A taxonomic backbone for the Plumbaginaceae (Caryophyllales).

A taxonomic backbone of the Plumbaginaceae is presented and the current state of knowledge on phylogenetic relationships and taxon limits is reviewed as a basis for the accepted taxon concepts. In total, 4,476 scientific names and designations are treated of which 30 are not in the family Plumbaginaceae. The Plumbaginaceae are subdivided in three tribes with 26 genera and 1,179 accepted species. Two subgenera, 17 sections, two subsections and 187 infraspecific taxa are accepted. At the species and infraspecific level 2,782 synonyms were assigned to accepted taxa, whereas 194 names were excluded from the core checklist (i.e., unplaced taxa, infrageneric subdivisions with still uncertain application, names of verified uncertain application, invalid horticultural names, excluded names from other families, other excluded designations, and unresolved names). The EDIT Platform for Cybertaxonomy was utilized as the tool to compile and manage the names and further taxonomic data under explicit taxon concepts. Secundum references are given in case taxon concepts were taken from the literature, whereas this study serves as reference for newly circumscribed taxa. The family's division into the tribes Aegialitideae, Limonieae, and Plumbagineae departs from earlier two-subfamily classifications, prompted by recent phylogenetic findings that challenge the subfamilial affinity of Aegialitis. The genus Acantholimon was extended to include Gladiolimon, as currently available phylogenetic and morphological data support this merger. In Limonium, all accepted species could be assigned to sections and subsections or the "Mediterranean lineage", respectively, making use of the phylogenetic distribution of their morphological characters and states. A new combination and/or status is proposed for Dyerophytumsocotranum, Limoniumthymoides, Limonium×fraternum, Limonium×rossmaessleri, and Limoniumsect.Jovibarba. Special attention is given to nomenclatural issues, particularly for Staticenomenambiguum to resolve the names under accepted names. The use of artificial groupings like "aggregates", "complexes" and "species groups" in alpha-taxonomic treatments is discussed. The taxonomic backbone will receive continued updates and through the Caryophyllales Taxonomic Expert Network, it contributes the treatment of the Plumbaginaceae for the World Flora Online.

Anthriscus sylvestris-Noxious Weed or Sustainable Source of Bioactive Lignans?

Anthriscus sylvestris (L.) Hoffm. (Apiaceae), commonly known as wild chervil, has gained scientific interest owing to its diverse phytochemical profile and potential therapeutic applications. The plant, despite being categorized as a noxious weed, is traditionally used in treating various conditions like headaches, dressing wounds, and as a tonic, antitussive, antipyretic, analgesic, and diuretic. Its pharmacological importance stems from containing diverse bioactive lignans, especially aryltetralins and dibenzylbutyrolactones. One of the main compounds of A. sylvestris, deoxypodophyllotoxin, among its wide-ranging effects, including antitumor, antiproliferative, antiplatelet aggregation, antiviral, anti-inflammatory, and insecticidal properties, serves as a pivotal precursor to epipodophyllotoxin, crucial in the semisynthesis of cytostatic agents like etoposide and teniposide. The main starting compound for these anticancer medicines was podophyllotoxin, intensively isolated from Sinopodophyllum hexandrum, now listed as an endangered species due to overexploitation. Since new species are being investigated as potential sources, A. sylvestris emerges as a highly promising candidate owing to its abundant lignan content. This review summarizes the current knowledge on A. sylvestris, investigating its biological and morphological characteristics, and pharmacological properties. Emphasizing the biological activities and structure-activity relationship, this review underscores its therapeutic potential, thus encouraging further exploration and utilization of this valuable plant resource.

Main Poisonous and Allergenic Plant Species in Sicilian Gardens and Parks: Applications and Recommendations for Use.

