[Detection of human papillomavirus (H.P.V.) DNA in genital lesions using molecular hybridization]. / Détection du DNA des virus du papillomme humain (H.P.V.) dans les lésions génitales par hybridation moléculaire.

Detection of human papilloma virus in genitals lesions by molecular hybridization. Some H.P.V. types are sexually transmitted and infect genital organs. We have used molecular hybridization to examine the distribution of H.P.V. 6 or II and H.P.V. 16 in benign, premalignant and malignant genital lesions from 344 patients. The frequency of H.P.V. 16 positive cases increases as the cervical lesions progress to malignancy: 57/78 are positive (73%) in the carcinomas, 29/83 are positive (35%) in mild or moderate dysplasia. The majority of benign condylomata acuminata harbors DNA of other types, namely H.P.V. 6 and II.

Chlamydial genital infection in Algiers: a sero-epidemiological survey.

Using a microimmunofluorescence test, the prevalence of antichlamydial immunoglobulin (Ig) G in 720 people in Algiers was studied. 34 (36%) of women with low genital infection, 28 (30%) of 91 patients attending a cancer screening clinic, and 44 (100%) of prostitutes had antichlamydial IgG at a titre greater than or equal to 1:16. Among 180 women seeking a rubeola test, 48 (26.6%) had IgG titres greater than or equal to 1:16. 144 infants less than 3 months old were also tested and 16.6% of them had IgG titres greater than or equal to 1:160; 20 (20.7%) of 97 men with chronic urethritis had IgG titres greater than or equal to 1:16. Antibody titres suggesting active disease in prostitutes, patients attending the cancer screening clinic and women with low genital infection were found in 95%, 11% and 17% respectively.

[Herpes simplex virus infections in Algiers]. / Infections a virus herpes simplex a Alger.

The purpose of this study was to determine the prevalence of herpes simplex virus antibodies among the population in Algiers. Anti-bodies to HSV1 are acquired rapidly between the ages of 1 and 6 years and 81.25% of the population is HSV1 seropositive by 15 years of age. Patients suffering from genital disorders possess HSV type 2 antibodies at a rate significantly higher (p less than 0.001) than in the control group.

Herpetic and chlamydial genital infections in Algiers, Algeria: value of the Papanicolaou staining.

The prevalence of herpetic and chlamydial genital infections was studied in 309 women attending an outpatient gynecology clinic (Algiers, Algeria). Cell culture, IF on smears and Papanicolaou staining were the methods used for HSV diagnosis, serology by the MIF method and Papanicolaou staining were used for Chlamydia trachomatis diagnosis. The prevalence of herpetic genital infection was estimated at 7.2% by cell culture, 89% by IF method, and 3.9% using the Papanicolaou staining. Specific anti-Chlamydia trachomatis IgG with a titer greater than or equal to 16 were found in 35.7% out of 95 sera, and 18% out of the 95 women had an IgG titer greater than or equal to 64. If we consider IgG titer greater than or equal to 64 as significant for an active chlamydial genital infection, the concordance rate between the Papanicolaou staining and the MIF method is of 48%. However, the Papanicolaou method appears as insensitive for both the HSV and Chlamydia trachomatis diagnosis. The relative specificity of this method for HSV diagnosis accounts for its use as a screening test.