[Predictive value of fasting plasma glucose and body mass index of pre-pregnancy women for gestational diabetes mellitus].

OBJECTIVE: To analyze the relationship between fasting plasma glucose (FPG) and body mass index (BMI) of pre-pregnancy women and the occurrence of gestational diabetes mellitus(GDM), and to explore a effective method for GDM screening. METHODS: This was a retrospective clinical study including the pregnant women who underwent screening for GDM recently and also participated in national free preconception health examination at three medical centers. They were divided into normal group and GDM group base on the 2011 diagnosis criteria for GDM of our country. RESULTS: FPG, BMI and diastolic blood pressure before pregnancy in GDM group were significantly higher than those in normal group (P<0.01). Multiple logistic-regression showed that the women with higher FPG and BMI level before pregnancy had higher risk of diagnosis of GDM. The receiver operating characteristic (ROC) curves showed that the area under the curve (AUC) were 0.600 and 0.632 for FPG and BMI, respectively, in predicting GDM (P<0.01). CONCLUSION: The FPG and BMI level of women before pregnancy are valuable predictors for early diagnosis of GDM.

Quantitative determination of tilmicosin in canine serum by high performance liquid chromatography-tandem mass spectrometry.

A highly sensitive and quantitative LC/MS/MS assay for the determination of tilmicosin in serum has been developed and validated. For sample preparation, 0.2 mL of canine serum was extracted with 3 mL of methyl tert-butyl ether. The organic layer was transferred to a new vessel and dried under nitrogen. The sample was then reconstituted for analysis by high performance liquid chromatography-tandem mass spectrometry. A Phenomenex Luna C8(2) analytical column was used for the chromatographic separation. The eluent was subsequently introduced to the mass spectrometer by electrospray ionization. A single range was validated for 50-5000 ng/mL for support of toxicokinetic studies. The inter-day relative error (inaccuracy) for the LLOQ samples ranged from -5.5% to 0.3%. The inter-day relative standard deviations (imprecision) at the respective LLOQ levels were < or =10.1%.