Epstein-Barr virus positive inflammatory pseudo-tumour of the spleen: A case report and literature review.

INTRODUCTION: Epstein-Barr virus positive inflammatory pseudo-tumour (IPT) of the spleen is an uncommon, frequently asymptomatic entity, which is typically picked up as an incidental finding on imaging. PRESENTATION OF CASE: We present a case of EBV positive IPT of the spleen which presented as an incidental finding on CT in a patient with a history of malignancy. Splenectomy was performed. DISCUSSION: IPTs are benign spindle cell lesions of varying aetiology, which can arise in a variety of tissues, including the spleen. In situ hybridisation showed strong staining for Epstein-Barr virus RNA in our case, in common with many similar lesions described in the literature. The differential diagnosis of such spindle cell tumours is discussed. CONCLUSION: Radiologically, EBV positive spindle cell tumours are indistinguishable from malignant lesions such as lymphoma and diagnosis is made on histology, usually at splenectomy.

Highest genetic diversity at the northern range limit of the rare orchid Isotria medeoloides.

Populations in previously glaciated regions are often genetically depauperate in comparison with populations at lower latitudes, due either to bottlenecks experienced in post-glacial colonization or to contemporary genetic drift in small, peripheral populations. Populations of the rare self-fertilizing North American orchid Isotria medeoloides are largest in the previously glaciated region near the northern range limit, allowing us to examine the role of historical versus contemporary processes in determining population genetic diversity and structure. If contemporary processes predominate, genetic diversity should increase with increasing census size. In contrast, if sequential bottlenecks associated with colonization are paramount, diversity should decrease with latitude and be relatively insensitive to census size. We genotyped 299 individuals from 20 populations at four variable microsatellite loci to contrast genetic diversity and structure for populations in previously glaciated regions versus previously unglaciated regions. Populations were highly inbred (F=0.95) and highly differentiated (R(ST)=0.485). Across all sampled populations, genetic diversity decreased and genetic differentiation increased with declining population size. Small southern populations were especially differentiated and genetically depauperate. In the glaciated part of the range, genetic diversity increased as populations approached the northern range limit, demonstrating the centrality of contemporary processes for this post-glacial colonist.

Synthesis and magnetic properties of Fe3O4 nanoparticles coated with biocompatible double hydrophilic block copolymer.

Fe3O4 nanoparticles coated with double hydrophilic biocompatible poly(sodium(2-sulfamate-3-carboxylate)isoprene)-b-poly(ethylene oxide) block copolymer were prepared by a one step precipitation method. The magnetic nanoparticles have 15 nm mean diameter (TEM), 68 nm hydrodynamic diameter, -30.10 mV zeta-potential and form very stable dispersion in aqueous media. Structural characterization using powder XRD and Mössbauer spectroscopy establish the magnetite phase, while thermogravimetric analysis and FT-IR spectroscopy confirm the presence of the block copolymer on the nanoparticles surface. The magnetic properties were determined using a vibrating sample magnetometer (VSM) at room temperature and reveal superparamagnetic behavior while the composite materials shows high saturation magnetization up to 67.7 emu/gr.

Factors that affect the food choices made by girls and young women, from minority ethnic groups, living in the UK.

BACKGROUND: Lower birth weight, often found in infants from minority ethnic groups, may be partly because of the disproportionate representation of ethnic minority groups in low-income areas. To develop an intervention, to improve the nutritional intake of young women from populations at risk of low-birth-weight babies, which would be culturally sensitive and well received by the intended recipients, a community development approach was used to investigate factors that might influence food choice and the nutritional intake of girls and young women from ethnic minority groups. METHODS: Focus group discussions were conducted across the UK, to explore factors that might affect the food choices of girls and young women of African and South Asian decent. The data was analysed using deductive content analysis (Qual. Soc. Res., 1, 2000, 1). Discussions were around the broad themes of buying and preparing food, eating food and dietary changes, and ideas for an intervention to improve diet. RESULTS: The focus group discussions indicated that all the communities took time, price, health and availability into consideration when making food purchases. The groups were also quite similar in their use of 'Western' foods which tended to be of the fast food variety. These foods were used when there was not enough time to prepare a 'traditional' meal. CONCLUSION: Many issues that affect the food choice of people who move to the UK are common within different ethnic groups. The idea of a practical intervention based on improving cooking skills was popular with all the groups.

Prevalence and attributes of roll-your-own smokers in the International Tobacco Control (ITC) Four Country Survey.

