In vitro study on cytotoxicity and intracellular formaldehyde concentration changes after exposure to formaldehyde and its derivatives.

HeLa cells were exposed to formaldehyde and its metabolic derivatives, methanol, formic acid, and acetaldehyde, to investigate that the toxicity of formaldehyde is not caused by the chemical group. After 1 h of treatment with formaldehyde, mitochondrial assays showed that low concentrations (e.g. 10 µmol/L) of formaldehyde promoted growth of the HeLa cells, while higher concentrations (e.g. ≥62.5 µmol/L) inhibited cell growth; while all four chemicals at a concentration of 125 µmol/L affected cell growth, formaldehyde affected the largest. Reactive oxygen species concentration increased with the concentration of the exposure chemical. The endogenous formaldehyde content increased the most in the formaldehyde group, but in other three groups, it did not increase as the exposure concentration increased. Expression of dehydrogenase (formaldehyde dehydrogenase (FDH)) in the formaldehyde (10.40) and methanol (10.60) groups increased significantly compared with the control (1), while it was similar to the control in formic acid (0.90) and acetaldehyde (1.10) groups. Our results suggest that formaldehyde could affect cell activity and even enter cells. Exposure to formaldehyde changes the endogenous formaldehyde concentration in cells within 24 h, and this induces expression of FDH for formaldehyde degradation to maintain the formaldehyde balance. The toxicity of formaldehyde is not caused by the carbon atoms in the aldehyde, hydroxyl, or carboxyl groups. Formaldehyde is hypothesized to be an important signaling molecule in the regulation of cell growth and maintenance of the endogenous formaldehyde level.

Acid volatile sulfides and simultaneously extracted metals in a metal-polluted area of Taihu Lake, China.

There were similar distribution characteristics for acid volatile sulfides (AVS) and simultaneously extracted metals (SEM) in surface sediments, and the concentrations of AVS and SEM decreased from the deposition area to the center of the bay (lake). The ratio of AVS to SEM was <1 in the surface sediments, indicating that heavy metals in surface sediments may be bioavailable. The concentration of AVS increased with sediment depth, followed by a decrease with large variation, while the concentration of SEM remained constant. By comparing the concentration of SEM with total metals, it was shown that extracted Cu and Ni decreased with sediment depth, indicating increasing association of Cu and Ni with sulfides in deeper sediment layers. The lower extracted ratios for Pb and Zn compared with sulfidic sediment illustrated that AVS should not have strong control on sediment Pb and Zn. The molar ratio of AVS and reactive iron showed that heavy metals were dynamic and active in sediments in this lake.

Effects of exogenous organic chelators on phytochelatins production and its relationship with cadmium toxicity in wheat (Triticum aestivum L.) under cadmium stress.

Phytochelatins (PCs) have been proposed as a potential biomarker for metal toxicity. In this study, cadmium (Cd) toxicity, PCs production and their relationship in wheat under Cd stress were examined using various exogenous organic chelator-buffered nutrient solutions. Single Cd stress produced strong toxic effects, as indicated by decreases of growth parameters, high level of lipid peroxidation in leaf and overproduction of PCs in root. Exogenous organic chelators with proper dose more or less reduced Cd toxicity by increasing growth parameters and decreasing lipid peroxidation in leaves. Of organic chelators (EDTA, DTPA, citric acid, malic acid and oxalic acid), EDTA was the most effective in decreasing Cd toxicity in plants, followed by DTPA and citric acid. Simultaneously, the concentrations of Cd-induced PCs in roots decreased, and the greatest decrease was caused by application of EDTA and DTPA. Linearly positive relationships were observed between Cd toxicity and root PCs concentrations under the influences of organic chelators, particularly EDTA, DTPA and citric acid. Furthermore, present results provide stronger evidence that PCs synthesis in plant cells was related to free Cd ion concentrations, not total Cd, and demonstrate that the levels of PCs production in plants correlated well with toxic effects caused by the bioavailable Cd levels.

Effects of interactions between cadmium and zinc on phytochelatin and glutathione production in wheat (Triticum aestivum L.).

It has been proposed that phytochelatins (PCs) act as a biomarker for the evaluation of metal toxicity. Little attention has been paid to the effects on metal combinations and glutathione (GSH), the most abundant cellular thiol. In the present study the effects of interactions between cadmium (Cd) and zinc (Zn) on PC and GSH production were examined in wheat tissue over 14 days' exposure. The results showed that the presence of Zn alleviated Cd toxicity, accompanied by a reduction of Cd uptake. Cd and Zn exposure increased PC-SH levels in concentration-, tissue- and time-dependent manners. Of the two metals, Cd was more effective than Zn in PC-SH production. Interactions of Cd and Zn with respect to PC-SH production may be synergistic or inhibitory, strongly depending on duration of exposure and concentration of the metal combinations. Cd also stimulated GSH production in concentration-, tissue- and time-dependent manners, whereas Zn had no significant effects on GSH levels. Compared to the presence of Cd alone, the presence of Zn reduced GSH levels in a tissue-dependent manner over the growth period. The results of the study suggest that metal interactions should be highly considered in the application of PCs and GSH as potential biomarkers for the evaluation of metal toxicity, as most metal-polluted natural environments are contaminated with more than one metal.