This study identified the most common poisonous and allergenic plants occurring in Sicilian gardens and parks. Based on a survey conducted at 100 sites, a list was drawn up that reports the main biological and toxicological characteristics and ornamental uses of these plants. A total of 137 taxa were recorded, of which 108 were poisonous and 32 were allergenic. The most represented families were the Solanaceae, Moraceae, Apocynaceae and Fabaceae. The most represented geographical contingents were the European and the Mediterranean. A large number of toxic and allergenic plants recorded in Sicilian parks and gardens cause gastrointestinal disorders, 21 of which are deadly poisonous. Based on the results, actions for the management of existing gardens and the construction of new ones are discussed. The importance of environmental education for the population starting from school age is stressed. These recommendations aim to preserve cultivated biodiversity and, at the same time, protect human and pet health.

New Alien Plant Taxa for Italy and Europe: An Update.

Despite the wide amount of scientific contributions published on alien plant species, their diffusion dynamics, and their interactions with native taxa, it is increasingly difficult to slow down their spreading and their negative impact on habitats. Last recent years, in fact, a sharp rise in the number of new alien plant taxa introduced in Italy and Europe has been recorded. The aim of this work is to investigate most of the Italian territory in order to verify whether this alarming trend is still underway. Specimen collections and/or observations of alien plants have been performed in as many as 12 Italian regions. All the collected specimens are stored in public or private herbaria. Taxa have been identified according to the literature from the countries of origin of the investigated taxa, while the nomenclature followed the current international references. Updates on 106 taxa are reported. In particular, among 117 new records, 89 are first records, 27 are changes to status and there is 1 extinction. Seven new taxa for Italian alien flora are reported, two of which are new to Europe. The administrative regions with the highest number of records are Calabria (48), Sardegna (17) and Sicilia (15). Five of the surveyed taxa, for the first time, have been considered invasive aliens to Italian territory. The unfrequent amount of original results provided by this work, over the simple importance of data itself, proves how floristic investigation, still today, represents one of the most effective tools in broadening the current knowledge about alien taxa and their dynamics.

Seed germination and vegetative and in vitro propagation of Hieracium lucidum subsp. lucidum (Asteraceae), a critically endangered endemic taxon of the Sicilian flora.

Hieracium lucidum subsp. lucidum is a critically endangered endemic taxa of the Sicilian flora. It is a relict of the Tertiary period surviving on the cliffs of Monte Gallo (NW-Sicily). This research focused on finding the best protocols for seed germination and vegetative and in vitro propagation to contribute to ex situ conservation. Seed germination tests were carried out using constant temperatures of 15 °C, 20 °C and 25 °C in continuous darkness and an alternating temperature of 30/15 °C (16 h/8 h, light/dark). The seeds had no dormancy, and a high germination capacity (70-95%) was obtained at all tested thermoperiods. The possibility of vegetative propagation of the taxon was evaluated through the rooting capacity of stem cuttings treated or not treated with indole-3-butyric acid (IBA). All cuttings were treated with IBA rooted within 2 months, while only 50% of the untreated cuttings were rooted within a longer time. An efficient protocol for rapid in vitro propagation from leaf portions was developed. The response of explants was tested on hormone-free Murashige and Skoog (MS) basal medium and MS enriched with different types of cytokinins: 6-Benzylaminopurine (BAP) and meta-Topolin (mT) in combination with naphthaleneacetic acid (NAA) and 2,4-Dichlorophenoxyacetic acid (2,4-D) at the same concentration. The combination of mT (2 mg L-1) and 2,4-D (1 mg L-1) in the medium was the most effective and showed the highest percentage of callus induction and the mean number of regenerated shoots. The maximum rate of root regeneration and the maximum number and length of roots were obtained on hormone-free MS and MS enriched with IBA at concentrations of 1 mg L-1. From the results obtained, it can be concluded that H. lucidum subsp. lucidum can be successfully propagated using one of the tested techniques, subject to the availability of the material for reproduction.

Integrative Taxonomy of Armeria Taxa (Plumbaginaceae) Endemic to Sardinia and Corsica.

Sardinia and Corsica are two Mediterranean islands where the genus Armeria is represented by 11 taxa, 10 out of which are endemic. An integrative approach, using molecular phylogeny, karyology, and seed and plant morphometry was used to resolve the complex taxonomy and systematics in this group. We found that several taxa are no longer supported by newly produced data. Accordingly, we describe a new taxonomic hypothesis that only considers five species: Armeria leucocephala and A. soleirolii, endemic to Corsica, and A. morisii, A. sardoa, and A. sulcitana, endemic to Sardinia.

