The Link between the Perception of Animal Welfare and the Emotional Response to Pictures of Farm Animals Kept in Intensive and Extensive Husbandry Systems: An Italian Survey.

As livestock production grows to satisfy the global demand for animal products, understanding public attitudes towards different husbandry systems becomes essential for both animal welfare and socio-economic reasons. This study aimed to investigate people's emotional responses toward pictures of farm animals kept in intensive and extensive husbandry systems, their perception of animal welfare, and their choices as animal product consumers. A questionnaire that included demographic questions and photos of cows, pigs, chickens, and rabbits in both intensive and extensive systems was distributed electronically and physically and completed by 835 respondents. Photos of animals in intensive systems elicited more negative emotions, especially for pigs and rabbits (p < 0.05), as opposed to extensive systems, which elicited more positive emotions, especially for chickens (p < 0.001). Higher welfare levels were perceived for extensively farmed animals (p < 0.001) and for cattle compared to all other species, regardless of the husbandry system (p < 0.001). The quality of the emotional response was positively associated with welfare perception (p < 0.001) and negatively associated with the importance given to welfare when purchasing animal products (p < 0.001). Finally, the emotional response was found to be affected by gender, education, household composition, living area, pet ownership, and eating habits. The implications and limitations of these findings are discussed.

Identification of Appropriate Endogenous Controls for Circulating miRNA Quantification in Working Dogs under Physiological Stress Conditions.

Cell-free miRNAs, called circulating miRNAs (cmiRNAs), can act in a paracrine manner by facilitating a diversity of signaling mechanisms between cells. Real-time qPCR is the most accepted method for quantifying miRNA expression levels. The use of stable miRNA endogenous control (EC) for qPCR data normalization allows an accurate cross-sample gene expression comparison. The appropriate selection of EC is a crucial step because qPCR data can change drastically when normalization is performed using an unstable versus a stable EC. To find EC cmiRNA with stable expression in search and rescue (SAR) working dogs, we explored the serum miRNome by Next-Generation Sequencing (NGS) at T0 (resting state) and T1 immediately after SAR performance (state of physiologically recovered stress). The cmiRNAs selected in the NGS circulating miRNome as probable ECs were validated by qPCR, and miRNA stability was evaluated using the Delta Ct, BestKeeper, NormFinder, and GeNorm algorithms. Finally, RefFinder was used to rank the stability orders at both T0 and T1 by establishing miR-320 and miR-191 as the best-circulating ECs. We are confident that this study not only provides a helpful result in itself but also an experimental design for selecting the best endogenous controls to normalize gene expression for genes beyond circulating miRNAs.

The Complex Interaction between P53 and miRNAs Joins New Awareness in Physiological Stress Responses.

This review emphasizes the important role of cross-talk between P53 and microRNAs in physiological stress signaling. P53 responds to stress in a variety of ways ranging from activating survival-promotion pathways to triggering programmed cell death to eliminate damaged cells. In physiological stress generated by any external or internal condition that challenges cell homeostasis, P53 exerts its function as a transcription factor for target genes or by regulating the expression and maturation of a class of small non-coding RNA molecules (miRNAs). The miRNAs control the level of P53 through direct control of P53 or through indirect control of P53 by targeting its regulators (such as MDMs). In turn, P53 controls the expression level of miRNAs targeted by P53 through the regulation of their transcription or biogenesis. This elaborate regulatory scheme emphasizes the relevance of miRNAs in the P53 network and vice versa.

Extracellular circulating miRNAs as stress-related signature to search and rescue dogs.

Our research explores serum extracellular circulating miRNAs (ecmiRNAs) involved in dog stress response immediately after the search and rescue (SAR) of missing people. The experimental plan considers four arduous SAR simulations. The SAR dogs are trained by the Alpine School of the Military Force of Guardia di Finanza (Passo Rolle, Italy). The First SAR Trial analyzed dog serum samples at rest time (T0), and immediately after SAR performance (T1) using the miRNome-wide screening next-generation sequencing (NGS). T1 versus T0 NGS results revealed a different expression level of let-7a and let-7f. Subsequently, in a large sample size including: 1st (n = 6), 2nd (n = 6), 3rd (n = 6), and 4th (n = 4) trials, let-7a and let-7f were validated by qPCR. Bioinformatics analysis with TarBase (v.8) and the Diana-mirPath (v.3) revealed a functional role of let-7a and let-7f in the p53 pathway to restore cellular homeostasis. Let-7a and let-7f, highly expressed at T1, could stop MDMs-p53 inhibition inducing the p53 increase in level. In addition, let-7a and let-7f, via p53 post-transcriptional regulation, buffers p53 transcription spikes. During SAR stress, the possibility of p53 preconditioning could explain the phenomenon of "stress hardening" where the tolerance of particular stress increases after preconditioning.

