RESUMO
The form in which radioactive caesium is present in muscular tissue has been investigated. It has been found that protein agents under study do not form stable complexes with radioactive caesium. It is suggested that caesium-137 is partially bound by muscular cell membranes upon meet storage.
Assuntos
Radioisótopos de Césio/análise , Músculos/química , Actomiosina/análise , Animais , Autorradiografia , Bovinos , Eletroforese em Gel de Poliacrilamida , Carne/análise , Espectrometria gamaRESUMO
The article deals with conditions for splitting the Penicillium vitale glucosooxidase molecule into apoenzyme and coenzyme, for reconstruction of the enzyme from apoenzyme and FAD as well as for the effect of some factors ("labilizing" fraction, dithiotreitol) on it. Specific activity of the reconstructed enzyme is on the average 85% of the initial enzyme specific activity: "the labilizing" fraction inhibits reconstruction of glucosooxidase from apoenzyme and FAD, that is due to oxidation of the apoenzyme sulphydryl groups. Possible participation of sulphydryl groups in adding FAD to apoenzyme is under discussion.