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study.

The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient micropropagation protocol for Adenostyles by evaluating the effect of different plant growth regulators on leaf explants. Adenostyles alpina subsp. macrocephala (Asterace) is a perennial herbaceous plant endemic to Calabria (Southern Italy). The genus Adenostyles includes three species confined to the mountains of the Mediterranean and southern Europe. For callus induction, media supplemented with different concentrations of Benzylaminopurine (BAP) (0.5, 1, 2, and 3 mg L-1), Naphthaleneacetic Acid (NAA) (1 mg L-1), and 2,4-Dichlorophenoxyacetic Acid (2,4-D) (1 mg L-1) were tested. Shoot regeneration and proliferation were obtained in media supplemented with BAP (1, 2, and 3 mg L-1) and NAA (1 mg L-1). Root induction was obtained in media supplemented with IBA (0.25, 0.50, and 1 mg L-1) and NAA (0.25, 0.50, and 1 mg L-1). Statistically significant differences in callus induction and shoot regeneration were observed between the various media tested. The medium containing Murashige and Skoog (MS) supplemented with 3 mg L-1 of BAP and 1 mg L-1 of NAA showed the highest percentage of callus induction and increased shoot regeneration. The regenerated shoots showed more effective root induction in the hormone-free MS medium and in the presence of Indole-3-Butyric Acid (IBA) at concentrations of 0.25, 0.50, and 1 mg L-1. These results can be used as a basis for the preparation of a micropropagation protocol for different taxa of Adenostyles, as well as other species of Asteraceae specialized to the Mediterranean mountain habitat.

Plant invasion risk inside and outside protected areas: Propagule pressure, abiotic and biotic factors definitively matter.

Invasive alien species are among the main global drivers of biodiversity loss posing major challenges to nature conservation and to managers of protected areas. The present study applied a methodological framework that combined invasive Species Distribution Models, based on propagule pressure, abiotic and biotic factors for 14 invasive alien plants of Union concern in Italy, with the local interpretable model-agnostic explanation analysis aiming to map, evaluate and analyse the risk of plant invasions across the country, inside and outside the network of protected areas. Using a hierarchical invasive Species Distribution Model, we explored the combined effect of propagule pressure, abiotic and biotic factors on shaping invasive alien plant occurrence across three biogeographic regions (Alpine, Continental, and Mediterranean) and realms (terrestrial and aquatic) in Italy. We disentangled the role of propagule pressure, abiotic and biotic factors on invasive alien plant distribution and projected invasion risk maps. We compared the risk posed by invasive alien plants inside and outside protected areas. Invasive alien plant distribution varied across biogeographic regions and realms and unevenly threatens protected areas. As an alien's occurrence and risk on a national scale are linked with abiotic factors followed by propagule pressure, their local distribution in protected areas is shaped by propagule pressure and biotic filters. The proposed modelling framework for the assessment of the risk posed by invasive alien plants across spatial scales and under different protection regimes represents an attempt to fill the gap between theory and practice in conservation planning helping to identify scale, site, and species-specific priorities of management, monitoring and control actions. Based on solid theory and on free geographic information, it has great potential for application to wider networks of protected areas in the world and to any invasive alien plant, aiding improved management strategies claimed by the environmental legislation and national and global strategies.

A Nomenclatural and Taxonomic Revision of the Senecio squalidus Group (Asteraceae).

Senecio squalidus (Asteraceae) currently includes nine subspecies distributed in North and Central Europe and in the Mediterranean basin. Within this taxonomic aggregate, many species have been described, but research on their nomenclatural types is incomplete. A complete nomenclatural survey of 19 names belonging to this taxonomically critical group was carried out. Fourteen lectotypes are here designated. The nomenclatural analysis, complemented by field investigations in the type localities of the taxa described in the Central Mediterranean, allowed us to accept 10 species. Accordingly, we proposed here a new name and a new missing combination at a specific level: S. aknoulensis and S. calabrus.

