Active packaging for Salmon stored at refrigerator with Polypropylene nanocomposites containing 4A zeolite, ZnO nanoparticles, and green tea extract.

In this study, three types of Polypropylene-based (PP) films (two active nanocomposites and one control film) containing zinc oxide nanoparticles (ZnO NPs), 4A zeolite (4A Z), and green tea extract (GTE) were studied as modern active packaging's that can adjust the release of antimicrobial agents. The influence of PP nanocomposite with 3% (w/w) ZnO NPs/4A Z/GTE (treatment 1) and 6% (w/w) ZnO NPs/4A Z/GTE (treatment 2) on controlling microbial growth and preserving the sensory and chemical qualities of Salmon over nine days of storage at 4 ± 1°C was evaluated. The disk diffusion test revealed inhibition zones in the range of 10.98 ± 0.03 to 13.42 ± 0.01 m for treatments 1 and 2, respectively; the nanocomposite film with 6% ZnO NPs/4A Z/GTE had the highest antimicrobial effect against Gram-negative bacteria (p < .05). Chemical analysis revealed that the initial peroxide value of Salmon was 0.68 ± 0.0 mEq/kg, which increased by day 9 to 12.3 ± 0.03 mEq/kg in the control sample, but rising only to 9.9 ± 0.01 and 7.3 ± 0.02 mEq/kg in treatments 1 and 2, respectively (p < .05). The shelf life of Salmon given treatment 2 increased significantly to nine days relative to the control. Accordingly, these nanocomposite films are promising as new active packaging for preventing microbial growth and preserving the quality of salmon.

Polyvinyl alcohol/gelatin nanocomposite containing ZnO, TiO2 or ZnO/TiO2 nanoparticles doped on 4A zeolite: Microbial and sensory qualities of packaged white shrimp during refrigeration.

Recently, oxo-biodegradable polymers have attracted much attention due to taking less time to break down after disposal in comparison to ordinary polymers. Polyvinyl alcohol/gelatin (PVA/G) nanocomposite films, containing ZnO, TiO2 or ZnO/TiO2 nanoparticles supported on 4A zeolite (4A z), are novel active packaging that can control the release of antimicrobial compounds. The present study assessed the efficacy of PVA/G nanocomposite films with 1.5% (w/w) ZnO/4A z (treatment 1), 1.5% (w/w) TiO2/4A z (treatment 2), or 1% (w/w) ZnO, TiO2/4A z (treatment 3) in controlling the microbial load and maintaining the sensory qualities of white shrimp during storage at 4 ±â€¯1 °C. Firstly, the optimum concentration of each material for addition to the film was determined by micro-dilution and disc diffusion. Secondly, the specimens were checked for total viable count (TVC), as well as the counts of each of Pseudomonas spp., Enterobacteriaceae, Shewanella putrefaciens, inoculated Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli O157:H7. According to the results, the PVA/G nanocomposite films containing treatments 1-3 significantly decreased the number of bacteria in the treatment group in comparison to the control group (P < .05). The results of the antimicrobial activity of the three treatments by using the disc diffusion method revealed that the inhibition zone varied from 8.11 ±â€¯0.02 to 12.63 ±â€¯0.04 mm. Also it should be noted that, the finding of micro-dilution test varied from 1 ±â€¯0.01 to 3 ±â€¯0.01. The ZnO, TiO2/4A z nanocomposite had a significantly greater antimicrobial impact against Gram-negative bacteria compared to Gram-positive bacteria (P < .05). Finally, the microbiological and sensory investigation of the efficacy of the PVA/G nanocomposite films as active packaging materials revealed a considerable improvement in shrimp shelf life (12 days) in comparison to the control (6 days). Therefore, these nanocomposite films can be used as novel active packaging in the maintenance of the microbial load and sensory qualities of shrimp.