Clinical and epidemiological features of high-risk human papillomavirus infection in patients with cervical intraepithelial lesions.

OBJECTIVE: In this study, we analyzed the clinical and epidemiological features of high-risk human papillomavirus (HR-HPV) infection in patients with cervical intraepithelial lesions. METHODS: Retrospective analysis was performed on the clinical data of 240 cases of histologically confirmed cervical squamous intraepithelial lesions to determine any correlation between HPV infection characteristics, age distribution, and cervical epithelial lesions. RESULTS: Patients between the ages of 31 and 40 with cervical intraepithelial lesions were more likely to have high-grade squamous intraepithelial lesions (HSIL; 40.7%) than low-grade squamous intraepithelial lesions (LSIL; 31.3%) (P < 0.05). In patients with HSIL, HR-HPV16, HR-HPV33, and HR-HPV52 were the most common types of HPV infection, while in patients with LSIL, HR-HPV16, HR-HPV52, and HR-HPV58 were the most common types of HPV infection. The highest percentage of single infections occurred in the HSIL group (69.6%), followed by the LSIL group (68.8%). HSIL was present in a significant number of patients (28.6%) aged 30 years and above who tested positive for 12 HPV types but negative for TCT. CONCLUSION: The prevalence of HSIL is greatest in younger patients. Patients with cervical epithelial lesions typically have a single infection of a high-risk HPV genotype-HR-HPV16, HR-HPV33, HR-HPV52, or HR-HPV58. Patients aged 30 years and above who test positive for one of 12 types of HPV but negative for TCT are at increased risk for developing HSIL. In order to detect cervical lesions early and begin treatment without delay, colposcopy should be performed regardless of whether or not a high-risk HPV infection is present.

[Effects of Sediment Microenvironment on Sedimentary Phosphorus Release Under Capping].

The sediment microenvironment has an important effect on the release of endogenous phosphorus. In this study, the influence of two different in-situ covering materials on the sediment microenvironment were compared, and the controlling effect of endogenous phosphorus release were studied. The sediment microenvironment was represented by the concentration of NH4+-N, Fe2+ in interstitial water, and microbial activity. The results showed that the concentrations of NH4+-N and Fe2+ were lower under ACPM coverage than those of the Phoslock® group, and the microbial activity was higher, indicating that the redox potential of ACPM coverage was higher than that of Phoslock®. Furthermore, the bottom sediment microenvironment was in an aerobic state. Compared with the Phoslock® group, the DIP concentrations in both the overcover water and porewater in the ACPM group were higher, indicating that Phoslock® was superior to ACPM with respect to the phosphate adsorption capacity, suggesting that the sediment microenvironment was not the only criterion for phosphorus adsorption. In the fixation process of endogenous phosphorus, both of the covering materials were conducive to the fixation of inter phosphorus, and Ca-P increased significantly. On the other hand, oxidizing ACPM led to an increase in NH4Cl-P and a significant decrease in Fe/Al-P. This promotes the release of active phosphorus, which is conducive to the cleaning of the sediment and phosphorus release.

[Phosphate Control Effect and Water Body Remediation of Magnesium Modified Reed Biochar].

Adding biochar from harvested reed to sediments is a new method to control the release of sedimentary phosphorus. Three types of Mg-modified biochars were prepared by pyrolysis of reed modified by magnesium chloride at 300, 450, and 600℃.The phosphate adsorption characteristics of the three types of biochars were analyzed by isothermal adsorption experiments. Biochar MBC-450 with good phosphate adsorption effects was selected as the material for the following experiments. Taking the sediment and overlying water of a campus river as the experimental object, we studied the adsorption of phosphate in overlying water and the control of sedimentary phosphorus by Mg-modified biochar under different dosage modes (mixing and covering). The concentration of DIP in the overlying water could be effectively reduced by biochar mixing and covering, and the accumulative phosphorus adsorption capacity increased by 17.3% (mixing) and 11.7% (covering) compared with that of the control. The control effect of sedimentary phosphorus by biochar mixing was more obvious; the DIP in sedimentary water decreased by 14.7%, 18.9%, and 35.36% from 0-2 cm to 4-6 cm compared with the control. The DIP in sedimentary water decreased by 33.3%, -28.2%, and 12.9% when covered with biochar. Compared with the control, the proportion of NH4 Cl-P in the sediment of 0-2 cm and 2-4 cm increased by 15% and 15% (mixing) and 12% and 2% (covering), respectively, while BD-P in TP decreased by 7% and 9% (mixing) and 6% and 3% (covering), respectively, and the Al-P in TP decreased by 7% and 6% (mixing) and 7% and -1% (covering), respectively. The other forms of phosphorus did not change significantly. Biochar mixing and covering can both improve the microbial activity in surface sediment, and biochar mixing can improve the microbial activity in deeper sediments more significantly.

