Genetic diversity of Banat Naked Neck, indigenous chicken breed from Serbia, inferred from mitochondrial DNA D-loop sequence and microsatellite markers.

Banat Naked Neck is the most important indigenous breed of chickens in Serbia. Marginalized until recently, it is becoming increasingly popular due to its adaptability and good productivity in alternative production systems. However, its history and the current breeding model pose challenges for breed preservation and future improvement. This study aimed to assess the genetic diversity and structure of four subpopulations of Banat Naked Neck from different districts in Serbia (West Backa, North Banat, South Banat and Kolubara) using D-loop mitochondrial DNA sequences and a set of 30 microsatellite markers. Seven haplotypes in the phylogenetic analysis of D-loop mitochondrial DNA suggested maternal origin related to the Indian subcontinent, while haplotype and nucleotide diversity averaged 0.731 ± 0.053 and 0.0067 ± 0.0018, respectively. Microsatellite genotyping showed an average detected number of alleles per locus of 5.129 ± 0.237, while the observed and expected heterozygosity averaged 0.560 ± 0.018 and 0.631 ± 0.014, respectively. Genetic differentiation estimated through FST was 0.051 (p < .001). Two clusters in STRUCTURE analysis showed possible separation of two older subpopulations (South Banat and Kolubara) from the two more recent ones (West Backa and North Banat). This first comprehensive study of genetic diversity serves as the basis for future preservation, use and improvement of the Banat Naked Neck breed.

Welfare Assessment on Different-Sized Dairy Goat Farms in the Northern Serbian Province of Vojvodina.

This research aimed for the first time to identify the essential welfare problems on 46 goat dairy farms in Vojvodina,based on the goat AWIN welfare protocol. The study showed that space allowance per goat was 1.55, 1.86 and 2.50 m2/goat (large, medium, small-sized farms, respectively). Most farms possessed the soil floor and straw as a flooring material. The small and medium farms more allowed access to the outdoor area. Contrary, large-sized farms mostly kept goats in fully housed systems with reduced opportunities for the goats to express their natural behavior. Management procedures were significantly different (p < 0.05) between the investigated farms. Differences were mainly in bedding, feeding and culling strategies. Low productivity was the main cause of culling in all farm types. Small-sized farms carried out more often disbudding (42.9%) in comparison with medium (21.4%) and large-sized ones (14.3%). Group level observation showed that the main areas of concern were associated with hair coat condition and kneeling at the feeding rack (p < 0.05). Inadequate BCS, udder asymmetry and claw overgrowth were frequently recorded. The prevalence of thin and fat goats was highest in large-sized farms All farm size categories and management systems showed certain welfare weaknesses. Thus, the findings highlighted the need for higher implementation of animal welfare principles and improvement of management techniques to meet the welfare requirements of dairy goats.