How Do Avalanche Dogs (and Their Handlers) Cope with Physical Exercise? Heart Rate Changes during Endurance in a Snowy Environment.

This study aimed to assess the heart rate (HR) responses of avalanche SAR dogs and handlers under working field conditions. Thirteen SAR units (dogs and handlers) performed an exercise (Endurance) consisting of approximately 5.5 km of rough tracks through deep snow, at an altitude of 1991-2250 m.a.s.l. The exercise was repeated twice for each of the two different tracks. Both handlers and dogs were equipped with a global positioning satellite/heart rate (GPS/HR) system (Polar®). Multivariable models were used to evaluate the effects of environmental (i.e., gradient, altitude, track, and time) and intrinsic (i.e., speed, repetition, and breed) factors on changes from baseline HR (Δ%HR). The dog's Δ%HR was greater in the flat and uphill compared with downhill, and increased progressively as the speed increased (p < 0.001). Moreover, it rose at altitudes above 2100 m.a.s.l. and peaked after 30 min of the Endurance activity (p < 0.01). These findings indicated that HR monitors could be a valuable tool to contribute to the evaluation of avalanche dogs' fitness in their real working environment. In contrast, the lack of correlation between the dogs' and handlers' HR changes suggests that handlers might not perceive the physical conditions of their dog in real-time. Thus, implementing protocols to monitor avalanche SAR dogs' fitness using a GPS/HR monitoring system could help handlers to tailor the training and workload and to detect the risk factors for physical distress of working dogs.

The Cat-Owner Relationship: Validation of the Italian C/DORS for Cat Owners and Correlation with the LAPS.

Globally, most people now own a pet. Scales have been developed to understand the impact of pet ownership on people's lives and to measure specific aspects of the owner-pet relationship. The Cat-/Dog-Owner Relationship Scale (C/DORS) is a tool developed to investigate this relationship in both dog and cat owners. The aim of the study was to refine and validate the C/DORS for cat owners in Italian. Exploratory Factor Analysis and Confirmatory Factor Analysis (CFA) were used to determine the most appropriate factor model. Construct validity was confirmed by correlating the C/DORS subscales with the Lexington Attachment to Pets (LAPS) subscales. Results confirmed the original structure of the English version (i.e., three factors: Pet Owner Interaction = POI, Perceived Emotional Closeness = PEC, Perceived Costs = PC) and CFA confirmed the structure of LAPS and C/DORS scales. Cronbach's alpha demonstrated the Italian version of the two scales to have good internal reliability for all domains. Owners of cats living exclusively indoors reported higher scores on POI and PEC compared to indoor/outdoor cats. Owning both cats and dogs was correlated with lower scores on POI, and fewer perceived costs (i.e., PC) of cat ownership. Finally, behaviour problems, not being neutered, and lack of previous experience with cat ownership were associated with lower scores on PC.

The Dog-Owner Relationship: Refinement and Validation of the Italian C/DORS for Dog Owners and Correlation with the LAPS.

The Cat/Dog-Owner Relationship Scale (C/DORS) can be administered to both dog and cat owners. However, the scale as a whole has never been validated on a sample of dog owners. Furthermore, it has never been translated into Italian. The aim of this study was to translate the C/DORS into Italian, modify its response scale in order to improve the degree of response variability, and test its validity and reliability on a sample of dog-owners. Exploratory factor analysis revealed the same three-factor structure (Perceived Emotional Closeness = PEC, Pet-Owner Interactions = POI, Perceived Costs = PC) as the original English version, although some items had to be removed because of low- or cross-loadings. The validity of the construct was confirmed by confirmatory factor analysis, by the correlations between each of the subscales and the C/DORS total score, and by the correlations with the Lexington Attachment to Pets Scale. Cronbach's α values for each subscale were above acceptable levels. Student owners scored higher on PEC and POI than owners with other occupations. Owners of dogs with behavioural problems scored lower on PEC and higher on PC. Keeping the dog outdoor was associated with lower POI. Finally, pet dog owners scored higher on PEC than AAI dog owners.

Overweight in Domestic Cats Living in Urban Areas of Italy: Risk Factors for an Emerging Welfare Issue.

