British Orthodontic Society, Chapman Prize Winner 2003. A novel in vitro culture model to investigate the reaction of the dentine-pulp complex to orthodontic force.

OBJECTIVE: To develop a novel mandible slice organ culture model to investigate the effects of externally applied force on the dentine-pulp complex. DESIGN: In vitro organ culture. SETTING: School of Dentistry, Birmingham, UK. MATERIALS AND METHODS: Transverse 2 mm thick sections were cut from the mandibles of five 28-day-old male Wistar rats. Serial sections were used for control and test pairs. Springs made from 0.016-inch and 0.019 x 0.025-inch stainless steel wires were used to apply a 50 g tensile or compressive force, respectively, to test specimens. Control and test specimens were cultured for 5 days in a humidified incubator with 5% CO(2) at 37 degrees C and processed for routine histological investigation. Nine more rats were used to provide control and compression test pairs where the pulps were extirpated after 3 days culture and total RNA isolated for gene expression analysis by reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: Histology showed the dental and supporting tissues maintained a healthy appearance in the control cultures after culture. Histomorphometric analysis revealed a 20-27% increase in pulp fibroblast density in test specimens compared with controls. Gene expression analyses revealed up-regulation in the test groups of PCNA, c-Myc, Collagen 1alpha, TGF-beta1 and alkaline phosphatase, whilst expression of osteocalcin was reduced. CONCLUSIONS: The results demonstrated that the present organ culture technique provides a valuable in vitro experimental model for studying the effects of externally applied forces. These forces stimulated a cellular response in the pulp chamber characterized by altered gene expression and proliferation of fibroblasts; the latter being unaffected by the nature of the force in terms of compression or tension.

A randomized clinical trial comparing the accuracy of direct versus indirect bracket placement.

OBJECTIVE: To determine the accuracy of direct or indirect bracket placement. DESIGN: A prospective, randomized comparison of 2 different methods of bracket placement. SETTING: Queens Hospital, Burton upon Trent, UK between February and May 2001. MATERIALS AND METHOD: Twenty-six consecutive patients requiring upper and lower MBT trade mark pre-adjusted Edgewise appliances had their labial segments bonded directly or indirectly according to a split mouth system of allocation. Before and after bond-up all brackets were photographed and measured from tracings to determine positional differences from the ideal. RESULTS: Using ANOVA (General Linear Model), vertical errors were found to be greater than those in the horizontal plane, which in turn were greater than angular errors (p<0.05). Errors were greater in the maxillary arch than in the mandibular arch. There was no significant difference between the mean errors produced by the two methods of bracket placement. CONCLUSIONS: Mean bracket placement errors were similar with both techniques.

The Burton approach to indirect bonding.

There has been a resurgence of interest in the indirect bonding technique for orthodontics. The procedure is not often used in the UK and the reasons for this, as well as the renewal of interest, are explored. A costing exercise suggests that the cost-effectiveness of the technique may be improved by the described Burton technique and, in the light of current manpower considerations, the implications for its use in the future are also discussed.