RESUMO
A simple, precise, accurate, and sensitive RP-HPLC method for simultaneous determination of cefixime trihydrate and dicloxacillin sodium in combined tablet dosage form was developed and validated. Chromatographic separation of the two drugs was performed on a Purospher BDS C18 column (25 cm x 4.6 mm id, 5 microm particle size). The mobile phase methanol-0.01 M phosphate buffer (75 + 25, v/v), adjusted to pH 3 with glacial acetic acid, was delivered at a flow rate of 1.0 mL/min. Detection was performed at 227 nm. Separation was completed within 10 min. Calibration curves were linear with R2 between 0.99 to 1.0 over a concentration range of 2-10 microg/mL for cefixime trihydrate and 5-25 micromL for dicloxacillin sodium. The RSD for intraday and interday precision was < 2.0%.
Assuntos
Cefixima/análise , Química Farmacêutica/métodos , Cromatografia Líquida de Alta Pressão/métodos , Dicloxacilina/análise , Tecnologia Farmacêutica/métodos , Soluções Tampão , Calibragem , Cromatografia/métodos , Formas de Dosagem , Relação Dose-Resposta a Droga , Concentração de Íons de Hidrogênio , Tamanho da Partícula , Fosfatos/química , Reprodutibilidade dos Testes , ComprimidosRESUMO
Two accurate, precise, rapid and economical methods viz. Absorption correction method and Dual wavelength method were developed for the estimation of Cefixime (CEF) and Erdosteine (ERDO) in capsule dosage form. In both the methods linearity was observed in the concentration range of 2-25 microg/ml for Cefixime and 3-37.5 microg/ml for Erdosteine. The results of the analysis have been validated statistically and by recovery studies. The percentage assay was found to be 100.03 +/- 0.68 for Cefixime and 99.5 +/- 1.0 for Erdosteine (Mean +/- S.D) by method A and 99.54 +/- 0.84 for Cefixime and 100.54 +/- 1.3 for Erdosteine (Mean +/- S.D) by method B respectively.
