RESUMO
OBJECTIVE: To improve the correct rate of ABO genotyping by meliorating AS-PCR primer. METHODS: The primer P1 was changed into primer P1' by substituting the fifth base G for C of 3' end and the ABO genotyping results of primer P1 and P1' was compared and analysed. RESULTS: The non-specific product of OO typing is reducing and the wrong genotyping of OO and AO was avoided by meliorating AS-PCR primer. CONCLUSION: The rates of wrong ABO genotyping results could be effectively reduced by using altering primer P1'.
Assuntos
Sistema ABO de Grupos Sanguíneos/genética , Alelos , Primers do DNA/genética , Reação em Cadeia da Polimerase/métodos , Sequência de Bases , Genótipo , Humanos , Dados de Sequência Molecular , Sensibilidade e EspecificidadeRESUMO
OBJECTIVE: To identify sarcosaphagous flies and their larvae, pupa. METHODS: Sarcosaphagous flies and their larvae, pupas were collected from human corpses and their surroundings in the Weifang city. A 304 bp region in COI gene was analyzed by mtDNA sequencing. RESULTS: The studied region showed no sequence divergence within same species and significant difference were found between different species in all samples. CONCLUSION: It is a practical approach to identify these Sarcosaphagous flies and their larvae, pupas by sequence analysis of the 304bp region of the COI in mtDNA.
