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2.
Klin Med (Mosk) ; 90(4): 18-22, 2012.
Artigo em Russo | MEDLINE | ID: mdl-22896974

RESUMO

Hepatocyte transplantation represents a supplementary strategy for treating liver diseases. Several methods including extracorporeal devices, cell transplantation and implanted tissue-engineered units were proposed as liver support before liver transplantation. The ability to repopulate the liver with healthy and disease-resistant hepatocytes opens up new possibilities for correcting genetic disorders and treating patients with chronic liver diseases. Some results of experimental and clinical therapy of liver diseases are summarized in this review.


Assuntos
Insuficiência Hepática/cirurgia , Hepatócitos/transplante , Doença Crônica , Insuficiência Hepática/genética , Humanos
3.
Biomed Khim ; 57(5): 535-43, 2011.
Artigo em Russo | MEDLINE | ID: mdl-22629604

RESUMO

The cultivation of multipotent mesenchymal stromal bone marrow cells and cells of A-431, MDCK, Vero, 3T3 and Hep-G2 was performed on polymeric films (PVA) with different hydrophobic fatty acid residues. The cells of different types grew on these films with different intensity, but in the most cases comparable with the cultivation control on usual plastic. The examined films were nontoxic to cells and sufficiently adhesive. They did not changed pH of cultural media, were optically transparent under microscope and comfortable in the experimental work. These films can be used as a model for the artificial organ construction. The covalent binding of different fatty acids to PVA shows possibility of the adaptable changes of films properties (hydrophobity and adhesiveness), and therefore possibility of the creation of optimal conditions for different cell types attachement and growth.


Assuntos
Células da Medula Óssea/citologia , Técnicas de Cultura de Células , Álcool de Polivinil/química , Engenharia Tecidual/métodos , Células 3T3 , Animais , Bovinos , Proliferação de Células , Sobrevivência Celular , Chlorocebus aethiops , Meios de Cultura/química , Ácidos Graxos/química , Haplorrinos , Células Hep G2 , Humanos , Camundongos , Ratos , Células-Tronco/citologia , Células Vero
4.
Biomed Khim ; 56(4): 425-42, 2010.
Artigo em Russo | MEDLINE | ID: mdl-21032894

RESUMO

The liver transplantation is the most effective method for treating severe liver disease. The hepatocytes transplantation may serve as the perspective means for treating liver failure. This review analyzes the experimental approaches and perspectives on the adult hepatocytes use for the creation of implanting bioartificial liver module for hepatic failure treatment.


Assuntos
Bioprótese , Insuficiência Hepática/terapia , Hepatócitos/transplante , Animais , Técnicas de Cultura de Células , Ciclo Celular , Técnicas de Cocultura , Meios de Cultura , Matriz Extracelular , Terapia Genética , Hepatectomia , Hepatócitos/citologia , Humanos , Polímeros
5.
Biomed Khim ; 54(4): 376-91, 2008.
Artigo em Russo | MEDLINE | ID: mdl-18988455

RESUMO

The insufficiency of liver functions remains one of the major causes of death. The liver transplantation is the most effective method for treating severe liver diseases. The shortage of donor organs and high risk of graft rejection are the main problems for liver transplantation. Stem cells and isolated hepatocytes are alternative means for repopulating the liver after various injuries instead of liver transplantation. This review analyses the experimental and clinical advantage and perspectives on the stem cells use in clinical trials for treating hepatic failure.


Assuntos
Hepatócitos/citologia , Falência Hepática/terapia , Transplante de Células-Tronco/métodos , Células-Tronco/citologia , Animais , Ensaios Clínicos como Assunto , Hepatócitos/fisiologia , Hepatócitos/transplante , Humanos , Transplante de Fígado , Transplante de Células-Tronco/tendências , Células-Tronco/fisiologia
6.
Biomed Khim ; 51(5): 453-72, 2005.
Artigo em Russo | MEDLINE | ID: mdl-16342662

RESUMO

The therapeutic potential of gene and cell therapy in cardiovascular and systemic disease such as myocardial ishemia, postangioplasty restenosis, hypertensia, arteriosclerosis, trombogenicity, posttransplantation complications is considered.


