Application of Blurred Image Processing and IoT Action Recognition in Sports Dance Sports Training.

In order to process the blurred image, this study proposes to combine the blurred point functions in the invariant space into multiple blurred images and then restore them through the deconvolution operation. The PSF functions of the fuzzy invariant space are combined to obtain the fuzzy invariant space. Finally, a gradual restoration method is used to perform many blurred image processing steps. The experimental results prove that the method proposed in this study can avoid the noise introduced in the process of multiple deconvolutions, can reduce the calculation error, and can improve the recovery effect. Based on fuzzy image processing, this research studies the nature of human motion and the identification of actions in the Internet of Things, which provides new ideas and methods for recognition research. The Kinect somatosensory camera of the Internet of Things is used to capture deep images, and 20 three-dimensional points of the human skeleton structure are obtained through its SDK. Based on this, the motion characteristics of human joints were studied, and a motion resolution model suitable for the Internet of Things was proposed. The model has low complexity, simple calculation, and sorting characteristics. Based on this, this research study also uses software engineering ideas and general methods of system development to design and create sports dance management information systems and uses advanced methods such as computers and the Internet to maintain training management to achieve optimal sports training for sports dance mode and to provide information about the management of sports dance athletes training to improve efficiency and the management level.

Functional expression of TWEAK and the receptor Fn14 in human malignant ovarian tumors: possible implication for ovarian tumor intervention.

The aim of this current study was to investigate the expression of the tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) and its receptor fibroblast growth factor-inducible 14 (Fn14) in human malignant ovarian tumors, and test TWEAK's potential role on tumor progression in cell models in-vitro. Using immunohistochemistry (IHC), we found that TWEAK and its receptor Fn14 were expressed in human malignant ovarian tumors, but not in normal ovarian tissues or in borderline/benign epithelial ovarian tumors. High levels of TWEAK expression was detected in the majority of malignant tumors (36 out of 41, 87.80%). Similarly, 35 out of 41 (85.37%) malignant ovarian tumors were Fn14 positive. In these malignant ovarian tumors, however, TWEAK/Fn14 expression was not corrected with patients' clinical subtype/stages or pathological features. In vitro, we demonstrated that TWEAK only inhibited ovarian cancer HO-8910PM cell proliferation in combination with tumor necrosis factor-α (TNF-α), whereas either TWEAK or TNF-α alone didn't affect HO-8910PM cell growth. TWEAK promoted TNF-α production in cultured THP-1 macrophages. Meanwhile, conditioned media from TWEAK-activated macrophages inhibited cultured HO-8910PM cell proliferation and invasion. Further, TWEAK increased monocyte chemoattractant protein-1 (MCP-1) production in cultured HO-8910PM cells to possibly recruit macrophages. Our results suggest that TWEAK/Fn14, by activating macrophages, could be ovarian tumor suppressors. The unique expression of TWEAK/Fn14 in malignant tumors indicates that it might be detected as a malignant ovarian tumor marker.

The relationship between plasma and placental tissue factor, and tissue factor pathway inhibitors in severe pre-eclampsia patients.

OBJECTIVE: To investigate the mechanism underlying the hypercoagulable state in severe pre-eclampsia. METHODS: Plasma tissue factor (TF) and tissue factor pathway inhibitor (TFPI) expression from pre-eclampsia patients and healthy pregnant controls were determined by ELISA. Placental TF and TFPI gene and protein expression were detected by quantitative RT-PCR, immunohistochemistry, and Western analysis. RESULTS: The plasma TF level in the pre-eclampsia group was significantly higher than the control group (p<0.01), and surprisingly, the plasma TFPI-1 and TFPI-2 in the pre-eclampsia group were significantly lower (p<0.01). Placental TF gene and protein expression levels in the pre-eclampsia group was significantly higher than the control group, while TFPI-1 and TFPI-2 levels were significantly lower (p<0.05). Lastly, a significant correlation was found between plasma and placental TF protein levels in the pre-eclampsia group (p<0.01). CONCLUSION: Higher expression and/or release of TF from the placenta may contribute towards a pathological hypercoagulable state in pre-eclampsia patients.

