RESUMO
An available enzyme-linked immunosorbent assay (ELISA) was studied for the detection of anti-Dioctophyma renale antibodies in the sera of dogs using, detection of parasite eggs in urine sediment as a reference test. ELISA uses a soluble antigenic preparation of esophagus of D. renale and the optimal dilutions of the antigen, serum and conjugate were determined by means of checker board titration, using positive (n=13) and negative (n=27) reference serum. The specificity and sensitivity of the ELISA were 93.8% and 92.3% respectively and the kappa index was good (0.76). These results suggest that ELISA described may prove to be an effective serological test for detecting dogs infected and exposed to this parasite mainly dogs that are not eliminating parasite eggs through their urine.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Dioctophymatoidea/imunologia , Doenças do Cão/parasitologia , Infecções por Enoplida/veterinária , Ensaio de Imunoadsorção Enzimática/veterinária , Animais , Antígenos de Helmintos , Doenças do Cão/diagnóstico , Cães , Infecções por Enoplida/sangue , Infecções por Enoplida/diagnóstico , Infecções por Enoplida/parasitologia , Feminino , Masculino , Sensibilidade e Especificidade , Testes Sorológicos/métodos , Testes Sorológicos/veterináriaRESUMO
Dioctophyme renale larvae have been found in cysts in the gastric wall of 5.17% (3/58) Chaunus ictericus specimens from São Cristóvão district, Três Barras municipality, Santa Catarina state, Brazil. However, larvae of this nematode were not found in sympatric Chaunus schneideri. The larvae caused a mild granulomatous reaction. This is the first report of paratenic hosts for D. renale in Brazil, and probably is also the first in the Neotropical region.
Assuntos
Bufonidae/parasitologia , Infecções por Enoplida/veterinária , Enoplídios/fisiologia , Animais , Brasil/epidemiologia , Enoplídios/citologia , Infecções por Enoplida/epidemiologia , Infecções por Enoplida/parasitologia , Infecções por Enoplida/patologia , Feminino , Larva/citologia , Masculino , PrevalênciaRESUMO
This study aims to provide information on morphological data of D. renale eggs, as well as on first-stage larvae development into eggs kept at different temperatures. Eggs were obtained by centrifugation of infected dog urine, placed in Petri dishes, and stored in BOD chamber for a 90-day period. Each treatment (GI--15 degrees C, GII--20 degrees C, and GIII--26 degrees C) was repeated five times. Eggs showed average measures of 67.23 x 42.78 microm, and the mean incubation time was inversely proportional to the incubation temperature. Larvae motility was observed one week after being observed in eggs.
Assuntos
Dioctophymatoidea/embriologia , Animais , Larva/crescimento & desenvolvimento , Óvulo/citologia , TemperaturaRESUMO
Fifty eight Chaunus ictericus and 42 C. schneideri specimens were collected on São Cristóvão district, Três Barras, SC for helminthological studies. Fourteen helminth species were diagnosed, from which only five species were found on both hosts. Chaunus ictericus showed higher values of species richness (2,8448+/-1,1516) and diversity (H = 1,374), with mild dominance (1-D = 0,642, J = 0,5528), in comparison with C. schneideri (0,6428+/-1,007; H = 1,165; 1-D = 0,5822 e J = 0,5985). Also, descriptors of helminthic infection were superior in the former host. Little number of shared species between the analyzed toad species suggests parasitic host-specificity.
Assuntos
Bufonidae/parasitologia , Helmintos/isolamento & purificação , Animais , BrasilRESUMO
This study evaluated the gastrointestinal helminth fauna of long-nosed armadillos, Dasypus novemcinctus, from the Pantanal wetlands, Aquidauana sub-region, Aquidauana County, Mato Grosso do Sul State, Brazil. Thirteen species of nematodes, comprising seven genera and four families, were recovered from their gastrointestinal tracts. The following descriptors of infection were determined: prevalence, variation of intensity, average intensity and abundance. Hadrostrongylus speciosum n. gen. et n. sp. is first described here.