RESUMO
The chemical composition of the essential oil obtained from the aerial parts of Santolina chamaecyparissus L., growing in Algeria, was investigated by GC-MS analyses. A total of 36 compounds were identified, accounting for 91.7% of the essential oil obtained. Camphor (31.1%) and cubenol (17.0%) were the predominant compounds. The potential of the antimicrobial activity was also investigated and the tested sample proved to be very active against Klebsiella pneumonia and Candida albicans (34.1 +/- 0.02 mm and 35.0 +/- 0.01 mm, respectively). Transverse sections of the leaf and stem of the plant suggest that the essential oil is localized in endogenous and exogenous sites.
Assuntos
Anti-Infecciosos/farmacologia , Asteraceae/química , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Argélia , Anti-Infecciosos/química , Candida albicans/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Klebsiella pneumoniae/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Folhas de Planta/química , Caules de Planta/química , Terpenos/química , Terpenos/farmacologiaRESUMO
A novel elemanolide with an alpha-methyl-gamma-lactone moiety, 8alpha-O-(4-hydroxy-2-methylenebutanoyloxy)melitensine, in addition to four known sesquiterpene lactones also bearing the same lactone ring, melitensin, 11beta,13 dihydrosalonitenolide, 8alpha-hydroxy-11beta,13-dihydro-4-epi-sonchucarpolide, and 8alpha-hydroxy-11beta,13-dihydro-onopordaldehyde have been isolated from the aerial parts of Centaurea pullata. The in vitro antibacterial and antifungal activities of the isolated sesquiterpene lactones were tested against six bacteria and eight fungal species, using a microdilution method. All compounds tested showed greater antibacterial and antifungal activities than the positive controls used. Moreover, the pharmacokinetic profile of these compounds was investigated using computational methods.
Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Centaurea/química , Lactonas/química , Lactonas/farmacologia , Sesquiterpenos/química , Antibacterianos/isolamento & purificação , Lactonas/isolamento & purificação , Espectroscopia de Ressonância Magnética , Viabilidade Microbiana/efeitos dos fármacos , Estrutura Molecular , Relação Estrutura-AtividadeRESUMO
The aerial parts of Centaurea pullata afforded, in addition to the previously isolated sesquiterpene lactones, 11beta,13-dihydrocnicin and 11beta,13-dihydro-19-desoxycnicin, three minor sesquiterpene lactones, namely, a new germacranolide, 8alpha-O-(4-acetoxy-5-hydroxyangeloyl)-11beta,13-dihydrocnicin, and two new eudesmanolides, 8alpha-O-(4-hydroxy-2-methylenebutanoyloxy)-11beta,13-dihydrosonchucarpolide and 8alpha-O-(4-hydroxy-2-methylenebutanoyloxy)-11beta,13-dihydro-4-epi-sonchucarpolide. The in vitro antimicrobial activity of all isolated sesquiterpene lactones was tested against six bacteria and eight fungal species, using a microdilution method. All compounds tested showed greater antibacterial and antifungal activities than the positive controls used.