Assuntos
Doenças Cardiovasculares/terapia , Transplante de Células , Terapia Genética , Animais , Modelos Animais de Doenças , Transplante de Coração , Transplante de Fígado
7.
Bull Exp Biol Med ; 140(1): 127-31, 2005 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16254638

RESUMO

Original recombinant adenoviral construction carrying E. coli beta-galactosidase LacZ gene designed by the authors is convenient for labeling and monitoring of bone marrow mesenchymal (stromal) progenitor cells and myocardial and skin fetal cells transplanted in damaged rat tissues (in the perinecrotic zone of the myocardium and onto burnt skin surface) for their reparation. This genetic construction after pre-inactivation of endogenous beta-galactosidase allows to detect transplanted cells in the foci of injury; positive effects of transplantation on tissue reparation processes can be attributed to the presence of transplanted cells.


Assuntos
Técnicas de Transferência de Genes , Vetores Genéticos/genética , Transplante de Células-Tronco Mesenquimais/métodos , Células-Tronco Mesenquimais/citologia , Adenoviridae , Animais , Escherichia coli , Técnicas Histológicas , Óperon Lac/genética , Ratos , beta-Galactosidase/genética
8.
Biomed Khim ; 50(1): 3-24, 2004.
Artigo em Russo | MEDLINE | ID: mdl-15108623

RESUMO

Review considers perspectives and the main advantages in the development of the approaches to gene therapy in organ and cell transplantology. The methods of prevention in allo- and xenotransplantation by the inhibition of the recipient immune system, and the problem of specific tolerance to graft in host organism creation by mixed antigenic chimerism making by means of gene therapy methods are discussed.


Assuntos
Terapia Genética , Rejeição de Enxerto/prevenção & controle , Transplante de Órgãos/métodos , Animais , Técnicas de Transferência de Genes , Rejeição de Enxerto/imunologia , Humanos , Transplante Heterólogo/imunologia , Transplante Homólogo/imunologia
11.
Ter Arkh ; 70(4): 45-8, 1998.
Artigo em Russo | MEDLINE | ID: mdl-9612903

RESUMO

AIM: Investigation of potentialities of polymerase chain reaction (PCR) in diagnosis of cytomegalovirus infection in recepients of allotransplants. MATERIALS AND METHODS: PCR with primers on the MIE region was used to examine the blood of recepients of the kidney, heart, hemodialysis patients, donors (199, 6, 35 and 67 subjects, respectively) for DNA of cytomegalovirus (CMV). RESULTS: CMV DNA was found in none of the donors, 95 recepients. Severity of clinical symptoms in blood carriers of CMV correlated with the level of DNA CMV (p < 0.001). 85% of those with high levels of DNA CMV (1000 per 10(5) leukocytes) had acute clinical symptoms. 15.8% of all the PCR-positive patients had no symptoms at the time of the examination. Specific antivirus ganciclovir therapy was given to 50% of PCR-positive patients and to 85% of those who had DNA CMV higher than 1000 c. per 10(5) leukocytes. The treatment resulted in a sharp fall or disappearance of DNA CMV and symptoms of CMV disease. CONCLUSION: PCR can be applied in diagnosis of CMV infection in allotransplants recepients.


