RESUMO
AIMS: The objective of our study was to analyze the potential prognostic value of the expression of the serine protease HtrA1 and of EGFR in 70 malignant mesotheliomas. MATERIALS & METHODS: Immunohistochemistry was used to determine the expression of HtrA1 and EGFR. Univariate and multivariate analyses were used to correlate expression of these molecular factors in combination with available clinicopathologic data to patient survival. RESULTS: A positive, statistically significant relationship has been recorded between HtrA1 expression level and survival (p < 0.0001). By contrast, a negative relationship has been identified between EGFR expression and survival (p = 0.02). Moreover, extension of the tumor (T) and involvement of lymph nodes (N) advanced status (p = 0.001 and 0.002, respectively), as well as the sarcomatoid histotype (p = 0.005), correlated significantly with poor survival. Finally, by a multivariate Cox regression analysis, the only immunohistochemical parameter that resulted to influence overall survival was HtrA1 (p = 0.0001). Interestingly, the prognostic value of HtrA1 expression was completely independent from EGFR expression (p < 0.0001). CONCLUSION: This is the first study of the relationship between HtrA1 expression and survival of mesothelioma patients. The data obtained strongly indicate the utilization of HtrA1 expression as a prognostic parameter for mesothelioma and suggest this serine protease as a possible molecular target for the treatment of malignant mesotheliomas.
Assuntos
Mesotelioma/diagnóstico , Mesotelioma/enzimologia , Serina Endopeptidases , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/química , Biomarcadores Tumorais/metabolismo , Linhagem Celular Tumoral , Receptores ErbB/biossíntese , Feminino , Seguimentos , Serina Peptidase 1 de Requerimento de Alta Temperatura A , Humanos , Imuno-Histoquímica , Masculino , Mesotelioma/mortalidade , Mesotelioma/patologia , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos , Serina Endopeptidases/biossíntese , SobrevidaRESUMO
Primary melanoma of the gastrointestinal tract is a rare, highly malignant neoplasm that is associated with an unfavorable long-term prognosis. Animal studies are needed to further characterize this tumor and to develop new and more effective protocols. A spontaneous canine intestinal melanoma is described which, because of its local aggressive behavior and advanced stage, was not treatable with conventional strategies, thus being a potential candidate for investigational trials.
Assuntos
Abdome/diagnóstico por imagem , Doenças do Cão/diagnóstico , Neoplasias Intestinais/veterinária , Melanoma/veterinária , Animais , Biópsia , Doenças do Cão/diagnóstico por imagem , Doenças do Cão/patologia , Cães , Endossonografia/veterinária , Neoplasias Intestinais/diagnóstico , Neoplasias Intestinais/diagnóstico por imagem , Neoplasias Intestinais/patologia , Masculino , Melanoma/diagnóstico , Melanoma/patologia , Estadiamento de Neoplasias/veterinária , PrognósticoRESUMO
Cell adhesion is a basic count in inter- and intracellular communication and plays an important role in tumor progression. In this study, the expression of E-selectin, ICAM-1 and VCAM-1 were evaluated by means of immunohistochemistry in a group of 153 lung cancer specimens. E-selectin immunoreactivity was localized mostly on endothelial cell venules and capillaries with an average staining intensity of 75% of cells in the NSCLC, while in SCLC the intensity of the staining was 69%. The staining pattern for ICAM-1 reached an average intensity of 57%, in both NSCLC and SCLC. Finally, VCAM-1 immunoreactivity was detected only in NSCLC with an average intensity of 12% on endothelial cell venules and capillaries. This study provides a contribution towards the understanding of the basic mechanisms of cell adhesion in lung cancer progression.