Overweight is common in cats and has health and welfare implications. This study aimed to assess potential predictive/protective factors for feline overweight associated with owner management and their relationship with cat behavior and welfare. A questionnaire was administered to 197 owners to collect information about cat demographics, management, environment, dietary habits and behavior. The feline Body Mass Index was recorded for each cat. Univariate logistic regression was used to evaluate the association of parameters with overweight cats. Variables with a p-value < 0.10 at univariable analyses were selected for the multivariable model. Most cats were mixed- breed, 1-7 years old and neutered; 51.3% were overweight. Age higher than 7 years, neutering, being alone all day and being stressed were predictive for overweight (p < 0.05). Conversely, the presence of other animals had a protective effect (p < 0.05). A general disagreement between owners' perception and clinical evaluation of cats' body condition was a common and significant risk factor for overweight (OR = 8.532, 95% CI = 4.073-17.875; p < 0.001). According to the owners, many veterinarians did not inform them about their cat being overweight nor about the risks (p < 0.001). This study provides helpful information on the influence of management and environment on cats' body condition and its relationship with cat welfare.

Serotonin and Tryptophan Serum Concentrations in Shelter Dogs Showing Different Behavioural Responses to a Potentially Stressful Procedure.

In mammals, serotonin (5-HT) levels depend on the availability of tryptophan (TRP). Low 5-HT concentrations have been linked to behavioural disorders in dogs. This study aimed at investigating possible differences in dogs' serum TRP and 5-HT concentrations according to their behavioural response to a potentially stressful procedure. Thirty-nine physically healthy shelter dogs, 15 females and 24 males, mean age = 5.6 years, were categorized by a certified veterinary behaviourist according to their behavioural response to medical examination and blood collection, in: relaxation, stress signals, tension without growling, tension with growling, escape attempts, and aggression attempts. Extraction and quantification of 5-HT and TRP were performed using a HLPC method. Data were statistically analysed, applying Chi-square and Spearman tests. Results showed no significant difference in TRP (χ2 = 2.084, p = 0.555) nor 5-HT (χ2 = 0.972, p = 0.808) serum concentrations among different categories of dogs; however, some categories were underrepresented (relaxation = 20.5%, stress signals = 30.8%, tension without growling = 43.6%, tension with growling = 5.1%, escape attempts = 0%, aggression attempts = 0%). No correlation between serum TRP and 5-HT concentrations was found (ρ = 0.086, p = 0.602). Serum 5-HT levels do not seem to be associated with dogs' behavioural response to a stressful situation nor with serum TRP concentrations. The relationship between serum TRP and 5-HT concentrations and behaviour needs further research.

Cats and dogs: Best friends or deadly enemies? What the owners of cats and dogs living in the same household think about their relationship with people and other pets.

Although popular culture describes them as mortal enemies, more and more often, dogs and cats live under the same roof. Does this make them best friends? Can sharing the same social and physical environment make them similar? This study compares the approaches of dogs and cats living in the same household have towards humans and other pets as perceived by the owner. Questionnaires collected from 1270 people owning both dog(s) and cat(s) were analysed. Most dogs and cats living together are playful with familiar humans (76.2%) but dogs have a more sociable approach towards strangers and conspecifics than cats (P<0.001). Moreover, the percentage of dogs that have a playful relationship with the owner (84.0%) was higher than cats (49.2%; P<0.001). Dogs and cats living together eat in different places and show different mutual interactions: more dogs lick the cat (42.8%) and more cats ignore the dog (41.8%) than vice versa (P<0.001). However, most dogs and cats sleep at least occasionally (68.5%) and play together (62.4%; P<0.001). Although some body postures, such as the tail's position, are interpreted differently by the two species, the greater proportions of dogs and cats show a relaxed response to several kinds of approaches of their roommate. Our questionnaire confirms the common beliefs about the sociability of the dog and the privacy of the cat, but this does not result in continuous internal struggles. Most cohabitations are peaceful. Moreover, it is true that they speak different languages, but they seem to understand each other well and interpret each other's approaches in the right way. Thus, aspiring owners should not blindly believe popular assumptions, but both knowledge and respect for species-specific pet behaviours are essential to establish a balance in the household.

Omics Insights into Animal Resilience and Stress Factors.

