RESUMO
A new class of C8-linked pyrrolo[2,1-c][1,4]benzodiazepine-chalcone conjugates have been prepared by employing a solid-phase synthetic protocol. In this strategy an intramolecular aza-Wittig reductive cyclization approach has been utilized. Interestingly, some of these molecules have shown enhanced DNA-binding affinity and promising anticancer activity on a large number of human cancer cell lines.
Assuntos
Antineoplásicos/farmacologia , Benzodiazepinas/farmacologia , Linhagem Celular Tumoral/efeitos dos fármacos , Chalcona/farmacologia , DNA/metabolismo , Pirróis/farmacologia , Antineoplásicos/síntese química , Benzodiazepinas/síntese química , Sítios de Ligação , Chalcona/análogos & derivados , Chalcona/síntese química , DNA/química , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Modelos Químicos , Pirróis/síntese química , Relação Estrutura-AtividadeRESUMO
1,2,3-Triazole based molecules are useful pharmacophores for several DNA-alkylating and cross-linking agents. A series of A/C8, C/C2 and A/C8-C/C2-linked 1,2,3-triazole-PBD conjugates have been synthesized by employing 'click' chemistry. These molecules have exhibited promising DNA-binding affinity and anticancer activity in selected human cancer cell lines.
Assuntos
Antineoplásicos/síntese química , Benzodiazepinas/síntese química , DNA/química , Pirróis/síntese química , Triazóis/síntese química , Antineoplásicos/química , Antineoplásicos/farmacologia , Benzodiazepinas/química , Benzodiazepinas/farmacologia , Linhagem Celular Tumoral , DNA/metabolismo , Humanos , Cinética , Neoplasias/tratamento farmacológico , Neoplasias/genética , Pirróis/química , Pirróis/farmacologia , Triazóis/química , Triazóis/farmacologiaRESUMO
A versatile method for the solid-phase synthesis of imidazo[1,2-a]pyridine-based derivatives, imidazo[1,2-a]pyridine-8-carboxamides, has been developed. They were obtained by treatment of the amino group of the polymer-bound 2-aminonicotinate with different alpha-haloketones, followed by halogenation at the 3-position of the polymer-bound imidazo[1,2-a]pyridine. The derived polymer-bound imidazo[1,2-a]pyridines 5, 6, and 7 were finally cleaved from the solid-support with an excess of primary or secondary amines. The final crude products were purified from excess amines by solid-supported liquid-liquid extraction (SLE).
Assuntos
Amidas/síntese química , Piridinas/química , Amidas/química , Espectroscopia de Ressonância Magnética , Espectrometria de Massas por Ionização por Electrospray , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
A versatile combinatorial approach was developed and utilized for the rapid synthesis of pyrrolo[2,1-c]-[1,4]benzodiazepine-5,11-dione (PBD-5,11-dione) libraries 10, 15, and 19 containing 210 compounds with varied substitutions in A, B, and C rings. The key aspect of the synthetic strategy includes Staudinger, intermolecular aza-Wittig reaction followed by imine reduction and base-mediated cyclative cleavage results in the formation of final resin-free compounds. This strategy provides a highly efficient and practical protocol for the parallel synthesis of PBD-5,11-diones on solid support. The modifications in the C-ring of the PBD scaffold produced three types of sublibraries. Reactions were monitored by FT-IR spectroscopy on the resin beads. Further, from a generated library of 210 compounds, 142 compounds have been selected and evaluated for in vitro activity against Mycobacterium tuberculosis, and some of these compounds have exhibited promising activity.
Assuntos
Antituberculosos/síntese química , Benzodiazepinonas/síntese química , Benzodiazepinonas/farmacologia , Pirróis/síntese química , Pirróis/farmacologia , Antituberculosos/farmacologia , Técnicas de Química Combinatória/métodos , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Mycobacterium tuberculosis/efeitos dos fármacos , Prolina/química , Espectrometria de Massas por Ionização por Electrospray , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
Benzodiazepine based heterocycles can be prepared efficiently on solid-support by employing different approaches. In this review, an effort has been made to highlight academic and industrial examples of combinatorial synthesis for this type of heterocycles published in the last decade. Therefore, it describes synthetic strategies for the generation of benzodiazepine privileged structures employing the corresponding resin-bound substrates. Further, the most relevant biological properties of these heterocycles have also been incorporated.
Assuntos
Benzodiazepinas/síntese química , Técnicas de Química Combinatória/métodos , Alcaloides/síntese química , Benzodiazepinas/química , Benzodiazepinonas/síntese química , Benzodiazepinonas/química , Estrutura Molecular , Pirróis/química , Triazóis/síntese químicaRESUMO
Quinoxaline, quinazoline and benzimidazole based templates have been synthesized on solid-support employing different methodologies. This review enlightens academic and industrial examples of combinatorial synthesis for this type of heterocycles that appeared in the literature in the last decade. Hence, some of the important synthetic strategies for the generation of quinoxaline, quinazoline and benzimidazole based privileged structures, and the important biological activities for these heterocycles have been highlighted. Further, benzothiadiazinone, thioxoquinazolinone, cinnoline and indazole are also examined in this review.
Assuntos
Benzimidazóis/síntese química , Técnicas de Química Combinatória/métodos , Quinazolinas/síntese química , Quinoxalinas/síntese química , Benzimidazóis/química , Benzotiadiazinas/síntese química , Compostos Heterocíclicos com 2 Anéis/química , Indazóis/síntese química , Estrutura Molecular , Ftalazinas/síntese química , Piranos/síntese químicaRESUMO
Fluoroquinolones have been synthesized and linked to DC-81 at C8 position through different alkyl chain spacers. These PBD conjugates have exhibited good DNA binding affinity, and a representative compound shows promising in vitro anticancer activity.