An odorant receptor mediates the attractiveness of cis-jasmone to Campoletis chlorideae, the endoparasitoid of Helicoverpa armigera.

Parasitic wasps rely on olfaction to locate their hosts in complex chemical environments. Odorant receptors (ORs) function together with well-conserved odorant coreceptors (ORcos) to determine the sensitivity and specificity of olfactory reception. Campoletis chlorideae (Hymenoptera: Ichneunmonidae) is a solitary larval endoparasitoid of the cotton bollworm, Helicoverpa armigera, and some other noctuid species. To understand the molecular basis of C. chlorideae's olfactory reception, we sequenced the transcriptome of adult male and female heads (including antennae) and identified 211 OR transcripts, with 95 being putatively full length. The tissue expression profiles, as assessed by reverse-transcription PCR, showed that seven ORs were expressed only or more highly in female antennae. Their functions were analysed using the Xenopu slaevis oocyte expression system and two-electrode voltage-clamp recordings. CchlOR62 was tuned to cis-jasmone, which was attractive to female C. chlorideae adults and H. armigera larvae in the subsequent behavioural assays. Further bioassays using caged plants showed that the parasitism rate of H. armigera larvae by C. chlorideae on cis-jasmone-treated tobacco plants was higher than on the control plants. Thus, cis-jasmone appears to be an important infochemical involved in the interactions of plants, H. armigera and C. chlorideae, and CchlOR62 mediates the attractiveness of cis-jasmone to C. chlorideae.

Film cooling performance on curved walls with compound angle hole configuration.

In order to explore the effect of compound angle holes on film cooling over a convex wall and a concave wall, the present study adopts the transient liquid crystal thermography for conducting the film cooling measurement on simple hole and expanded-hole configurations. Two compound angles of 0 and 45 deg are tested at an elevated mainstream turbulence condition (Tu) of 3.8%. The test pieces have the different radius of curvature (2r/D) of 92.5 on convex and 86.5 on concave, and the same pitch to diameter ratio (P/D) of 3 on both convex and concave walls. All measurements were conducted under the mainstream Reynolds number (Re(d)) of 1700 on convex and 2300 on concave with the density ratio between coolant and mainstream (rho c/rho m) of 0.98. In current study, the effect of blowing ratio (M) on film cooling performance is investigated by varying the range of blowing ratio from 0.5 and 2.0. The present measured results show that the forward-expanded hole injection provides better surface protection than the simple hole injection. As far as the injection angle is concerned, compound angle injection provides higher film effectiveness than simple angle injection. However, the forward-expanded hole in injection (beta = 0 degree) has the best performance on both convex and concave surfaces.