[Immunohistochemical study of cells of invasive cancer of the vaginal part of the cervix uteri]. / Immunogistokhimicheskoe issledovanie kletok invazivnogo raka vlagalishchnoi chasti sheiki matki.

Immunohistochemical study of cervical carcinoma used EE21-06d monoclonal antibodies which identify five cytokeratin polypeptides inherent in the squamous epithelium. PAP-method and reaction of immunofluorescence were employed. Initial stages of squamous cell carcinoma invasion were characterized by bleaching or complete cell discoloring of most tumor cells. However, in deeply invading tumors, the share of intensively stained cells was markedly increased. The results point to expression of different cytokeratins or cell clones replacement with tumor progression. The peculiarities of cytokeratin distribution may serve to determine the degree of invasion and differentiation of tumor cells.

[Cytokeratin expression in squamous-cell metaplasia of the cervix uteri: an immunohistochemical study using monoclonal antibodies]. / Ekspressiia tsitokeratinov pri ploskokletochnoi metaplazii sheiki matki: immunogistokhimicheskoe issledovanie s pomoshch'iu monoklonal'nykh antitel.

Immunohistochemical study of the exo- and endocervix in squamous metaplasia is performed using EE21-06d monoclonal antibodies revealing simultaneously five cytokeratinous polypeptides characteristic of squamous epithelium, and f-12-19h revealing simultaneously wide spectrum of cytokeratinous polypeptides characteristic of the epithelium various types. PAP method and the reaction of immunofluorescence were used. Monoclonal antibodies 12-19d label cells of all layers of normal exocervix, endocervix and metaplastic squamous epithelium, both immature and differentiated. Monoclonal antibodies EE21-06d react in the exocervix with suprabasal and higher located cells and in the endocervix with reserve cells only. Positive reaction with EE21-06d in the metaplastic foci increases in proportion with the squamous cell differentiation, thus this antibody being the marker of reserve cells and the degree of the squamous metaplasia maturity.

[Immunohistochemical identification of reserve cells of the endocervical canal by monoclonal antibodies EE21-06d]. / Immunohistokhimicheskaia identifikatsiia rezervnykh kletok éndotservikal'nogo kanala monoklonal'nymi antitelami EE21-06d.

Expression of cytokeratin polypeptides characteristic of squamous epithelium was studied in reserve cells of cervical canal obtained from 8 women by the immunofluorescence method with the help of monoclonal antibodies EE21-06d (MAB). MAB EE21-06d were shown to detect individual reserve cells as well as their hyperplasia foci without staining columnar cells.

[Lymphoid cell infiltration of malignant epithelial tumors in humans]. / Limfoidno-kletochnaia infil'tratsiia zlokachestvennykh épiteial'nykh opukholei cheloveka.

210 malignant tumors of different sites were studied by morphohistochemical, ultrastructural and stereometric means. Lymphoid cell infiltration was shown to be a morphologic manifestation of immune antitumor reactions. Neoplasms may show very high levels of lymphoid cell infiltration. However, the level of the said cells in the parenchyma and degree of cytotoxic effect on cancer cells are insufficient for antitumor effect to come about. Such factors as parenchyma/stroma ratio, degree of cell differentiation and stage of tumor may influence the level and pattern of infiltration.

[The effect of preparations of microbial glutamin(asparagin)ase on cultured tumor cells]. / Deistvie preparatov glutamin[asparagin]azy iz mikroorganizmov na opukholevye kletki v kul'ture.

Glutamin (asparagin)ase from Pseudomonas boreopolis 526 is shown to produce a cytotoxic effect on the human ovary carcinoma cells (CaOv line), Fisher lympholeukemia (L-8 line) and Burkitt's lymphoma cells (P3HR line). A significant inhibition of the DNA synthesis is found in L-8 and P3HR cell lines. The enzyme is less active in the cells of the CaOv line.

[Morphofunctional characteristics of male gonads in cancer of different localization]. / Morfofunktsional'nye osobennosti muzhskikh gonad pri rake razlichnoi lokalizatsii

Morphological and histochemical changes in the genital organs in 62 men, who had died of cancer of various localizations, and those in the testes in 65 practically healthy men aged 30-90 years, who had died of various causes, (controls), were studied. The studies conducted showed that changes in the male gonads in cancer of various localizations were similar to involutional ones, the former, however, were more pronounced and occurred in younger men. It was established that the functional activity of granulocytes in cancer dropped,whereas the activity of sustentocytes remained at a high level. This was one of the causes of impairment of the hormonal balance in the organism of a patient, cancer carrier, and of inhibition of the spermatogenesis activity. The degree and depth of changes depended on the stage and duration of the disease, localization of tumours was of no significance.