylic acid coating and RGD peptide covalently immobilized through a "spacer arm" molecule. The modified surfaces were characterized by means of X-ray photoelectron spectroscopy and water contact angle measurements. The performance of modified and unmodified membranes was evaluated by assessing the expression of liver specific and biotransformation functions of human hepatocytes. Diclofenac, a non-steroidal anti-inflammatory drug, was used to investigate the biotransformation functions. Surface-modified membranes elicit specific cellular responses and induce hepatocytes to enhance the synthesis rate of albumin and urea, particularly in the presence of diclofenac. Also the biotransformation functions were expressed at high levels.
Assuntos
Hepatócitos/citologia , Hepatócitos/metabolismo , Fígado Artificial , Fígado/citologia , Fígado/metabolismo , Oligopeptídeos/farmacologia , Engenharia Tecidual/métodos , Adsorção , Albuminas/biossíntese , Biotransformação , Técnicas de Cultura de Células/métodos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Hepatócitos/efeitos dos fármacos , Temperatura Alta , Humanos , Fígado/efeitos dos fármacos , Teste de Materiais , Membranas Artificiais , Oligopeptídeos/química , Ligação Proteica , Propriedades de Superfície , Ureia/metabolismoRESUMO
Continuous and modulated glow discharges were used to deposit thin films from acrylic acid vapors. Different deposition regimes were investigated, and their effect on chemical composition, morphology and homogeneity of the coatings, as well as on their stability in water and resistance to sterilization. Stable films were utilized in cell adhesion experiments with human fibroblasts.
