Teeth as an Indicator of the Environmental Exposure of Silesia Province's Inhabitants in Poland to Metallic Trace Elements.

(1) Background: The elemental composition of teeth can provide an estimate of environmental exposure to heavy metals. The aim of this study was to analyze the possibility of using teeth in the biomonitoring of environmental exposure to heavy metals as an indicator of contaminants present in the human residential environment. (2) Methods: The research materials were 110 samples of extracted teeth. The samples were taken from people living in three areas in the province of Silesia. The concentrations of cadmium, lead, and mercury in the samples were determined. (3) Results: The results of the chemical analysis of the collected samples showed a significant variation in the concentrations of heavy metals (Cd, Pb, and Hg) in the analyzed teeth. Furthermore, the mean concentrations of the analyzed heavy metals in the teeth varied according to the patient's place of residence, the type of tooth analyzed, the presence of caries in the patient, and the smoking or non-smoking status of the patient. (4) Conclusions: The results of the chemical analysis of the teeth of inhabitants of three cities in the most polluted region of Poland indicate that they can be used as an indicator of environmental exposure to cadmium, lead, and mercury.

Type D Personality and Health Behaviors in People Living with Obesity.

BACKGROUND: Considering that health behaviors and personality traits play an important role in the formation of health attitudes, the main objective of this study was to evaluate the relations that occur between type D personality and health behaviors in a group of obese patients. METHODS: 443 adult patients with BMI ≥ 30 kg/m2, who had been hospitalized in selected hospital facilities in the Silesian Voivodeship (Poland), participated in the study. Respondents completed three standardized questionnaires-the Multidimensional Health Locus of Control Scale, version A (MHLC-A), the Inventory of Health Behaviors (IZZ), and the Type D Scale (DS-14). RESULTS: Patients with type D personality were characterized by the least effective mental attitudes and preventive behaviors, and differed significantly from the other personality types (intermediate and non-type D). Type D personality increased the risk of initiating improper health behaviors by more than five times. Regarding the sense of health control, patients with type D personality had significantly lower scores for the Internal Dimension subscale (21.3 ± 3.1) and higher for the Powerful Others Dimension subscale (24.0 ± 2.6), compared to patients with intermediate and non-type D personality. Proper health behaviors correlated with an internal sense of health control; the strongest correlation, defined as a medium, was with Preventive Behaviors (R = 0.42; p < 0.0001). CONCLUSIONS: Type D personality was associated with poorer attitudes towards health. Among obese respondents with a type D personality, there was a significantly higher prevalence of those who believed that their health status was a consequence of chance events.

Personality Type D, Level of Perceived Stress, Insomnia, and Depression Among High School Teachers in Poland.

Teaching is inherently connected with specific burdens that may imply stressful situations. The goal of this study was to explore the prevalence of type D (distressed) personality in teachers. This is known to cause depressive episodes and sleep disorders, which not only have direct physical health effects, but can also impact the wellbeing of individuals and hence adversely affect their job performance. The participants consisted of 412 high school teachers from the Silesian Province, located in the south of Poland. Using the following research tools: Type D Scale (DS14), Perceived Stress Scale, Athens Insomnia Scale, and Beck Depression Inventory, it was found that type D personality was observed in a large percentage of teachers (30.1%). It was reported that teachers with distressed personality suffered from insomnia and depression significantly more often. Findings from the current study indicate the need to implement preventive activities focused on reducing psychosocial risk factors in the work environment in order to reduce the frequency of depressive disorders among teachers.

Concentration of cadmium and lead in vegetables and fruits.

Chemical contamination of foods pose a significant risk to consumers. A source of this risk is due to the consumption of products contaminated with heavy metals such as cadmium (Cd) and lead (Pb). The aim of the study was to research the levels of Cd and Pb contamination of selected species of vegetables and fruits in the form of fresh, frozen, dried and processed products. The goal was to verify which of these food groups was more contaminated with heavy metals. The study covered 370 samples of fruits and vegetables including apples, pears, grapes, raspberries, strawberries, cranberries, as well as beetroots, celeries, carrots and tomatoes. The content of Cd and Pb was determined by atomic absorption spectrometry. Quantitative results were analyzed using statistical models: analysis of variance, outlier analysis, post-hoc multiple comparison Tukey test. The tests showed that the levels of Cd and Pb concentration in samples of fresh, processed, frozen and dried fruits and vegetables varied substantially. The highest concentrations were recorded in dried products. Several fruit and vegetable samples exceeded the maximum permissible concentrations of Cd and Pb. The contamination of these products could be a significant source of consumer exposure to heavy metals when these products are a part of the diet.

The relationship between children's non-nutrient exposure to cadmium, lead and zinc and the location of recreational areas - Based on the Upper Silesia region case (Poland).

INTRODUCTION: The aim of the study was to identify the places that could pose a threat to children's health due to the significant concentration of heavy metals in the top layer of soil on which they actively spend their time. MATERIALS AND METHODS: The research material consisted of 144 topsoil samples from 10 randomly selected playgrounds and 10 sports fields located in two cities in Poland. The content of cadmium (Cd), lead (Pb) and zinc (Zn) in the analyzed samples was determined by atomic absorption using the INTEGRA XL ICP spectrometer with inductively coupled plasma. RESULTS: From the 144 collected soil samples 48 (33%) exceeded the normative value for at least one of the three analyzed heavy metals. The maximum permissible concentration of all three elements was exceeded in the case of 15 samples. The heavy metals concentrations determined in the soil samples taken from the sports fields and playgrounds were in the following ranges, respectively: 0.3-43.0 mg/kg d. w. - Cd; 1.3-1393.8 mg/kg d. w.- Pb; 12.5-4315.8 mg/kg d. w. - Zn. Estimated children's exposure to heavy metals (Cd and Pb) via non-nutrient route indicates a significant risk which can be a place of recreation of the youngest children, as a result of secondary dusting during activity and games. CONCLUSIONS: In one-third of the analyzed soil samples from playgrounds and sport fields significant contamination with heavy metals (Cd, Pb, Zn) was indicated. In extreme cases, the maximum permissible concentration was exceeded over 20-fold, constituting an important risk factor for children's health.

