RESUMO
The lethality and cytokines-inducing activity of lipopolysaccharides (LPS) obtained from nodulating bacteria, Rhizobium leguminosarum and Mesorhizobium loti, were compared to those of Salmonella typhimurium LPS. The activity of R. leguminosarum LPS was almost comparable to Salmonella endotoxin in terms of lethality, Limulus lysate gelating activity and in vivo tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and interferon-gamma (IFN-gamma) induction capacity. In contrast to high lethal toxicity of Rhizobium LPS, the lethality of LPS isolated from Mesorhizobium loti was more than 10(3)-fold lower. Weak lethality of LPS from Mesorhizobium correlated with low capacity of this LPS to induce TNF-alpha, IL-1beta, IL-6 and IFN-gamma both in vivo and in vitro in murine splenocytes. The examined overall chemical composition of LPS indicates a considerable distinction in their lipid A regions. Lipid A's obtained from R. leguminosarum and M. loti differed from their enterobacterial counterpart with respect to lipid A sugar backbone, its phosphate content as well as the type and distribution of hydrophobic acyl residues. The relation of lipid A chemotype and bioactivity of LPS from the two Rhizobiaceae genera is discussed.