Integrative Taxonomy of Armeria arenaria (Plumbaginaceae), with a Special Focus on the Putative Subspecies Endemic to the Apennines.

Three subspecies of Armeria arenaria are reported from Italy, two of which are considered endemic to the Apennines. The taxonomic value of these two taxa (A. arenaria subsp. marginata and A. arenaria subsp. apennina) is unclear. Moreover, the relationships between A. arenaria subsp. praecox and Northern Italian populations-currently ascribed to A. arenaria subsp. arenaria-have never been addressed. Accordingly, we used an integrated taxonomic approach, including morphometry, seed morpho-colorimetry, karyology, molecular systematics (psbA-trnH, trnQ-rps16, trnF-trnL, trnL-rpl32, and ITS region), and comparative niche analysis. According to our results, French-Northern Italian populations are clearly distinct from Apennine populations. In the first group, there is evidence which allows the recognition of A. arenaria s.str. (not occurring in Italy) and A. arenaria subsp. praecox. In the second group, the two putative taxa endemic to the Northern Apennines cannot be separated, so a single subspecies is here recognized: A. arenaria subsp. marginata.

A new species within the Centaureabusambarensis complex (Asteraceae, Cardueae) from Sicily.

Background: The Centaureabusambarensis group is made up by eight species endemic to Sicily. We statistically evaluated a population found on the Nebrodi Mountain (NE Sicily) to verify if the observed morphological differences with the already known taxa justified the description of a new one. It resulted in being sufficiently distinct to deserve recognition at the species level. New information: Centaureavaldemonensis, a new species endemic to Sicily is described and illustrated here. It is confined to the Nebrodi Mountains (NE Sicily). The distinction of this taxon from the others belonging to the C.busambarensis complex has been supported with the aid of statistical analyses on morphological characters. The differences with the related taxa are discussed.

Comparison of different methods to assess the distribution of alien plants along the road network and use of Google Street View panoramas interpretation in Sicily (Italy) as a case study.

The survey by foot in the field is compared to the survey from a car, the photo-interpretation of Google Street View (GSV) panoramas continuously and at intervals of 1.5 km and the photo-interpretation of Google Earth aerial images on a 10 km stretch of road in Sicily. The survey by foot was used as reference for the other methods. The interpretation of continuous GSV panoramas gave similar results as the assessment by car in terms of the number of species identified and their location, but with lower cost. The interpretation online of aerial photos allowed the identification of a limited number of taxa, but gave a good localisation for them. Interpretation of GSV panoramas, each of 1.5 km, allowed the recognition of twice as many taxa as the interpretation of aerial photos and taking half the time, but did not allow a complete localisation. None of these methods alone seems sufficient to carry out a complete survey. A mixture of different techniques, which may vary according to the available resources and the goal to be achieved, seems to be the best compromise. To further test the capabilities of the survey using the interpretation of GSV panoramas every 1.5 km along the roads, we proceeded to study the alien plants along 3500 km of the road network on the island of Sicily. This survey identified only 10% of the known species for the region, but allowed us to trace the distribution of invasive species whose distribution is currently poorly recorded.

WOODIV, a database of occurrences, functional traits, and phylogenetic data for all Euro-Mediterranean trees.

Trees play a key role in the structure and function of many ecosystems worldwide. In the Mediterranean Basin, forests cover approximately 22% of the total land area hosting a large number of endemics (46 species). Despite its particularities and vulnerability, the biodiversity of Mediterranean trees is not well known at the taxonomic, spatial, functional, and genetic levels required for conservation applications. The WOODIV database fills this gap by providing reliable occurrences, four functional traits (plant height, seed mass, wood density, and specific leaf area), and sequences from three DNA-regions (rbcL, matK, and trnH-psbA), together with modelled occurrences and a phylogeny for all 210 Euro-Mediterranean tree species. We compiled, homogenized, and verified occurrence data from sparse datasets and collated them on an INSPIRE-compliant 10 × 10 km grid. We also gathered functional trait and genetic data, filling existing gaps where possible. The WOODIV database can benefit macroecological studies in the fields of conservation, biogeography, and community ecology.

Nomenclatural Synopsis of Cirsium Sect. Eriolepis (Asteraceae) in Italy.