Resilience is conceived as a dynamic developmental process involving the achievement of positive adaptation within the context of significant adversity. Resilience is not a unique ability but rather a set of capacities of a system put in place to absorb a disturbance and to reorganize while trying to retain the same function, structure, and identity. This review describes the characteristics and the molecular mechanisms of resilience to understand the core elements of resilience and its indicators. The objectives of this review are: (1) to define some of the leading environmental stressors and clarify the mechanism of vulnerability or resilience outcomes; (2) to clarify some of the prominent epigenetic modulations mediating resilience or vulnerability as a stress response; (3) to highlight the neural mechanisms related to stress resilience since the central nervous system is a highly dynamic structure characterized by an everlasting plasticity feature, which therefore has the opportunity to modify resilience. The review aims to introduce the reader to the concept of resilience seen as an ability acquired in life and not only inherited from birth.

Benefits of dietary supplements on the physical fitness of German Shepherd dogs during a drug detection training course.

A high standard of physical fitness is an essential characteristic of drug detection dogs because it affects not only their ability to sustain high activity levels but also their attention and olfaction efficiency. Nutritional supplements could improve physical fitness by modulating energy metabolism, oxidative processes, and perceived fatigue. The aim of this study was to investigate the physiological and biochemical changes induced by submaximal exercise on drug detection dogs (German Shepherd breed) and to assess whether a dietary supplement improves their physical fitness. During a drug detection dog training course, seven dogs were fed with a basal diet (Control Group) for three-month period, while a further seven dogs were fed with a basal diet as well as a daily nutritional supplement containing branched-chain and limiting amino acids, carnitine, vitamins, and octacosanol (Treatment Group). At the end of this period, individual physical fitness was assessed by making each subject take a graded treadmill exercise test. A human heart rate monitor system was used to record the dog's heart rate (HR) during the treadmill exercise and the subsequent recovery period. The parameters related to HR were analysed using nonparametric statistics. Blood samples were collected before starting the nutritional supplement treatment, before and after the treadmill exercise and following recovery. Linear mixed models were used. The dietary supplements accelerated HR recovery, as demonstrated by the lower HR after recovery (P<0.05) and Time constants of HR decay (P<0.05), and by the higher Absolute HR Recovered (P<0.05) recorded in the Treatment group compared with the Control dogs. The supplemented dogs showed the lowest concentrations of creatine kinase (CK; P<0.001), aspartate aminotransferase (AST, P<0.05) and non-esterified fatty acids (NEFA; P<0.01) suggesting a reduction in muscle damage and improvement of energy metabolism. These data suggest that this combined supplement can significantly enhance the physical fitness of drug detection dogs.

Welfare Assessment in Shelter Dogs by Using Physiological and Immunological Parameters.

This study aimed to evaluate the state of welfare of a group of dogs during the first month after entering the shelter by using different stress parameters. Blood and fecal samples were collected from a group of 71 dogs at the time of admission to the shelter. In 46 of these dogs, sampling was repeated after four weeks. Well-recognized welfare biomarkers, such as fecal cortisol and leukocytes, as well as some innovative parameters (ß-endorphin and lysozyme) were determined. Uni- and multivariate statistical analyses were used to evaluate their interactions and changes over time. Neutrophils (p < 0.01), lysozyme (p < 0.05), and fecal cortisol (p < 0.05) decreased, while lymphocytes (p < 0.05) increased after four weeks compared to the first days of being in the shelter, suggesting an improvement in the dogs' welfare over time. A principal component analysis extracted three bipolar components (PCs), explaining 75% of the variance and indicating negative associations between neutrophil and lymphocyte (PC1), lysozyme and ß-endorphin (PC2), cortisol and lysozyme (PC3). The associations between these variables within each PC also confirmed the intricate relationships between the hypothalamic-pituitary-adrenal (HPA) axis and the immune system as well as the importance of a multiparametric approach in evaluating welfare.

Attitudes toward Animals and Their Welfare among Italian Veterinary Students.

As members of the public and the veterinary profession are increasingly concerned about animal welfare, there has been an increased scholarly interest in the attitudes of veterinarians and students toward animals, as these may impact human behavior, which ultimately impacts animal welfare. Here we investigated Italian veterinary students' demographic data and perceptions about nonhuman animal welfare issues that might be predictive of their attitudes. A survey eliciting information about demographics, knowledge, experience, and perceptions regarding different categories of animals, and including the Animal Attitude Scale (AAS), was administered to undergraduate veterinary medicine students in three Italian universities. Data were analyzed using nonparametric tests, and a value of p < 0.05 was considered statistically significant. In total, 876 students completed the questionnaire, with females (75.1%) making up a majority of students in all years of the course. Although veterinary students showed pro-animal welfare attitudes (mean score = 64.20 ± 0.24 out of 100), the findings suggested that year of study, gender, and geographical location had a significant impact (p < 0.05). In this study, we found a set of factors that, either individually or combined, help predict a student's attitude toward animal welfare issues, which will be useful in improving the curriculum strategy in veterinary education in Italy.

