Assuntos
Queimaduras/tratamento farmacológico , Inibidores de Proteases/uso terapêutico , Sepse/tratamento farmacológico , Toxemia/tratamento farmacológico , Cicatrização/efeitos dos fármacos , Adolescente , Adulto , Aprotinina/administração & dosagem , Coagulação Sanguínea/efeitos dos fármacos , Tecido de Granulação/efeitos dos fármacos , Humanos , Pessoa de Meia-Idade , Inibidores da Tripsina/administração & dosagemAssuntos
Queimaduras/metabolismo , Nutrição Parenteral , Proteínas/metabolismo , Adolescente , Adulto , Idoso , Queimaduras/terapia , Humanos , Pessoa de Meia-IdadeAssuntos
Curativos Biológicos , Queimaduras/cirurgia , Adolescente , Adulto , Idoso , Superfície Corporal , Humanos , MasculinoAssuntos
Bandagens , Queimaduras/terapia , Polímeros , Animais , Estudos de Avaliação como Assunto , Humanos , Coelhos , CicatrizaçãoRESUMO
Activities of proteolytic enzymes--cathepsins B and D, trypsin-like proteases, leucine aminopeptidase--as well as of lactate dehydrogenase and its isoenzymes were studied in area of thermic burns of skin and hypodermic tissue, in the zone surrounding the burns area and in intact skin of burned rats. Within a day after burns activities of the enzymes studied were distinctly decreased. The low levels of the activities were within the next three weeks. The activities of the proteolytic enzymes were gradually restored and exceeded the normal level in the tissues situated under crust, in boundary zone and in leukocytes of demarcational zone; the increase in activity appears to affect the subsequent deepening of burn wounds.
Assuntos
Queimaduras/enzimologia , L-Lactato Desidrogenase/metabolismo , Peptídeo Hidrolases/metabolismo , Monoéster Fosfórico Hidrolases/metabolismo , Pele/enzimologia , Fosfatase Ácida/metabolismo , Adenosina Trifosfatases/metabolismo , Fosfatase Alcalina/metabolismo , Animais , Catepsinas/metabolismo , Isoenzimas , Leucil Aminopeptidase/metabolismo , Masculino , Ratos , Tripsina/metabolismoRESUMO
The effect of proteolytic inhibitor contrical on the experimental burn wound healing was studied in rats using biochemical, histological and histochemical methods. In control untreated animals with flame burn of 20% of body surface wound healing was associated with development of secondary necrosis, marked inflammatory reaction, augmented activity of proteases and peptidases. The use of contrical prevented secondary necrosis; this effect was apparently related to reduction of tissue proteolytic enzymes activity.
Assuntos
Aprotinina/uso terapêutico , Queimaduras/tratamento farmacológico , Pele/lesões , Fosfatase Ácida/metabolismo , Fosfatase Alcalina/metabolismo , Animais , Queimaduras/enzimologia , Masculino , Necrose/prevenção & controle , Peptídeo Hidrolases/metabolismo , Ratos , Pele/enzimologia , CicatrizaçãoRESUMO
Effect of a polyvalent inhibitor of proteinases from bovine lungs (industrial preparation -- ingitrile, commercial grade -- conntrical) on the state of kinin system was studied in blood serum of patients with extensive burns at shock periods and in acute burn toxemia. Decrease in content of kallikreinogene, which is typical for shock and acute, toxemia and which reflects the activation of kallikrein-kinin system, was less distinct in patients, treated with the inhibitor, than in patients, which were not treated with ingitrile. The early and rapid restoration in kininogene content and distinct inhibition of the total arginine-esterase activity were observed in blood serum after treatment with the inhibitor. The inhibitor did not affect the phase alterations in the carboxypeptidase N activity within the first 3 days after the burns; the distinct decrease in the enzymatic activity was confirmed within the first period of the burn shock. Within 24-48 hrs after the burns content of alpha1-antitrypsin was distinctly increased in patients treated with ingitrile as compared with the untreated group. The data obtained suggest that the polyvalent proteinase inhibitor causes a decrease in activity of the kallikreinkinin system in blood plasma and affects the enzymes participating indirectly in formation of kinins in burned patients. The data obtained are in agreement with the distinct clinical effect of the inhibitor. The doses of the inhibitor used in these studies did not cause normalizing effect on the activity of the kinin system components and on the clinical state of patients only in extremely severe cases of burn shock and in acute burn toxemia with letal outcome within the few days after the injury.