Arsenic trioxide and cisplatin synergism increase cytotoxicity in human ovarian cancer cells: therapeutic potential for ovarian cancer.

Drug resistance is a major concern in the successful treatment of ovarian cancer. In the present study we report a combinational drug regime using arsenic trioxide (ATO) and cisplatin (CDDP) to increase therapeutic potentiality in ovarian cancer cells. ATO-mediated growth inhibition and apoptosis in human suspension ovarian cancer COC1 cells were evaluated by MTT assay and annexin V assay using flow cytometry, respectively. cDNA arrays were performed to screen ATO-mediated gene expression. Treatment of COC1 cells with ATO alone resulted in growth inhibition and apoptosis with a dose-and time-dependent fashion; further cDNA arrays showed that 34 genes (23 up-regulated genes and 11 down-regulated genes) may strongly associate with the antiproliferative and pro-apoptotic effects induced by ATO. Furthermore, Chou-Talalay analysis was used to evaluate the combinational effect of ATO and CDDP as well as dose-reduction index (DRI) in a panel of ovarian cancer cells including CDDP-sensitive and -resistant cell lines. The combination index (CI) analysis indicated that the interaction effect of ATO/CDDP exhibited a wide range of synergism in all the adherent ovarian cancer cells (A2780, IGROV-1, SKOV-3, and R182) as well as 0.93 to 0.69 for IC(50) to IC(90) in suspension COC1 cells where CI < 1, =1, and >1, define synergism, additive effect, and antagonism, respectively. More intriguingly, the combination of ATO and CDDP yielded favorable DRIs ranging from 1.23-fold to 13.51-fold dose reduction. These results suggest that ATO and its combination with CDDP present therapeutic potential for ovarian cancer, and deserve further preclinical and clinical studies.

TWEAK promotes ovarian cancer cell metastasis via NF-kappaB pathway activation and VEGF expression.

The poor prognosis of human ovarian cancer is partly due to its metastasis and recurrence. It has been demonstrated that tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK)-fibroblast growth factor inducible-14 (Fn14) signaling system may be a potential regulator of human tumorigenesis. The objective of this study was to understand the effect of TWEAK on ovarian cancer metastasis. We recently showed that activation of Fn14 signaling by TWEAK promoted cell migration and invasion in human HO-8910PM cells. Treating HO-8910PM cells with TWEAK resulted in the activation of nuclear factor-kappa B (NF-kappaB) and subsequently the translocation of NF-kappaB from cytoplasm to nucleus. In addition, TWEAK promoted vascular endothelial growth factor (VEGF) protein expression, and this effect was dependent upon NF-kappaB transcriptional activity. Blocking the NF-kappaB pathway with PDTC suppressed TWEAK-induced up-regulation of VEGF protein expression and cell metastasis. Our results suggest that TWEAK-Fn14 functions, in part, through the NF-kappaB signaling pathway to up-regulate VEGF expression to foster ovarian cancer cell metastasis. Targeted therapy against TWEAK-Fn14 signaling system as an adjuvant to surgery may improve clinical management of invasive ovarian cancer cells and advance the outcome of this devastating cancer.

Coagulation and fibrinolysis related cytokine imbalance in preeclampsia: the role of placental trophoblasts.