Familiarity and Interest in Working with Livestock Decreases the Odds of Having Positive Attitudes towards Non-Human Animals and Their Welfare among Veterinary Students in Italy.

We investigated the attitudes of veterinary students towards animals and their welfare in Italy. Regression analyses revealed predictors that are significant in differentiating students' scoring tendency based on their gender, familiarity, and intention to work with a specific animal species, type of diet, and membership in an animal rights association. Female students, who were mostly familiar with pets and aspired to work with species other than livestock, following an animal-free diet and being a member of an animal rights association, had a significantly greater odds of having a high Animal Attitude Scale score (AAS), i.e., very positive attitude towards animals, versus a less positive attitude. Conversely, the familiarity with livestock and preference for working with livestock significantly increased the odds of a low AAS. Overall, students considered all of the Brambell Report's Five Freedoms important for animal welfare protection. However, students scored higher for companion animals than for livestock, particularly regarding the freedom to express normal behaviour and the absence of fear and distress. This study suggests that veterinary students place less importance on the psychological aspects of welfare for livestock, and there is a tendency for students who are mostly familiar, or aspire to work, with livestock to have a less positive attitude towards non-human animals and their welfare. These findings should be considered within the veterinary educational curriculum due to their potential impact on animal welfare.

My Dog Is Not My Cat: Owner Perception of the Personalities of Dogs and Cats Living in the Same Household.

This study aims to define the personality traits perceived by the owners of multiple pets and to evaluate how they are modulated by experiential-environmental factors. A questionnaire was administered to 1270 owners of multiple pets (dogs and cats) to collect data on the demographics, management, and personality of their pets. Data were analysed by principal component analysis, bivariate, and multivariable models. Five personality traits emerged in dogs and cats: sociability, reactivity, protectiveness, neuroticism, and fearfulness. The owners perceived differences in the personality of their pet: dogs scored higher in sociability, protectiveness, and reactivity, while lower in the neuroticism dimension compared with cats (p < 0.001). Age similarly affected sociability (p < 0.01), and reactivity (p < 0.001) in both dogs and cats, while species-specific gender differences were found as to fearfulness (p < 0.05) and neuroticism (p < 0.001). The age of acquisition modulated several traits in dog personality, while living with conspecifics especially influenced cats. Physiological, behavioural, and evolutionary characteristics could explain species differences. Moreover, intrinsic and extrinsic factors modulated the five dimensions of dogs and cats in a diversified fashion, suggesting complex interactions between species and the environment. However, owners could have had different attitudes with their animals which could have influenced personality perception.

Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research.

There is emerging evidence that microRNAs (miRNAs) dysregulation is involved in the genesis and the progression of Prostate Cancer (PCa), thus potentially increasing their use in urological clinical practice. This is the first pilot study which utilizes Illumina Deep Sequencing to examine the entire miRNAs spectrum existent in urine exfoliated prostate cells (UEPCs) of PCa patients. A total of 11 male patients with histological diagnosis of PCa were enrolled in the present study. First-catch urine (30 mL) was collected following a prostate massage. Total RNA was extracted from urine and sequenced using an HiSeq2500 System (Illumina). QPCR assay was used to validate the highest NGS results in PCA patients and in age-matched, caucasian men. Remarkably, PCA let-7 family was down-regulated (P < 0.01), compared to the controls. The results of our study support the notion of a relatively high diagnostic value of miRNA family for PCa detection, especially in the let-7 family. The present research confirmed the potential use of miRNAs as non-invasive biomarkers in the diagnosis of PCa, potentially reducing the invasiveness of actual clinical strategy.

A simulated avalanche search and rescue mission induces temporary physiological and behavioural changes in military dogs.