[Comparison of exposure to stress and analysis of ways of coping with stress among freight transport and public transport drivers]. / Porównanie narazenia na stres i analiza sposobów radzenia sobie z nim wsród kierowców samochodów dostawczych i kierowców komunikacji miejskiej.

BACKGROUND: Fast progress in a lot of economic sectors has greatly contributed to a growing role of road transportation systems, including freight transport and passenger transport. The job of professional drivers is regarded as extremely hard and dangerous, it is associated with high risk of health loss and even life loss. This profession is also associated with mental burden, the main cause of the absence at work and alarming number of road accidents. The aim of study was to compare exposure to stress, check the level of stress and ways to cope with stress in 2 groups of drivers (N = 187). MATERIAL AND METHODS: The study was carried out among public transport drivers and freight transport drivers. The authors' own questionnaire and 2 psychological tests: Perceived Stress Scale (PSS-10) and Posttraumatic Growth Inventory and Inventory to Measure Coping Strategies with Stress (Mini-COPE) were used as the study tools. RESULTS: The level of stress is high in both groups, mostly due to a similar type of work. Both groups practice similar ways to cope with stress, but active ways predominate. CONCLUSIONS: The work of a professional driver is considered as extremely stressful. The level of stress among professional drivers should be under continuous control. Employers should introduce preventive programs and educate employees about some professional ways to cope with stress. Med Pr 2016;67(4):455-466.

16-Membered Macrolide Lactone Derivatives Bearing a Triazole-Functionalized Arm at the Aglycone C13 Position as Antibacterial and Anticancer Agents.

A series of new C13-triazole-bridged and C13-ether leucomycin analogues with a reduced aldehyde group were synthesized. Derivatives with the highest antibacterial [MIC values (S. epidermidis, S. pneumoniae): ∼2-4 µg mL(-1) ; 2.55-5.09 µm] and cytotoxic [IC50 values (HeLa, KB, MCF-7, A549, HepG2 cells): ∼1.35-3.70 µm] potencies were those with the best aqueous solubility and bearing a saccharide-triazole arm at the C13 position of the aglycone. These derivatives preferentially bind at the ribosomal tunnel and show the most attractive selectivity indexes [SI; calculated relative to the human dermal fibroblast (HDF) cell line], even higher than that of the reference compound cytarabine. Results of molecular docking studies of this type of macrolide antibiotics at the ribosomal tunnel, together with experimentally determined lipophilicity and aqueous solubility values, as well as biological assay data revealed the importance of the introduced functional group at the aglycone C13 arm to the future design of anticancer and antibacterial drug candidates. Our results clearly indicate that the high antibacterial and anticancer activities of these types of macrolides do not necessarily depend on the presence of the aldehyde group at the aglycone lactone ring.

Structure-activity relationship studies of new rifamycins containing l-amino acid esters as inhibitors of bacterial RNA polymerases.

New rifamycins (1-12) combined with different l-amino acids, containing methyl, ethyl, tert-butyl and benzyl groups at the ester part, via amine linkage, were synthesized and their structures in solution were determined by spectroscopic FT-IR and 1D and 2D NMR methods as well as visualized by DFT calculations. Two types of rifamycin structures were detected in solution: a zwitterionic one with the transferred proton from O(8)H phenol to secondary N(38) atom and a pseudocyclic structure stabilized via formation of intramolecular H-bond within the protonated basic C(3)-substituent. The presence of these rifamycins' structures influenced physico-chemical (logP, solubility) parameters and antibacterial properties. The bulkiness at the ester substituent of new rifamycins containing aromatic l-amino acids was found to be an important factor, besides the solubility, to achieve relatively high antibacterial activity against reference S. epidermidis and reference S. aureus and MRSA strains (MICs 0.016-0.063 µg/mL), comparable to that of rifampicin. SAR for the novel derivatives was discussed in view of the calculated structures of rifamycin-RNAP complexes.

Structure and evaluation of antibacterial and antitubercular properties of new basic and heterocyclic 3-formylrifamycin SV derivatives obtained via 'click chemistry' approach.

Thirty four novel derivatives of 3-formylrifamycin SV were synthesized via reductive alkylation and copper(I)-catalysed azide-alkyne cycloaddition. According to the obtained results, 'click chemistry' can be successfully applied for modification of structurally complex antibiotics such as rifamycins, with the formation of desired 1,2,3-triazole products. However, when azide-alkyne cycloaddition on 3-formylrifamycin SV derivatives demanded higher amount of catalyst, lower temperature and longer reaction time because of the high volatility of substrates, an unexpected intramolecular condensation with the formation of 3,4-dihydrobenzo[g]quinazoline heterocyclic system took place. Structures of new derivatives in solution were determined using one- and two-dimensional NMR methods and FT-IR spectroscopy. Computational DFT and PM6 methods were employed to correlate their conformation and acid-base properties to biological activity and establish SAR of the novel compounds. Microbiological, physico-chemical (logP, solubility) and structural studies of newly synthesised rifamycins indicated that for the presence of relatively high antibacterial (MIC ~0.01 nmol/mL) and antitubercular (MIC ~0.006 nmol/mL) activities, a rigid and basic substituent at C(3) arm, containing a protonated nitrogen atom "open" toward intermolecular interactions, is required.