Biotechnology to reduce logistics burden and promote environmental stewardship for Air Force civil engineering requirements.

This review provides discussion of advances in biotechnology with specific application to civil engineering requirements for airfield and airbase operations. The broad objectives are soil stabilization, waste management, and environmental protection. The biotechnology focal areas address (1) treatment of soil and sand by biomineralization and biopolymer addition, (2) reduction of solid organic waste by anaerobic digestion, (3) application of microbes and higher plants for biological processing of contaminated wastewater, and (4) use of indigenous materials for airbase construction and repair. The consideration of these methods in military operating scenarios, including austere environments, involves comparison with conventional techniques. All four focal areas potentially reduce logistics burden, increase environmental sustainability, and may provide energy source, or energy-neutral practices that benefit military operations.

Effect of anaerobic digester inoculum preservation via lyophilization on methane recovery.

A robust anaerobic digestion (AD) inoculum is key to a successful digestion process by providing the abundant bacteria needed for converting substrate to useable methane (CH4). While transporting digester contents from one AD to another for digester startup has been the norm, transportation costs are high, and it is not feasible to transport wet inoculum to remote locations. In this study, the impact of preservation of AD inoculum via lyophilization was investigated for the purposes of digester startup and restabilization. The effect of lyophilizing inoculum on CH4 production using food waste as the substrate was tested using biochemical methane potential (BMP) tests under the following conditions: (1) three inoculum sources, (2) two inoculum to substrate ratios (ISR), (3) two cryoprotectants, and (4) two inoculum growth phases. After lyophilization with skim milk, the three inocula produced 144-146 mL CH4/g volatile solids (VS) and 194-225 mL CH4/g VS at a 2:1 and 4:1 ISR, respectively, with 33-57% more CH4 at the 4:1 ISR. Preservation with 10% skim milk exhibited complete recovery of CH4 production, while 10% glycerol and 10% glycerol/skim milk mixture yielded 76% and 4% CH4 recovery, respectively. Inoculum growth phase before preservation (mid-exponential or stationary growth phase) did not significantly affect CH4 recovery. The study indicates that inoculum can be preserved via lyophilization using 10% skim milk as a cryoprotectant and reactivated for food waste digestion. The results provide a systematic quantification of the conditions needed to successfully preserve a mixed AD inoculum.

Reverse osmosis processing of organic model compounds and fermentation broths.

Post-treatment of an anaerobic fermentation broth was evaluated using a 150 gal/day, single cartridge prototype reverse osmosis (RO) system. Baseline tests were conducted at 25 degrees C using six organic model compounds representing key species found in the fermentation broth: ethanol, butanol, acetic acid, oxalic acid, lactic acid, and butyric acid. Correlations of the rejection and recovery efficiencies for these organic species, individually and in simulated mixtures, were obtained as a function of feed pressure with and without recirculation of the retentate. The actual fermentation broth obtained from a continuous-flow biohydrogen process was treated by the RO system under the operating conditions similar to those used in the baseline tests, resulting in greater than 95% removal of total organic carbon. These results are encouraging and useful for further studies on the feasibility of incorporating the RO technology into an integrated and field deployable wastewater management and water recovery system.