Relationship between macrophage autophagy and atherosclerosis:research advances / 国际药学研究杂志

Macrophage plays important roles in the formation of atherosclerotic plaque,and the role of macrophage autophagy in the process of atherosclerosis has drawn increasing attention from researchers. The lysosomal system degrades damages,degenerat- ed,senescent organelles and proteins in the cytoplasm. Basic level of autophagy can protect the cells from environmental stimuli, while excessive autophagy can lead to cell death and do harm to the stability of the plaque. The regulation of macrophage autophagy can affect the development of atherosclerosis. In this paper,the regulation of macrophage signal pathway in the development of athero- sclerosis,the factors that induce autophagy and other factors are reviewed systematically in order to provide a new target for the treat- ment of atherosclerosis.

[Detection and analysis of ABO Hemolytic disease in newborn].

This study was purposed to investigate the incidence and the model of ABO hemolytic disease in newborn (ABO-HDN) and the results of the three hemolysis test, so as to provide the evidences for clinical diagnosis and therapy. A total of 227 cases of maternal-fetal ABO incompatibility from January 2013 to October 2013 in the First Affiliated Hospital of Xiamen University were enrolled in the study. The ABO blood group of newborn and mother was detemined and three hemolysis tests (direct antiglobulin test, free antibody test, RBC antibody release test) were performed. The results indicated that in 227 cases of ABO incompatible pregnancies,186 cases were ABO-HDN (81.94%). There was no significant difference in the incidence between O-A and O-B incompatible pregnancies (P > 0.05). The positive ratio of direct antiglobulin test, free antibody test and RBC antibody release test were 59.14% (110/186), 84.78% (156/186) and 94.62% (176/186) respectively. It is concluded that the incidence of ABO-HDN is high. The main models of ABO-HDN were O-A and O-B. There was no significant difference in the incidence between O-A and O-B incompatible pregnancies. Three hemolysis tests are high sensitivity and are helpful in early diagnosis and early treatment of HDN.

[Rapid propagation system for tissure culture of Smilax glabra].

OBJECTIVE: Establishing the rapid propagation for plant tissue culture of Smilax glabra in order to rationally protect and utilize the resource. METHODS: The seed, shoot, leaf and rhizome of Similax glabra were cultured as explants. Different media and plant growth regulators at different concerntration were used. RESULTS: The best medium for bud induction was MS + 6-BA 2.0 mg/L + NAA 0.05 mg/L; The optimal medium for proliferation culture was MS +6-BA 2.0 mg/L + NAA 0.5 mg/L; The optimal medium for rooting was H + IBA 0.5 mg/L + NAA 0.5 mg/L; The optimal stroma for seedlings was 50% peat + 50% river sand. CONCLUSION: Different explants except leaf can be induced for tissue culture and rhizome has the optimal bud induction rate. An effective approach for rapid propagation has been provided,which can promote the artificial cultivation of Smilax glabra.

[Evaluation on germplasm resources of main production area of Artemisia annua in China].

OBJECTIVE: To evaluate and select excellent germplasm resources of Artemisia annua L., providing basic data for data base and breeding of A. annua. METHOD: Seventy-two germplasms of A. annua, which were collected from main production areas, were planted in germplasm resources garden in Jingxi and Nanning under the same conditions. The samples were gathered in the squaring stage. Artemisinin was extracted by supersonic wave and determined by ultraviolet spectrophotometry. RESULT: The leaf's yield and the content of artemisinin in the samples of different germplasms were varied significantly. Effect of environment on artemisinin content was more than that of hereditary factors. The content of artemisinin was different when the same material of A. annua grew in different place. The content of artemisinin in next year's plant was decreasing. CONCLUSIONS: Seven germplasms from south of China have been selected, their content of artemisinin was above 0.9% and calculated yield was more than 2250 kg x hm(-2). The content of artemisinin has been affected by hereditary factors and variation of the growth environment.