Saving human lives is of paramount importance in avalanche rescue missions. Avalanche military dogs represent an invaluable resource in these operations. However, their performance can be influenced by several environmental, social and transport challenges. If too severe, these are likely to activate a range of responses to stress, which might put at risk the dogs' welfare. The aim of this study was to assess the physiological and behavioural responses of a group of military dogs to a Simulated Avalanche Search and Rescue mission (SASR). Seventeen avalanche dogs from the Italian Military Force Guardia di Finanza (SAGF dogs) were monitored during a simulated search for a buried operator in an artificial avalanche area (SASR). Heart rate (HR), body temperature (RBT) and blood samples were collected at rest the day before the trial (T0), immediately after helicopter transport at the onset of the SASR (T1), after the discovery of the buried operator (T2) and 2h later (T3). Heart rate (HR), rectal body temperature (RBT), cortisol, aspartate aminotransferase (AST), creatine kinase (CK), non-esterified fatty acids (NEFA) and lactate dehydrogenase (LDH) were measured. During the search mission the behaviour of each SAGF dog was measured by focal animal sampling and qualitatively assessed by its handler and two observers. Inter-rater agreement was evaluated. Snow and environmental variables were also measured. All dogs successfully completed their search for the buried, simulated victim within 10min. The SASR was shown to exert significant increases on RBT, NEFA and cortisol (P<0.001), CK and HR (P<0.01), AST and LDH (P<0.05). These indicate the activation of a response to stress probably induced by the addition of factors such as helicopter transport, disembarking, and the search and rescue exercise. However, changes were moderate and limited over time, progressively decreasing with complete recovery at T3 except for sera cortisol that showed a slightly slower decline. More time walking within the search was related to lower RBT, conversely to walking. Standing still with head up and exploring with head-up were inversely related with HR. Agreement between handler and observers' opinions on a dog's search mission ability was found only for motivation, signalling behaviour, signs of stress and possessive reward playing. More time signalling was related to shorter search time. In conclusion, despite extreme environmental and training conditions only temporary physiological and behavioural changes were recorded in the avalanche dogs. Their excellent performance in successful simulated SASR may be attributable to extensive training and good dog-handler relationships. Simulated SASR did not seem to impair SAGF dogs' performance or welfare.

Infections and risk factors for livestock with species of Anaplasma, Babesia and Brucella under semi-nomadic rearing in Karamoja Region, Uganda.

A survey was conducted to estimate the prevalence of Anaplasma, Babesia and Brucella spp. infections in cattle, goats and sheep in the Karamoja Region of Uganda and to identify possible risk factors existing in this semi-nomadic and pastoral area. Low cost laboratory tests were used to diagnose infections (Rose Bengal test for Brucella spp. antibodies and direct microscopic examination for Anaplasma and Babesia spp.). Multivariable logistic regression models were applied to identify possible risk factors linked to gender, animal species, age (only for cattle) and districts. A total of 3935 cattle, 729 goats and 306 sheep of five districts of the Karamoja Region were tested. Seroprevalence for Brucella was 9.2 % (CI, 95 %: 8.4-10), for Anaplasma 19.5 % (CI 95 %: 18.4-20.6) and for Babesia 16 % (CI 95 %: 15-17.1). Significant differences in infections prevalence were observed against risk factors associated with districts and species. Cattle were the species with higher risk of the infections. Female gender was identified as at risk only for Brucella spp. infection. Cattle more than one year old had greater likelihood to be Brucella seropositive. Co-infections of Anaplasma and Babesia spp. were statistically associated, especially in goats and sheep. Further studies to identify risk factors related to host species and geographical districts are needed. The influence on the semi-nomadic agro-pastoral system in Karamoja of animal raids and animal mixing should be further investigated. Findings were important to sensitize Karamojong undertaking measures on infection control, especially on cattle, which are their main source of food.

RandAgiamo™, a Pilot Project Increasing Adoptability of Shelter Dogs in the Umbria Region (Italy).

Current Italian legislation does not permit euthanasia of dogs, unless they are ill or dangerous. Despite good intentions and ethical benefits, this 'no-kill policy' has caused a progressive overpopulation of dogs in shelters, due to abandonment rates being higher than adoption rates. Shelter overcrowding has negative implications for dog welfare and increases public costs. The aim of this paper is to describe the pilot project "RandAgiamo" implemented in a rescue shelter in the Umbria Region and to evaluate its effectiveness on the rate of dog adoption using official data. RandAgiamo aimed to increase adult shelter dogs' adoptability by a standard training and socialization programme. It also promoted dogs' visibility by publicizing them through social media and participation in events. We analysed the official data of the Umbria regional health authorities regarding dog shelters of the Perugia province of the year 2014. In the RandAgiamo shelter, the dog adoption rate was 27.5% higher than that of dogs housed in other shelters located in the same geographical area (P < 0.001). The RandAgiamo project could be beneficial for the dogs' welfare, owner satisfaction, shelter management, and public perception of shelter dogs. However, staff were required to provide dog training and related activities.