Assessment of Mechanical Damage and Germinability in Flaxseeds Using Hyperspectral Imaging.

The high demand for flax as a nutritious edible oil source combined with increasingly restrictive import regulations for oilseeds mandates the exploration of novel quantity and quality assessment methods. One pervasive issue that compromises the viability of flaxseeds is the mechanical damage to the seeds during harvest and post-harvest handling. Currently, mechanical damage in flax is assessed via visual inspection, a time-consuming, subjective, and insufficiently precise process. This study explores the potential of hyperspectral imaging (HSI) combined with chemometrics as a novel, rapid, and non-destructive method to characterize mechanical damage in flaxseeds and assess how mechanical stresses impact the germination of seeds. Flaxseed samples at three different moisture contents (MCs) (6%, 8%, and 11.5%) were subjected to four levels of mechanical stresses (0 mJ (i.e., control), 2 mJ, 4 mJ, and 6 mJ), followed by germination tests. Herein, we acquired hyperspectral images across visible to near-infrared (Vis-NIR) (450-1100 nm) and short-wave infrared (SWIR) (1000-2500 nm) ranges and used principal component analysis (PCA) for data exploration. Subsequently, mean spectra from the samples were used to develop partial least squares-discriminant analysis (PLS-DA) models utilizing key wavelengths to classify flaxseeds based on the extent of mechanical damage. The models developed using Vis-NIR and SWIR wavelengths demonstrated promising performance, achieving precision and recall rates >85% and overall accuracies of 90.70% and 93.18%, respectively. Partial least squares regression (PLSR) models were developed to predict germinability, resulting in R2-values of 0.78 and 0.82 for Vis-NIR and SWIR ranges, respectively. The study showed that HSI could be a potential alternative to conventional methods for fast, non-destructive, and reliable assessment of mechanical damage in flaxseeds.

Detection of Coconut Clusters Based on Occlusion Condition Using Attention-Guided Faster R-CNN for Robotic Harvesting.

Manual harvesting of coconuts is a highly risky and skill-demanding operation, and the population of people involved in coconut tree climbing has been steadily decreasing. Hence, with the evolution of tree-climbing robots and robotic end-effectors, the development of autonomous coconut harvesters with the help of machine vision technologies is of great interest to farmers. However, coconuts are very hard and experience high occlusions on the tree. Hence, accurate detection of coconut clusters based on their occlusion condition is necessary to plan the motion of the robotic end-effector. This study proposes a deep learning-based object detection Faster Regional-Convolutional Neural Network (Faster R-CNN) model to detect coconut clusters as non-occluded and leaf-occluded bunches. To improve identification accuracy, an attention mechanism was introduced into the Faster R-CNN model. The image dataset was acquired from a commercial coconut plantation during daylight under natural lighting conditions using a handheld digital single-lens reflex camera. The proposed model was trained, validated, and tested on 900 manually acquired and augmented images of tree crowns under different illumination conditions, backgrounds, and coconut varieties. On the test dataset, the overall mean average precision (mAP) and weighted mean intersection over union (wmIoU) attained by the model were 0.886 and 0.827, respectively, with average precision for detecting non-occluded and leaf-occluded coconut clusters as 0.912 and 0.883, respectively. The encouraging results provide the base to develop a complete vision system to determine the harvesting strategy and locate the cutting position on the coconut cluster.

Evaluation of Effective Class-Balancing Techniques for CNN-Based Assessment of Aphanomyces Root Rot Resistance in Pea (Pisum sativum L.).

Aphanomyces root rot (ARR) is a devastating disease that affects the production of pea. The plants are prone to infection at any growth stage, and there are no chemical or cultural controls. Thus, the development of resistant pea cultivars is important. Phenomics technologies to support the selection of resistant cultivars through phenotyping can be valuable. One such approach is to couple imaging technologies with deep learning algorithms that are considered efficient for the assessment of disease resistance across a large number of plant genotypes. In this study, the resistance to ARR was evaluated through a CNN-based assessment of pea root images. The proposed model, DeepARRNet, was designed to classify the pea root images into three classes based on ARR severity scores, namely, resistant, intermediate, and susceptible classes. The dataset consisted of 1581 pea root images with a skewed distribution. Hence, three effective data-balancing techniques were identified to solve the prevalent problem of unbalanced datasets. Random oversampling with image transformations, generative adversarial network (GAN)-based image synthesis, and loss function with class-weighted ratio were implemented during the training process. The result indicated that the classification F1-score was 0.92 ± 0.03 when GAN-synthesized images were added, 0.91 ± 0.04 for random resampling, and 0.88 ± 0.05 when class-weighted loss function was implemented, which was higher than when an unbalanced dataset without these techniques were used (0.83 ± 0.03). The systematic approaches evaluated in this study can be applied to other image-based phenotyping datasets, which can aid the development of deep-learning models with improved performance.