Assuntos
Infecções por Citomegalovirus/diagnóstico , Citomegalovirus/genética , DNA Viral/análise , Transplante de Coração/efeitos adversos , Transplante de Rim/efeitos adversos , Reação em Cadeia da Polimerase , Anticorpos Antivirais/análise , Antivirais/uso terapêutico , Citomegalovirus/imunologia , Infecções por Citomegalovirus/tratamento farmacológico , Infecções por Citomegalovirus/etiologia , Ganciclovir/uso terapêutico , Humanos , Técnicas Imunoenzimáticas , Leucócitos/virologia , Diálise Renal , Sensibilidade e Especificidade , Doadores de Tecidos , Transplante Homólogo
12.
Klin Lab Diagn ; (11): 19-20, 33-4, 1998 Nov.
Artigo em Russo | MEDLINE | ID: mdl-10205654

RESUMO

Ninety-eight patients with organ allografts were examined using the polymerase chain reaction (PCR). Cytomegalovirus (CMV) DNA was not found in the blood of any of 67 blood donors. At least 85% patients with CMV DNA in the plasma developed CMV disease. CMV DNA was detected in leukocytes of 22 (40.7%) out of 54 patients with negative results of analysis for CMV DNA in the plasma; 16 (29.6%) of these 22 presented with clinical symptoms of CMV infection by the moment of investigation. Results of PCR analysis of blood, urine, and saliva specimens of 30 patients examined did not always coincide. DNA isolated from the urine can contain components inhibiting DNA polymerase. Blood leukocytes or whole blood are the most suitable for the diagnosis of active and symptomatic CMV-infection.


Assuntos
Citomegalovirus/genética , DNA Viral/sangue , Transplante de Coração , Transplante de Rim , Doadores de Sangue , Infecções por Citomegalovirus/diagnóstico , Humanos , Leucócitos/virologia , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/estatística & dados numéricos , Complicações Pós-Operatórias/diagnóstico , Sensibilidade e Especificidade , Transplante Homólogo
13.
Artigo em Russo | MEDLINE | ID: mdl-7520657

RESUMO

In this work an antigen obtained from the culture fluid of C. tropicalis strain 928 is described. The strain was cultivated in a synthetic culture medium in fermenters. The culture fluid was concentrated in cartridges and then on membranes. The concentrate was electrophoretically separated in an agar block. The isolated fraction was purified on Con-A Sepharose. The antigen thus obtained proved to be homogeneous in immunophoresis and in immunodiffusion with homologous serum to the culture fluid. In immunodiffusion and immunophoresis this purified fraction did not precipitate with sera to antigens obtained from the culture fluids of strains of other Candida species.


Assuntos
Antígenos de Fungos/isolamento & purificação , Candida/imunologia , Epitopos/isolamento & purificação , Animais , Antígenos de Fungos/análise , Antígenos de Fungos/imunologia , Meios de Cultura , Relação Dose-Resposta Imunológica , Epitopos/análise , Epitopos/imunologia , Imunização , Imunodifusão , Imunoeletroforese , Coelhos , Especificidade da Espécie , Fatores de Tempo
14.
Gig Tr Prof Zabol ; (3): 33-4, 1991.
Artigo em Russo | MEDLINE | ID: mdl-1879735

RESUMO

The membrane filtration technique and column gel chromatography+ (with sepharose 6B or sephacryl S-200) were used to receive fractions of KJ Candida maltosa, strain VSB 899, which were studied with highly effective liquid chromatography, immunochemical reactions and intracutaneous allergotesting on guinea-pigs sensibilized with VSB 899 live cultures. In the whole KJ strain VSB 899 concentrate, 8 components were identified, six of which had molecular masses from 350,000 to 18,000 and had no analogue in the surface antigen preparation (SAP) from the same strain of yeast-like fungi. It was shown that the SAP-related antigen determinants were present in predominantly polysaccharide-containing fractions. In intracutaneous allergotesting, chromatographic fractions with molecular masses of 50,000 and 18,000-35,000 were most manifesting. It was supposed that still further purification of the allergen will increase its specificity.


