Study on epidemiology of cutaneous amyloidosis in northern India and effectiveness of dimethylsulphoxide in cutaneous amyloidosis.

CONTEXT: Amyloidosis, which is characterized by the extracellular deposition of a proteinaceous substance, is usually associated with considerable tissue dysfunction. However, the etiology of the disease remains uncertain and the treatment disappointing. AIM: 1. To know the epidemiology of cutaneous amyloidosis 2. To evaluate the effect of dimethylsulphoxide on cutaneous amyloidosis. SETTINGS AND DESIGN: Data was collected from patients attending the Outpatient Department (OPD) over a period of one year. MATERIAL AND METHODS: Patients were screened on the basis of signs and symptoms and then confirmed histologically. A total of 62 patients who were suspected to be suffering from amyloidosis on the basis of clinical signs and symptoms and 38 patients who were further confirmed histopathologically underwent the treatment. STATISTICAL ANALYSIS USED: Chi-square test was used for testing the significance of proportions. RESULTS: 63.15 percent of the patients had macular amyloidosis and the interscapular area was the most common area involved (52.63%). Pruritus, pigmentation, and papules responded excellently to dimethylsulphoxide after one month of treatment. CONCLUSIONS: Cutaneous amyloidosis is a disease found in middle-aged persons, with a female preponderance, and dimethylsulphoxide seems to be an effective therapy.

Study of gene probes in childhood leprosy.

The objective was to detect nucleic acids of M. leprae in skin lesions of leprosy patients and study the effect of treatment on these nucleic acids, using r-RNA gene probes, using a cross sectional study. The study was carried out at Department of Paediatrics, S.N. Medical College, Agra and Department of Microbiology, Central JALMA Institute for leprosy, Agra. The study included 32 cases of leprosy less than 16 years of age, divided into 3 groups viz. without treatment (12 cases), in middle of treatment (11 cases) and at the end of treatment (9 cases). All cases were subjected to a detailed history and thorough clinical examination. All of them had smear examination and lepromin test done and their skin biopsies were subjected to gene detection. Nucleic acids were isolated from skin biopsies of all cases by standard procedure. After dot blotting of these nucleic acids, they were hybridised with radioactive (p32) r-RNA probes. The results were interpreted after getting the X-ray films processed with background signals from controls. Majority of cases were between 13-16 years of age. As age advanced, the disease moved from tuberculoid end of spectrum towards lepromatous end (p < 0.05). Majority of paucibacillary (PB) cases were lepromin positive while majority of multibacillary (MB) cases were lepromin negative (p < 0.05). In specimens of untreated cases, 50% of PB specimens and 87.5% of MB specimens were positive for r-RNA probes. In multibacillary type 100% smear positive specimens and 67% smear negative specimens were positive for r-RNA probes. In patients during the middle of treatment positivity for r-RNA decreased and 20% of PB specimens and 16.6% MB specimens were positive. At the end of treatment (1 year for PB cases and 2 years for MB cases), the results of r-RNA were negative, which indicated that the treatment regimens used in the study were effective. This study supports the usefulness of r-RNA probes as a diagnostic and therapeutic tool in childhood leprosy.

Creeping Eruption At an Unusual Site.

A 9 year old female child developed creeping eruption on the neck which is an unusual site.

Cutaneous Haemangioma Associated with Nasal Deformity.

A case of strawberry haemangioma with destruction of nasal septum resulting in nasal deformity is reported.

A Study of Blastogenic Transformation Under Phytomaemagglutinin (pha) in Leprosy.

The present study was aimed to assess the cell-mediated immune response under PHA stimulus in leprosy patients and healthy contacts. Twenty-one cases suffering from leprosy were included in the study. They were diagnosed clinically by skin biopsy,. smear examination and lepromin reaction. Five cases had lepromatous, 7 borderlines and 9 tuberculoid leprosy. Eleven were healthy contacts. Lepromin reaction was negative in 5 patients in lepromatous group while it was positive in the other patients and healthy contacts. Blastogenic transformation was depressed only in patients with lepromatous leprosy. In tuberculoid and borderline cases, lymphocytes exhibited similar reactivity with PHA as was shown by the controls. Healthy contacts of lepromatous leprosy patients were leprom'm positive and did not show any C M I depression. it is suggested that depression of PHA response may develop after the on set of leprosy infection.

Studies on the Antifungal Activity of Pterocarpus Marsupium: a Clinical Evaluation.

Pmarsupium (Hindi Bijasar) is a powerful astringent and is used chiefly in diarrhoeas. It is also an useful remedy for diabetes mellitus and various skin diseases as mentioned in literature. In a blind clinical trial, the usefulness of this drug as a topical agent against T.cruris and T. corporis was elevated. The drug yielded good response within 3 days of the first application.

Contact dermatitis caused by copper sulfate used as coloring material in commercial alcohol.

Copper sulfate added to commercial alcohol as a coloring agent caused contact dermatitis of the hands in 10 furniture polishers. Patch tests with 5% aqueous solution of copper sulfate were positive in all these patients but negative in 15 normal controls.