[Peptide composition of extracts of cryopreserved pigs' and piglets' heart fragments].

It is known that extracts of cryopreserved organ fragments of pigs and piglets stimulate the processes of reparative regeneration. Therefore, the study of the extracts is essential for understanding the mechanism of their biological effects. In this paper was studied the molecular mass distribution of peptides in the extracts of cryopreserved heart fragments of pigs' and piglets', i.e., the chromatograms of pigs' heart extracts show 3 picks, whilst piglets' heart extract show 6 picks. The peculiarity of pigs' heart extracts is the absence of peptides with molecular mass of 10,000 and more. The differences in the intensity of extracts fluorescence prove that the peptides in pigs' heart extracts contain greater amount of tryptophan residues accessible for solvent. The synchronous fluorescence spectra of extracts were obtained which allows the identification of extract without assessment of their components. Results shown in this research could be used for control and standardization of heart extracts peptide composition under investigation of their biological quality.

[Fluorescent probes for examination of spermatozoa in cryoprotective mediums].

By fluorescent spectroscopy and microscopy methods the possibility of fluorescent probes DSM, E-176, 3-DAB and FME application for study of cryoprotective agents' influence on the dog spermatozoa are investigated. It is established that FME and 3-DAD dyes are suitable for the posed problem solving, and the DSM and E-176 probes have restrictions owing to enough strong fluorescence from cryoprotectant solutions. It is shown that the fluorescent probes investigated influence the cells motility to different degree. The perspectives of investigated dyes application for study of cryoprotective agent' finfluence on spermatozoa are considered.

[Effect of temperature on the modification of spectral properties of tryptophan aqueous solutions induced by water previously treated with laser radiation].

It was shown that preliminary exposure of a solvent (water) to low-intensity laser radiation reduces the tryptophan fluorescence intensity, and this fluorescence quenching effect is retained throughout the temperature range explored (from 8 up to 50 degrees C). The effects found are interpreted as resulting from changes in solvent properties induced by the action of electromagnetic radiation on interaction of water molecules with solute.

[Effect of low-power laser irradiation on the structure and properties of protein molecules under non-specific energy absorbance]. / Vplyv nyz'koenerhetychnoho lazernoho vyprominiuvannia na strukturu ta vlastyvosti molekuly bilka za umov nespetsyfichnoho pohlynannia enerhiï.

The influence of the helium-neon laser emission on HSA was investigated at present work by means of the fluorescent probe MNBIS (4-morpholino-7-oxy-7-H-benzo-[de]-benzo-[4,5]-imidazo-[2,1-a]- izoquinolin-5-sulfonic acid) sensitive to protein conformational changes using. Fluorescence spectra parameters of the probe and kinetics of probe interaction with protein molecules were analyzed. Possible mechanisms of the helium-neon laser irradiation on water-protein solution structure are discussed.

[Effect of electron irradiation on the structure of microsomal membrane proteins]. / Vliianie élektronnogo oblucheniia na strukturu belkov mikrosomal'nykh membran.

Using tryptophan fluorescence quenching by iodide and acrylamide the effect of electrons with the energy 5 Mev on the structure of microsomal membrane proteins has been studied. Conformation of microsomal proteins were found to change upon irradiation.

[Interaction of 4-(n-dimethylaminostyryl)-1-methylpyridinium-n-toluolsulfonate with liposomes: analysis of fluorescence spectra]. / Vzaimodeistvie 4-(n-dimetilaminostiril)-1-metilpiridinii-n-toluolsul'fonata s liposomami: analiz spektrov fluorestsentsii.

Using fluorescent probe 4-(n-dimethylaminostyryl)-1-methylpyridinium n-toluenesulfonate the radiation effect on the structure of liposomes composed of phosphatidylcholine and diphosphatidylgycerol has been investigated. The from of fluorescence spectra was analyzed and inhomogeneous broadening parameters of spectral components were estimated. The polarity of DSM environment and probe distribution between different sites were found to change after irradiation.

[Study of radiation-induced change in the membrane structure using a fluorescent probe]. / Izuchenie indutsirovannogo radiatsiei izmeneniia struktury membran s pomoshch'iu fluorestsentnogo zonda.

The effect of radiation on erythrocyte membrane structure has been investigated using fluorescent probe 4-(n-dimethyl aminostyryl)-1-methylpyridinium n-toluolsulfonate. The form of fluorescence spectra was analyzed and inhomogeneous broadening parameters of spectral components were estimated. It was shown that in erythrocyte membrane there were three types of DSM binding sites. The physico-chemical properties of DSM surrounding and probes distribution were found to change after irradiation.

[Effect of ionizing radiation on the activity of microsomal oxidoreductases]. / Vliianie ioniziruiushchei radiatsii na aktivnost' mikrosomal'nykh oksidoreduktaz.

The effect of electrons with energy 5 MeV on the functional state of rabbit liver microsome oxidoreductases has been investigated. Decrease in enzyme activity upon irradiation with a dose of 10 kGy was found. The possible mechanisms of the effects observed are discussed.

[Study of the effect of low temperatures and organic solvents on the structure of model membranes using fluorescent probes]. / Issledovanie vliianiia nizkikh temperatur i organicheskikh rastvoritelei na strukturu model'nykh membran metodom fluorestsentnykh zondov.

By means of the fluorescence probes pyrene, DSP-12 and n-terphenyl the effect of low temperature, glycerol and 1,2-propylene glycol on the structure of lecithin liposomes and proteoliposomes formed by lecithin and cytochrome P-450 has been studied. Freezing-thawing and cryoprotectants were shown to modify surface area and internal volume of the lipid bilayer.