[Adsorption Properties of Sludge-hydrochar for Methylene Blue].

A low-cost and efficient adsorbent, based on sewage sludge, was prepared by hydrothermal carbonization. The sludge-hydrochar was prepared at different temperatures (160, 190, 220, and 250℃) and different reaction times (1, 4, 8, and 16 h). It was applied to the adsorption of methylene blue (MB) in water. This study analyzed the structure and physicochemical properties of hydrochar by BET, FT-IR, and zero charge points. In addition, we investigated the optimum hydrothermal conditions for the preparation of adsorbents and adsorption properties for MB by experiments. Studies have shown that 190℃ and 4 h were the best hydrothermal conditions for the preparation of adsorbent (SS190-4). SS190-4 has the largest specific surface area (11.916 m2·g-1) and the best removal rate (96.44%) for methylene blue (MB). The adsorption of MB is more favorable when the solution is alkaline. The adsorption conforms to the Langmuir isotherm equation, and the maximum model adsorption capacity for MB is 400 mg·g-1. When the concentration of hydrochar is 0.5 g·L-1, it is more economical and reasonable. When there are coexisting ions in the solution, the adsorption capacity of hydrochar to MB is inhibited. The adsorption process of MB by hydrochar conforms to the quasi-secondary kinetic model, which is a spontaneous exothermic reaction.

[Effect of Calcium Peroxide Composite Tablets on Water Remediation and Phosphorus Control in Sediment].

Two types of calcium peroxide composite tablets (CPCTs) were prepared, and the inhibition effect on the release of endogenous phosphorus and the influence on the overlying water by mixed-dosing were investigated. The CPCTs were made of calcium peroxide (CaO2), calcined water purification sludge, and hydroxypropyl methylcellulose (HPMC), which were directly pressed into composite tablets, among which Tablet-B (T-B) contained ferrous sulfate (FeSO4) and Tablet-A (T-A) did not. Both the tablets agreed well with the Langmuir and Freundlich isothermal models; the theoretical maximum adsorption capacities of T-A and T-B on phosphorus were 110.908 mg·g-1 and 106.390 mg·g-1, respectively. Compared with the control group, the pH of overlying water was increased, the concentration of Chl-a was decreased by 42.75% and 60.82%, and the DO was increased by 53.73% and 63.30% in group A and B, respectively. The DIP of the overlying water decreased significantly by 54.93% and 25.11% in group A and B, respectively. For the interstitial water in sediment, the DIP in layer Ⅰ (0-2 cm) decreased significantly by 74.81% and 65.66% in group A and B, and the DIP in layer Ⅱ (2-4 cm) decreased significantly by 46.23% in group B, but not obviously in group A. The DIP in layer Ⅲ (4-6 cm) remained unchanged in group A and B. For phosphorus fractions in sediment, the proportion of NH4Cl-P in TP was significantly increased (layer Ⅰ: 16.87% and 13.11%; layer Ⅱ: 12.99% and 11.02%, in group A and B, respectively), and the proportion of Al-P in TP was significantly decreased (layer Ⅰ: 7.58% and 13.91%; layerⅡ: 9.86% and 7.28%, in group A and B, respectively). The other phosphorus fractions did not change significantly. Both T-A and T-B dosing can improve the microbial activity of the surface sediment, though T-A can improve the microbial activity more significantly.

[ERK and P38MAPK expressions and human sperm motility].

OBJECTIVE: To compare the phosphorylation and protein expression of extracellular-signal regulated protein kinase (ERK) and P38 mitogen activated protein kinase (P38 MAPK) in the ejaculated spermatozoa of healthy volunteers and asthenospermia males, and to explore the correlation of ERK and P38 MAPK with human sperm motility. METHODS: Semen samples were collected from 20 healthy volunteers (sperm concentration > or = 20 x 10(6)/ml, grade a sperm > or = 25% or grade a + b sperm > or = 50%) and 20 infertile males with asthenospermia (sperm concentration > or = 20 x 10(6)/ml, grade a sperm < 25% and grade a + b sperm < or = 40%) and classified as a control and an asthenospermia group. Total protein in spermatozoa was extracted from all the subjects, and Western blotting was used to detect phosphorylation and protein expression levels of ERK and P38 MAPK. RESULTS: Compared with the control group, the protein expression levels of ERK and P38 MAPK and the phosphorylation level of P38 MAPK were significantly increased in the asthenospermia group (P < 0.05), but there were no statistically significant differences in the phosphorylation level of ERK between the two groups (P > 0.05). CONCLUSION: The up-regulated protein expressions of ERK and P38MAPK and increased phosphorylation level of P38 MAPK in human sperm may be involved in the pathogenesis of asthenospermia.