[QTL analysis for rice stripe disease resistance gene using recombinant inbred lines (RILs) derived from crossing of Kinmaze and DV85].

Rice stripe disease transmitted by small brown planthopper (Laodelphax striatellus Fall.) is one of the most serious viral diseases in East Asia. The disease is severely epidemic in most rice growing areas where the main cultivars are susceptible or moderately susceptible to rice stripe virus. In this research, a recombinant inbred lines (RILs) population of 81 lines derived from a cross of Kinmaze (japonica)/DV85(indica) by the single seed descent method was used to detect quantitative trait loci (QTL) conferring resistance to rice stripe virus(RSV). The response of the two parents and 81 RILs to RSV were investigated by inoculating seedlings with viruliferous small brown planthopper insects, and scored by the disease rate index. The quantitative trait loci for rice stripe disease resistance were analyzed by QTL Cartographer software. Three QTL controlling RSV resistance were detected on chromosomes 1, 7 and 11, respectively. Individual QTL accounted for 19.8%-30.9% of the phenotypic variance in the RILs population. The direction of the additive gene effects at two loci qStv7 and qStv11 coincided with that predicted by phenotypes of the parents. At these two loci, the DV85 alleles increased the resistance to RSV, while at qStv1, the Kinmaze alleles increased the resistance to RSV.

[Clinical analysis of 45 patients with severe acute respiratory syndrome].

OBJECTIVE: To explore the clinical and radiology features of Severe Acute Respiratory Syndrome (SARS). METHODS: Analysis the clinical presentations, chest radiographs, courses of disease and the features of severe pneumonia of 45 cases with SARS diagnosed between April 5 and April 20, 2003 in our hospital. RESULTS: SARS appears to has high infectivity, and cause of the outbreak. The most common symptom is fever (in 100 percent of the patients). The count of leukocyte is normal or decrease (leukopenia). 80 percent of SARS patients became fever for 24 hours and then have abnormal chest radiographs, serial chest radiographs showed progression of pulmonary infiltrates within 48 hours in 71.1 percent of patients. The percent of severe pneumonia (24.4%) is higher than typical pneumonia. CONCLUSION: SARS is a disease with high infectivity, and has its own clinical and radiology features. Early recognition, prompt isolation, and appropriate therapy are the key in combating this infection.

Clinical analysis of 45 patients with severe acute respiratory syndrome.

OBJECTIVE: To explore the clinical and radiological features of severe acute respiratory syndrome (SARS). METHODS: Analysis of the clinical presentation, chest radiographs, course of disease and features of severe pneumonia in 45 SARS patients diagnosed at our hospital in Beijing between April 5 - 20, 2003. Also included is a summation of the clinical features of SARS. RESULTS: (1) SARS appears to have high infectivity; (2) the most common symptom is fever; (3) the count of leukocyte is normal or decreased; (4) most patients (35/45, 77.8%) had experienced a 24-hour fever prior to the abnormal chest X-ray changes which showed progression of pulmonary infiltrates within 48 hours in 71.1% (32/45) of the patients and, (5) the percentage of patients who developed severe pneumonia (24.4%) is higher than those who developed typical pneumonia. CONCLUSION: SARS is a disease with high infectivity and has its own clinical and radiological features. Early recognition, prompt isolation, and appropriate therapy are the key to combat this infection.

[Clinical investigation of outbreak of nosocomial severe acute respiratory syndrome].

OBJECTIVE: To summarize and analyze the clinical manifestation, diagnosis and therapeutic strategy of severe acute respiratory syndrome (SARS), and its characteristics of outbreak in hospital via infection transmission cluster associated with SARS patients. METHODS: (1) Two hospitalized community acquired SARS cases with their clinical data and epidemiologically linked transmission clusters in our hospital were analyzed; (2) The clinical data from 41 SARS cases were summarized. RESULTS: (1) The contagiousness of SARS virus in SARS patients was extremely strong, especially in their acute stage. (2) The majority of clinical manifestations were fever (100% cases), and there were no obvious respiratory symptoms in more than half of the patients (53.7%) at early SARS stage (day 1 to day 3), accompanied by leucopenia(52.6%) and lymphopenia (68.4%). (3) Timely administration of Ribavirin, Methylprednisolone, immunoenhancer (such as interferon alpha-2b, thymosin-alpha1) to SARS patients was efficacious to certain extent in controlling the development of the disease. CONCLUSION: It is very important for clinicians to pay great attention to protect themselves from infection of SARS. Early discovery of SARS, early isolation of SARS patients, and early treatment are very helpful to improving patient's prognosis and controlling SARS overspread.