BACKGROUND: Roll-your-own (RYO) cigarettes are often substantially less expensive than factory made (FM) cigarettes, and appear to be increasing in popularity-perhaps because smokers seek out less expensive options to maintain their nicotine addiction. There is surprisingly little research available on the actual prevalence of RYO cigarette usage, and even less on the attributes of those who smoke RYO cigarettes. OBJECTIVES: This study has two objectives: (1) to compare the prevalence of RYO versus FM cigarette usage among adult smokers in Australia, Canada, the United Kingdom, and the United States; and (2) to compare the attributes of exclusive FM smokers, exclusive RYO smokers, and those who report "mixed" RYO and FM use. DESIGN: The data were collected from the International Tobacco Control (ITC) Four Country Survey (ITC-4), a random digit dialed telephone survey of representative samples of over 9046 adult smokers from the following four countries: Australia (n = 2301), Canada (n = 2,206), the UK (n = 2400), and the USA (n = 2,139), surveyed between October and December 2002, and on 6075 smokers followed-up, on average, seven months later. RESULTS: The prevalence of RYO cigarette usage varied widely across the four countries, with a low of 6.7% in the USA, to 28.4% in the UK. Exclusive use of RYO cigarettes was more common in the UK than in the other three countries. The use of RYO cigarettes was associated with having a lower annual income, male sex, younger average age, higher level of nicotine addiction, a stronger belief that RYO tobacco is less harmful compared to other forms of tobacco, and a more positive perception of tobacco use. Prevalence of RYO use was relatively stable within each of the four countries between the baseline and follow-up survey. RYO use was unrelated to quitting activity at follow-up, although mixed RYO users who had made a quit attempt were more likely to relapse than either exclusive FM or exclusive RYO smokers. CONCLUSIONS: Patterns of RYO use vary considerably across Australia, Canada, the USA, and the UK. RYO smokers are a heterogeneous group; however, the factors associated with RYO use appear to be the same across the four countries studied.

The effect of Mn doping in FePt nanoparticles on the magnetic properties of the L1(0) phase.

FePtMn nanoparticles with a narrow size distribution and an average diameter of 3 nm were synthesized by the chemical reduction of Fe(acac)(3) and Pt(acac)(2) by NaBH(4) and the thermal decomposition of Mn(2)(CO)(10) in phenyl ether. The as-made nanoparticles have a disordered face-centred cubic (fcc) structure, which transformed after thermal treatment at 650 °C to an ordered face-centred tetragonal (fct) structure, possessing coercivity values up to 13.7 kOe at room temperature. The coercivity of the annealed samples depends on the amount of Mn added to the reaction mixture, with the coercive field increasing significantly with the partial substitution of Pt by Mn, while the partial substitution of Fe by Mn does not affect the magnetic properties strongly.

Pramwalking as postnatal exercise and support: an evaluation of the Stroll Your Way To Well-Being program and supporting resources in terms of individual participation rates and community group formation.

Stroll Your Way To Well Being is a community pramwalking program designed to increase access by mothers to sociable postnatal exercise. This study evaluated the program in terms of long-term adherence rates by mothers to walking, and the effectiveness of kits distributed to providers in assisting them to set up their own groups. Approximately one quarter of information kit recipients established a pramwalking group. Reasons for non-initiation included lack of time or a perceived lack of priority. Mothers reported program benefits to be the opportunity to exercise, socialize and share information about baby issues. 70% of mothers were still walking 16 months after the program's commencement. Reasons for mothers ceasing participation in pramwalking included lack of interest, no group leader available or their baby was too old. Future studies need to focus on the prevention and treatment of postnatal depression through exercise.

The effects of thiopentone and propofol on delayed hypersensitivity reactions.

The effects of thiopentone and propofol on delayed hypersensitivity reactions and T lymphocyte proliferation were studied in nine healthy volunteers (five women and four men). Thiopentone 5 mg.kg-1 and propofol 2.5 mg kg-1 were given as a 10 min infusion on two separate occasions. The volunteers were exposed to a skin multitest antigen before and after administration of the two agents and their skin reactions assessed. T lymphocyte responses were studied using phytohaemagglutinin (PHA)-induced proliferation. Results showed that both drugs caused a significant depression of skin reactions in vivo but no depression in the T lymphocyte proliferation.

Effect of four i.v. induction agents on T-lymphocyte proliferations to PHA in vitro.

Anaesthetic agents are believed to have an adverse effect on human immunity. We have studied the effects of four i.v. induction agents, thiopentone, methohexitone, etomidate and propofol, on T-lymphocyte proliferations to phytohaemagglutinin in vitro. We found that at plasma concentrations similar to those obtained after induction doses, all drugs, with the exception of propofol, caused depression of T-lymphocyte function.

Epidural analgesia for labour using a continuous infusion of bupivacaine and alfentanil.

Seventy women who requested epidural pain relief in labour received a continuous epidural infusion at 8 ml h-1, which contained either 0.25% bupivacaine (n = 29), or a mixture of 0.125% bupivacaine and 0.005% alfentanil (n = 31), assigned randomly. Increments of 4 ml 0.25% bupivacaine were given on demand to prevent residual pain from uterine contractions. The two groups were compared for motor block of the lower limbs, number of increments required, type of delivery and neonatal Apgar scores. The group of women receiving the mixture of bupivacaine and alfentanil required between them seven increments; the group receiving bupivacaine alone required 15. One of the women receiving the mixture and eight of the women receiving bupivacaine alone had almost complete motor block. There were no differences in the mode of delivery or in the neonatal Apgar scores.

An outbreak of gastro-intestinal illness caused by eggs containing Salmonella enteritidis phage type 4.

Seven of nine people developed gastro-intestinal symptoms between 18 and 27 h after eating a trifle with a topping containing raw eggs. Salmonella enteritidis Phage Type (PT) 4 was isolated both from the faeces of affected persons and from eggs remaining in the same box as those used to prepare the topping. The findings show that eggs being produced and sold in the U.K. still contain S. enteritidis PT4 and that this is continuing to cause human infection.