Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Biotechnol ; 251: 186-188, 2017 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-28359866

RESUMO

Light-up aptamers are practical tools to image RNA localization in vivo. A now classical light-up aptamer system is the combination of the 3,5-difluoro-4-hydroxybenzylidene (DFHBI) fluorogen and the RNA aptamer Spinach, which has been successfully used in bacterial and mammalian cells. However, light-up aptamers have not been used in algae. Here, we show that a simple vector, carrying Spinach, transcriptionally fused to the aphA-6 gene, can be effectively used to generate a functional light-up aptamer in the chloroplast of Chlamydomonas reinhardtii. After incubation with DFHBI, lines expressing the aphA-6/Spinach mRNA were observed with laser confocal microscopy to evaluate the functionality of the light-up aptamer in the chloroplast of C. reinhardtii. Clear and strong fluorescence was localized to the chloroplast, in the form of discrete spots. There was no background fluorescence in the strain lacking Spinach. Light-up aptamers could be further engineered to image RNA or to develop genetically encoded biosensors in algae.


Assuntos
Aptâmeros de Nucleotídeos/genética , Chlamydomonas reinhardtii/genética , Cloroplastos/genética , Compostos de Benzil , Fluorescência , Corantes Fluorescentes , Imidazolinas , Canamicina Quinase/genética , RNA Mensageiro/genética , RNA de Plantas/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...