Assuntos
Alérgenos/isolamento & purificação , Antígenos de Fungos/isolamento & purificação , Candida/imunologia , Candidíase/diagnóstico , Microbiologia Industrial/métodos , Doenças Profissionais/diagnóstico , Medicina do Trabalho/métodos , Animais , Candidíase/imunologia , Cromatografia em Gel/métodos , Meios de Cultura , Cobaias , Humanos , Doenças Profissionais/imunologia
16.
Zh Mikrobiol Epidemiol Immunobiol ; (11): 71-5, 1981 Nov.
Artigo em Russo | MEDLINE | ID: mdl-6275640

RESUMO

Guinea-pigs were immunized with anatheta-hemolysin preparations adsorbed on aluminum hydroxide, as well as with the mixture of anatheta-hemolysin and type A Cl. perfringens toxoid, purified and concentrated. Anatheta hemolysin preparations were obtained with the use of homogeneous theta hemolysin, as well theta hemolysin of various purification degrees. As a result, antatheta hemolytic guinea-pig sera capable of neutralizing 2,000-8,000 HU of theta-hemolysin were obtained. Tests made to establish the degree of protection in the immunized guinea-pigs did not show that the animals immunized with the mixture of anatheta-hemolysin and type A Cl. perfringens toxoid, purified and concentrated, had any advantages in the degree of protection over the animals immunized with the toxoid alone. But there is no doubt that this component plays a positive role under the conditions of natural gas gangrene when the hemolytic action of Cl. perfringens toxin becomes considerably pronounced.


Assuntos
Clostridium perfringens/imunologia , Imunização , Animais , Cobaias , Hemólise , Soros Imunes , Toxoides/imunologia
17.
Vopr Med Khim ; 27(1): 129-32, 1981.
Artigo em Russo | MEDLINE | ID: mdl-6258318

RESUMO

Teta-hemolysine was purified from Cl. perfringens strain BP6K 28 as follows: reprecipitation in the isoelectric point of the enzyme with 2 N H2SO4 containing 15% NaCl, DEAE cellulose chromatography, gel filtration on Sephadex G-100-G75 or Bio Gel P-60--P-100, rechromatography on DEAE-Sephadex A-50 and affinity chromatography. The biological activity of homogenous teta-hemolysine, estimated by complete hemolysis of human erythrocytes, exceeded 100, 000 theta E over mg protein but the enzyme was highly labile. Molecular weight of the enzyme was 53,000 daltons.


Assuntos
Clostridium perfringens/análise , Proteínas Hemolisinas/isolamento & purificação , Cromatografia de Afinidade , Cromatografia em Gel , Cromatografia por Troca Iônica , Ponto Isoelétrico , Peso Molecular
18.
Vopr Med Khim ; 21(2): 143-7, 1975.
Artigo em Russo | MEDLINE | ID: mdl-237365

RESUMO

Five strains of A type Cl. perfringens of different titres of toxicity (BP6K, SR-12, 2836, 2910, 1) possessed a distinct activity of ornithine transcarbamylase. In cells the maximal activity of the enzyme was observed within 3-5 hrs of growth of the culture. The enzyme was partially purified and some of its physico-chemical properties were studied. Ornithine transcarbamylase was termostable. Monoiodine acetate, p-chloromercurybenzoate, semicarbazide, Hg-2+, Cu-2+ and Fe-3+ inhibited the enzymatic activity, but Mg-2+ and Ca-2+ activated the enzyme. Ornithine transcarbamylase was shown to be active in wide region of pH: from pH 6.0 to pH 9.0 and higher. Two peaks of the enzyme activity were observed: the first (a more distinct one) at pH 8.6-8.9 and the second, smaller,--at pH 6.5-6.7.


Assuntos
Clostridium perfringens/enzimologia , Ornitina Carbamoiltransferase/metabolismo , Fracionamento Celular , Cromatografia DEAE-Celulose , Cromatografia em Gel , Citrulina/biossíntese , Clostridium perfringens/patogenicidade , Meios de Cultura , Estabilidade de Medicamentos , Ativação Enzimática/efeitos dos fármacos , Repressão Enzimática/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Ornitina Carbamoiltransferase/antagonistas & inibidores , Ornitina Carbamoiltransferase/isolamento & purificação , Temperatura , Fatores de Tempo , Ultrassom
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