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1.
Talanta ; 190: 30-37, 2018 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-30172514

RESUMO

A very sensitive electrochemical biosensor to determine totals triglycerides (TGs) in serum samples has been developed. It is based on the electrochemical oxidation of glycerol at glassy carbon electrodes modified with magnetic nanoparticles bonded to lipase enzyme and copper oxide nanoparticles, both supported on a multiwalled carbon nanotubes/pectin dispersion. Glycerol is produced by enzymatic reaction between the TGs present in samples and the lipase immobilized. The quantification of triglycerides was performed by amperometric measurements. The proposed electrochemical biosensor improves the performance of others methods developed for the TGs quantification. The determination of TGs does not need a pretreatment of serum samples. The PLS-1 algorithm was used for the quantification of TGs. According to this algorithm, the of detection and quantification limits were from 3.2 × 10-3 g L-1 to 3.6 × 10-3 g L-1, and from 9.6 × 10-3 to 1.1 × 10-2 g L-1, respectively. The sensitivity was 1.64 × 10-6 A L g-1. The proposed electrochemical biosensor exhibited a very good performance, a stability of 20 days, very good reproducibility and repeatability, and it is presented as a very good alternative for the determination of TGs in human serum clinical samples.


Assuntos
Técnicas Biossensoriais/métodos , Lipase/metabolismo , Nanocompostos/química , Triglicerídeos/sangue , Calibragem , Quitosana/química , Cobre/química , Eletroquímica , Eletrodos , Enzimas Imobilizadas/química , Enzimas Imobilizadas/metabolismo , Humanos , Lipase/química , Nanopartículas de Magnetita/química , Nanotubos de Carbono/química , Pectinas/química , Triglicerídeos/química
2.
G Ital Dermatol Venereol ; 147(1): 71-81, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22370570

RESUMO

AIM: Atopic dermatitis (AD) is a common, chronic relapsing inflammatory skin disease characterized by dry skin and variable pruritus sometimes associated with allergic disease in other organs as asthma and rhinoconjunctivitis. AD affects deeply the Quality of Life, thus can be extremely disabling and may cause psychological problems for both affected children and their families. METHODS: In order to investigate the estimated prevalence of the disease and the beliefs of the Italian pediatricians, a group of 437 Italian family pediatricians covering a population of almost 380000 children participated in a study based on a questionnaire of 38 items. RESULTS: According to answers of the participants, the incidence of AD has been estimated around 10% of the population and food allergy is believed to be the trigger of the acute phase of the disease in infants. As a second opinion, dermatologists are consulted more frequently than allergologists. CONCLUSION: The use of emollients is advised in general whilst topical corticosteroids treatment is prescribed only in selected cases; more than 50% of pediatricians do not prescribe topical calcineurin inhibitors.


Assuntos
Atitude do Pessoal de Saúde , Dermatite Atópica/tratamento farmacológico , Criança , Dermatite Atópica/epidemiologia , Feminino , Hospitais Pediátricos , Humanos , Masculino , Pediatria , Prevalência , Inquéritos e Questionários
3.
Boll Ist Sieroter Milan ; 62(1): 91-5, 1983 Mar 31.
Artigo em Italiano | MEDLINE | ID: mdl-6639774

RESUMO

A statical investigation conducted by the authors in a random population demonstrated the utility of the determination of antitetanic antibodies by means of Tetan test in order to establish the immunological defence level against infection.


Assuntos
Anticorpos Antibacterianos/análise , Tétano/imunologia , Adolescente , Adulto , Idoso , Vacinas Bacterianas/imunologia , Feminino , Testes de Hemaglutinação , Humanos , Imunidade , Masculino , Pessoa de Meia-Idade
4.
Boll Ist Sieroter Milan ; 62(1): 96-9, 1983 Mar 31.
Artigo em Italiano | MEDLINE | ID: mdl-6639775

RESUMO

Authors investigated tetanic immunity in pediatric age by means of titration of antitetanic antibodies in serum.


Assuntos
Anticorpos Antibacterianos/análise , Tétano/imunologia , Criança , Pré-Escolar , Feminino , Humanos , Imunidade , Masculino
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