RESUMO
The theoretical basis of this work is the development of autosensitization in exposure to ionizing radiation and well-known desensitizing action of sulphuric agents. To reduce clinical manifestations of a reaction to combined radiotherapy 34 women with diagnosis of cervical cancer (stages I and II) were given 0.5-1 g of purified sulphur mixed with 0.25 g of glucose in the morning every 2-3 hours before irradiation, per os; 24 patients received placebo (a mixture of egg powder with glucose), in 21 patients no protective means were used. All 79 patients were given unified adjuvant therapy (polyvitamins, leukogens, folic acid) and diet No 15. A significant decrease in the reaction to therapeutic irradiation was noted in the study group. No side-effects were observed.
Assuntos
Lesões Experimentais por Radiação/prevenção & controle , Lesões por Radiação/prevenção & controle , Protetores contra Radiação , Enxofre/uso terapêutico , Neoplasias do Colo do Útero/radioterapia , Animais , Cães , Avaliação de Medicamentos , Avaliação Pré-Clínica de Medicamentos , Quimioterapia Combinada , Feminino , Humanos , Masculino , Lesões por Radiação/etiologia , Lesões Experimentais por Radiação/etiologia , Dosagem Radioterapêutica , Enxofre/efeitos adversos , Enxofre/isolamento & purificação , Neoplasias do Colo do Útero/complicaçõesRESUMO
It has been demonstrated in experiments on 2160 CBA mice and mice of mixed breed, irradiated by LD90/30, that therapeutical administration (subcutaneous or intraperitoneal) of immunoglobulins - homologous (polyglobulin, IgG, IgM) or heterologous - polyglobulin, IgG (from human, equine, canine blood) repeated three times, i.e., 2, 24 and 48 hours after irradiation, not only induces longer survival, but also shows a normalizing effect on the commonly developing dysbacteriosis and increased amount of intestinal microflora and, in addition, leads to suppression of postirradiation endogenous infection. Enterobacteria, enterococci, staphylococci, yeasts, disappear from the small intestine or their quantity decreases substantially in treated irradiated mice in contrast to untreated irradiated mice. In the large intestine, the amount of these organisms decreases considerably while the content of lactobacteria increases; no microbes can be found in the internal organs and in blood (or their content is small). Other conditions being equal, homologous immunoglobulins are more efficient in comparison with heterologous, this applying also to preparations containing normal antibodies to tissues.
Assuntos
Infecções Bacterianas/terapia , Imunização Passiva , Intestinos/microbiologia , Lesões Experimentais por Radiação/complicações , Animais , Bactérias/crescimento & desenvolvimento , Infecções Bacterianas/etiologia , Feminino , Imunoglobulina G/uso terapêutico , Imunoglobulina M/uso terapêutico , Intestino Grosso/microbiologia , Intestino Delgado/microbiologia , Masculino , Camundongos , Camundongos Endogâmicos CBA , Irradiação Corporal TotalRESUMO
The therapeutic application of native and in vitro exposed (20000 Gy immunoglobulins of horse blood serum increased the survival of irradiated (LD75-95) animals, normalized the quantitative and qualitative status of the small intestine microflora and prevented enterobacteria from penetrating the internal organs. The irradiated preparations were more active than native ones.
Assuntos
Soros Imunes/efeitos da radiação , Lesões Experimentais por Radiação/prevenção & controle , Protetores contra Radiação , Animais , Cricetinae , Feminino , Cavalos/imunologia , Soros Imunes/farmacologia , Imunidade Inata/efeitos da radiação , Técnicas In Vitro , Masculino , Camundongos , Lesões Experimentais por Radiação/imunologia , RatosRESUMO
In studying the plasmocyte reactions in the regional lymph nodes of intact and irradiated mice immunized with native and irradiated globulin it was established that the preparation exposed to 60Co-gamma-rays exhibited an increased capacity of activating the plasma cell proliferation.
Assuntos
Plasmócitos/imunologia , Lesões Experimentais por Radiação/imunologia , Soroglobulinas/imunologia , Animais , Radioisótopos de Césio , Feminino , Raios gama , Camundongos , Desnaturação Proteica , Soroglobulinas/efeitos da radiaçãoAssuntos
Anticorpos Heterófilos/administração & dosagem , Lesões Experimentais por Radiação/terapia , Soroglobulinas/uso terapêutico , Doença Aguda , Animais , Autoanticorpos/administração & dosagem , Cães , Raios gama/efeitos adversos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Lesões Experimentais por Radiação/mortalidadeRESUMO
Experiments were conducted on 220 female mice weighing 20--24 g, with the use of 3 types of antigens (sheep erythrocytes, vaccines from the intestinal and paratyphoid bacilli). There proved to be an increase on the 7th and the 14th day of the formation of specific antibodies under the effect of subcutaneous injection of a homologous blood (0.1--0.3 ml per mouse) 2 hours after the antigen immunization. Hemostimulation not only intensified the antibody-genesis, but also increased the resistance to the infection with the living microbial culture. The stimulating action of the blood injection persisted in irradiation of the mice with gamma-rays in a dose of 300 r. Hemostimulation produced an activation of the normal autoantibody system capable of influencing the function of cells necessary for the antigen assimilation.