OBJECTIVE: Cytokine imbalance might have a pivotal role in hypercoagulability seen in preeclampsia. Our objective was to determine the relationship of blood coagulation related factors in placental tissue and peripheral blood in preeclamptic and normal pregnancies. METHODS: We compared mRNA and protein levels of tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor-1 (PAI-1), and tissue factor (TF) in the placenta of normal and preeclamptic pregnancies. Placental and peripheral blood t-PA and PAI-1 levels were examined. Trophoblasts were used to study the effects of hypoxia, hypoxia-reperfusion, and inflammatory cytokines on t-PA, PAI-1, tissue factor pathway inhibitor (TFPI), and TF. RESULTS: PAI-1 and TF mRNA and protein levels were higher in placental tissue of preeclamptic pregnancies and in the peripheral blood of patients with preeclampsia. mRNA and protein secretion of TF, TFPI, PAI-1, but not t-PA, was increased in trophoblast cell culture under hypoxia and hypoxia-reoxygenation. Cell cultures with high levels of tumor necrosis factor-alpha (TNF-alpha) exhibited increased expression and secretion of TF and PAI-1, decreased TFPI, and no significant change of t-PA. CONCLUSIONS: Imbalanced synthesis of t-PA, PAI-1, TFPI, and TF in trophoblasts may contribute to hypercoagulability in patients with preeclampsia.

The role of TSP-1 on decidual macrophages involved in the susceptibility to unexplained recurrent spontaneous abortion.

PROBLEM: To investigate the role of TSP-1 on decidual macrophages (DMPhi) in unexplained recurrent spontaneous abortion (RSA). METHOD OF STUDY: A total of 20 women undergoing artificial abortion in the first trimester and 10 patients with RSA were selected. (i) The expression of TSP-1 mRNA in deciduas was detected by real-time polymerase chain reaction; (ii) Flow cytometry was used to detect the percentage of positive TSP-1, CD36, CD47 markers on macrophages. (iii) Cytokine expression was measured by enzyme-linked immunosorbent spot-forming (ELISPOT) cell assay. RESULTS: (i) The expression of TSP-1 mRNA on DMPhi in RSA was significantly decreased (P < 0.05). (ii) The increased expression of CD36 (P < 0.01) and the decreased expression of TSP1 (P < 0.01) were found on DMPhi of RSA. No different CD47 expression level on DMPhi was observed between two groups. (iii)The expression of IL-10 in DMPhi of RSA patients was decreased significantly compared with that of controls (P < 0.05). When adding TSP1 into culture medium, there was no change in IFN-gamma expression, but increased IL-10 (P < 0.05) expression in DMPhi of RSA patients was observed. The expression of IL-10 was decreased significantly in DMPhi from controls when adding anti-TSP-1 antibody to culture medium (P < 0.05). CONCLUSION: TSP-1 on DMPhi could influence IL-10 expression as Th2 cytokines. The abnormal expression of TSP-1 could make some women undergo pregnancy loss.

[Expression of lethal gene mRNA on placenta villi in patients with spontaneous abortion].

OBJECTIVE: To study the role of lethal gene: including retinoic X receptor (RXR)alpha, N-myc and transcript enhancer factor (TEF)-1 in human spontaneous abortion. METHODS: The levels of RXRalpha, N-myc and TEF-1 mRNA expression on placenta villi from 38 spontaneous abortion women and 33 normal early pregnant women were examined by reverse transcription, polymerase chain reaction. RESULTS: (1) The levels of RXRalpha mRNA on placenta villi from the abortion group were significantly decreased as compared with those from normal group (0.4 +/- 0.3 versus 0.6 +/- 0.3, P < 0.05); There were no significant differences in the levels of N-myc mRNA expression between the abortion group and normal group (2.1 +/- 1.2 versus 2.2 +/- 0.9, P > 0.05). The levels of TEF-1 mRNA on placenta from abortion group were significantly lower than those from normal group (1.6 +/- 1.1, 2.3 +/- 1.2, P < 0.05); (2) The levels of RXR alpha mRNA, TEF-1 mRNA in recurrent abortion group were significantly lower than those from non-recurrent group (0.3 +/- 0.2 versus 0.6 +/- 0.4, P < 0.05, 1.0 +/- 1.1 versus 1.9 +/- 1.2, P < 0.05). CONCLUSION: It was suggested that the lower expression of RXR alpha mRNA, TEF-1 mRNA may play an important role in the pathogenesis of spontaneous abortion especially in recurrent abortion.