RESUMO
We have constructed a colorimetric protein assay platform based on a nanoreactor. The novel strategy presents high sensitivity and excellent performance for the detection of thrombin. The linear range is 5.58 × 10(-10) to 6.50 × 10(-9) M and the detection limit down to 0.19 nM is achieved.
Assuntos
Colorimetria , Nanotecnologia , Trombina/análise , Benzidinas/química , Biocatálise , Técnicas Biossensoriais , Peróxido de Hidrogênio , Nanopartículas de Magnetita/química , OxirreduçãoRESUMO
Both coordination and hydrogen bonds contribute to networking in the supramolecular title compound, [Co(C(6)H(6)NO(3)S)(C(12)H(8)N(2))(H(2)O)(3)]Cl, which contains a discrete [Co(C(6)H(6)NO(3)S)(C(12)H(8)N(2))(H(2)O)(3)](+) complex cation, formed by one 4-aminobenzenesulfonate ligand, one 1,10-phenanthroline ligand and three coordinated water molecules, together with one uncoordinated chloride anion. These discrete cations and chloride anions are connected by hydrogen-bonding interactions into a two-dimensional supramolecular motif. Further hydrogen-bonding interactions consolidate the structural architecture and extend the two-dimensional supramolecular structure into a three-dimensional network.
RESUMO
OBJECTIVE: To use a new kind of fixing material, i.e. Sol-Gel organic-inorganic hybridized material to immobilize bacterium to detect Biochemical oxygen demand quickly. METHODS: The biosensor was fabricated using a thin film in which Hansenula anomala was immobilized by sol-gel and an oxygen electrode. The optimum measurement for biochemical oxygen demand was at pH 7.0; 28 degrees C; response time 3 - 12 min. Pure organic compound, sewage and rate of recovery were detected with the biosensor. RESULTS: It shows that the BOD biosensor can be used to detect many organic compounds such as amino acid, glucide. It is suitable to monitor sewage and industrial waste water which has low level alcohols and phenols. The microbial membrane can work 3 months and remain its 70% activity. It is measured that the rate of recovery of BOD is between 90% to 105% in sewage. CONCLUSION: The study confirmed the effectiveness and usefulness of BOD sensor, which is quick, convenient, low cost and reliable with little interference.