The names of the Italian taxa in Cirsium sect. Eriolepis are discussed. The accepted names are: Cirsium echinatum, C. eriophorum subsp. eriophorum, C. eriophorum subsp. spathulatum, C. ferox, C. italicum, C. lacaitae, C. lobelii, C. morisianum, C. scabrum, C. tenoreanum, C. vallis-demonii subsp. vallis-demonii, C. vallis-demonii subsp. calabrum comb. nov., and C. vulgare (= C. crinitum, C. sylvaticum). Four accepted names are typified by specimens preserved at FI (one lectotype), G (one lectotype and one neotype), P (one lectotype), and by illustrations (two lectotypes). Several other heterotypic synonyms of taxa described from Italy are discussed and six of them are typified. A new combination and status are proposed: C. vallis-demonii subsp. calabrum, based on C. eriophorum var. vallis-demonii f. calabrum.

Typification of 14 names in the Dianthusvirgineus group (Caryophyllaceae).

The nomenclature of 14 taxa from Central and Southern Europe within the Dianthusvirgineus group is discussed. Dianthusaggericola Jord., D.collivagus Jord., D.consimilis Jord., D.orophilus Jord., D.saxicola Jord., D.juratensis Jord. are here lectotypified by specimens from the Jordan herbarium in LY, while D.godronianus Jord. by a specimen in P. Dianthussubacaulis Vill. is neotypified by a specimen collected on Mont Ventoux (S. France) and housed in MPU. For D.sylvestris Wulfen, a lectotype is here designated and its previous neotypification is discussed. Dianthuscaryophyllusvar.tenuifolius Moris, D.caryophyllusf.minor Moris and D.sylvestrisvar.garganicus Ten. are lectotypified by specimens housed in herbarium Moris (TO) and herbarium Tenore (K). Dianthusvirgineusvar.tergestinus Rchb. is lectotypified by a drawing from the Icones florae Germanicae & Helveticae, while D.contractusvar.evolutus Lojac. is neotypified by a specimen in P. For each taxon the currently accepted name is provided including new synonymies. The type indication is followed by nomenclatural and taxonomic notes, in which the original material found is commented and the reasons for the identification of the types are discussed.

Typification and taxonomic remarks on five species names in Cytisus (Fabaceae).

This paper deals with the typification and taxonomy of five Mediterranean Cytisus species. Cytisus affinis, C. candidus, and C. spinescens nom. illeg., non Sieber ex Spreng. were described from Sicily by Karel Borivoj Presl, Cytisus spinescens was described from Apulia (southern Italy) by Curt Polycarp Joachim Sprengel, and C. villosus was described from southern France by Pierre André Pourret (1788). Lectotypes are here designated for Presl and Sprengel's names. A neotype is designated for C. villosus. The taxonomic revision of these five names confirmed that C. villosus Pourr. (= Cytisus affinis C.Presl) is the name to be used for the species occurring in the large part of the Mediterranean countries. Cytisus spinescens Sieber ex Spreng. (≡ C. candidus C.Presl = C. spinescens C.Presl, nom. illeg.) is the correct name for the amphi-adriatic species occurring in peninsular Italy, and along the NE coast of the Adriatic Sea. This species does not occur in Sicily and reference to this latter region in the protologues of both C. spinescens C.Presl and C. candidus C.Presl is a misinterpretation due, possibly, to exchange of labels.

Synthetic cartography for mapping biodiversity in the Mediterranean region: Sicily as a case study.

This paper proposes a new hierarchical land classification system for the mapping of species distribution at national or regional scales. Our integrative framework incorporates two hierarchical levels inferred from historical, climatic, geomorphological and geological attributes. The feasibility of this proposal is based on the use of historical collections and literature data, as well as on its ability to combine old low-precision data with more recent records of higher resolution. The system is set up for vascular plants, but it can also be used for other taxonomic groups. Furthermore, it has the potential for application to the whole Mediterranean region because it is based on information that is generally available in all Mediterranean countries. This model is tested with the distribution of loci classici of the Italian endemic plants occurring in Sicily.