Analysis of the Pattern, Alliance and Risk of rs1799752 (ACE I/D Polymorphism) with Essential Hypertension.

Studies in spontaneously hypertensive rat had revealed an elevated level of ACE gene expression in the tissues and is substantiated by experimental clinical studies for a positive correlation between ACE I/D polymorphism and hypertension. Aim: To determine whether the polymorphic variant of ACE gene in intron 16 confers susceptibility to essential hypertension. I/D polymorphism at the locus intron 16 of the ACE gene were amplified from the genomic DNA of the total 571 (hypertensive patients, n: 279; controls, n: 292) participants using polymerase chain reaction and gel electrophoresis methods and were examined in a case-control approach. Suitable descriptive statistics was used for different variables. Result revealed significant heterogeneity under the allele (p: 0.0002) and genotype (p: 0.0001) contrast in hypertensive patients than in normal controls, with an increased frequency of D allele (62.72%; p < 0.0001; OR: 1.8144; 95% CI: 1.4327-2.2979) and DD genotype (41.93%; p: < 0.0001). A significant association was found in the DD variant with disease phenotype (p: 0.0018, 95% CI: 1.3303-3.4907; OR: 2.1549; Table 31) and is substantiated by the data of multivariate analysis, demonstrating a statistically significant increase in odds of hypertension with the ACE D/D genotype (OR: 2.09; 95% CI: 1.24-2.91). Conspicuously, subgroup analysis by gender did not change this pattern of results. Albeit the allele distribution resulted in a higher frequency of the D/D genotype in the cases than controls, testing genetic equilibrium between the observed and expected genotypes using Hardy-Weinberg equilibrium showed ACE gene variants were confirming to the law in hypertensive as well as in non-hypertensive participants. I/D polymorphism in the angiotensin-I-converting enzyme gene at the 16th intron can be useful for outcome predictions during diagnostic processes can be implicated in an individual's propensity for hypertension and thus implies that genetic variants of ACE I/D might serve as a predictor for the susceptibility to hypertension.

Association of Mbo I-RFLP at the Renin Locus (rs2368564) with Essential Hypertension.

Several lines of experimental and clinical evidence have alluded a pivotal role of renin in blood pressure homeostasis and therefore a relevance of molecular variants of the renin gene and essential hypertension have been speculated. This study was designed to evaluate the pattern, alliance and risk of renin Mbo I (10631A>G; rs2368564) polymorphism at the locus intron 9 for a possible role in modulating essential hypertension in adult population from Gujarat (India). A total of 257 consecutively enrolled essential hypertensive patients and 270 controls were genotyped using polymerase chain reaction-restriction fragment length polymorphism method for the selected marker. Suitable descriptive statistics was used for different variables. Genotypic (x(2) 10.43, p 0.0054) and allelic (x(2) 11.46, p 0.0007) distribution of this SNP displayed significant differences between cases and controls with an increased frequency of the A allele (x(2) 6.275; p 0.0122) and A/A geno-type (x(2) 8.247; p 0.0041) in hypertensive individuals. However, it showed no deviation from Hardy-Weinberg equilibrium in either affected or control group. A significant association was found in the A/A variant of rs2368564 with essential hypertension (p 0.0032), along with a statistically significant increase in odds of hypertension (OR 1.69; CI 1.46-2.28; p 0.02), even after confounding factors were adjusted in multiple logistic regression analysis and is substantiated by inter-genotypic variations in the mean systolic and diastolic blood pressure in patients. In conclusion, renin 10631A>G gene mutation at the ninth intron play critical roles in BP (dys)regulation and can be implicated in an individual's susceptibility for hypertension.

Effect of Raja yoga meditation on the lipid profile of post-menopausal women.

BACKGROUND: Coronary artery disease is an important cause of death and disability among older women. Modification in lipid profile lowers the risk of coronary artery disease. It is claimed that yoga and transcendental meditation have a cholesterol lowering effect. This study was designed to assess the effect of raja yoga meditation of Brahmakumaris which is very simple to practice, on serum lipids in normal Indian women. METHODS AND RESULTS: 49 normal female volunteers were the subjects. They were divided into pre-menopausal (n=23) and post-menopausal (n=26) groups. They were further divided into non-meditators (who had never done any kind of meditation), short-term meditators (meditating for 6 months to 5 years) and long-term meditators (meditating for more than 5 years). Lipid profile was assessed using their respective reagent sets. Serum cholesterol, triglyceride and low-density lipoprotein-cholesterol in nonmeditators were significantly more in post-menopausal women as compared to pre-menopausal women. Serum cholesterol and low density lipoprotein-cholesterol were significantly lowered in both short and long term meditators as compared to non-meditators in post-menopausal women. No significant difference was observed in lipid profile in pre-menopausal women. CONCLUSION: Raja yoga meditation lowered serum cholesterol and low-density lipoprotein-cholesterol in post-menopausal women thus reducing the risk of coronary artery disease in them.

Effect of meditation on respiratory system, cardiovascular system and lipid profile.

In this study, respiratory functions, cardiovascular parameters and lipid profile of those practicing Raja Yoga meditation (short and long term meditators) were compared with those of nonmediators. Vital capacity, tidal volume and breath holding were significantly higher in short and long term meditators than nonmeditators. Long term mediators had significantly higher vital capacity and expiratory pressure than short term meditators. Diastolic blood pressure was significantly lower in both short and long term meditators as compared to nonmeditators. Heart rate was significantly lower in long term meditators than in short term meditators and nonmeditators. Lipid profile showed a significant lowering of serum cholesterol in short and long term meditators as compared to nonmeditators. Lipid profile of short and long term meditators was better than the profile of nonmeditators inspite of similar physical activity. This shows that Raja Yoga meditation provides significant improvements in respiratory functions, cardiovascular parameters and lipid profile.