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1.
Plants (Basel) ; 11(9)2022 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-35567145

RESUMO

Rootstock grafting is an important method to improve the yield and quality of seedlings. Pumpkin is the rootstock of watermelon, melon, and cucumber, and the root phenotype of rootstock is an important reference for breeding. At present, the root phenotype is mainly measured by scanners, with which it is difficult to achieve non-destructive and in situ measurements. In this work, we propose a method for non-destructive measurement of the root phenotype on the surface layer of the root ball of pumpkin rootstock plug seedlings and an accurate estimation of the surface area, length, and volume of total root using an AZURE KINECT sensor. Firstly, the KINECT is used to capture four-view color and depth images of the root surface, and then multi-view images are spliced to obtain a complete image of the root surface. After preprocessing of the images, we extract the roots from the root ball. For root phenotype measurements, the surface areas of the surface roots and root ball are calculated, followed by calculating root encapsulation. Next, the non-overlapping roots in the surface root image are extracted, and the ratio of the surface area to the skeleton length is used as the average diameter of total root. Based on the high correlation between the surface area of surface root and the surface area of total root, a linear fitting model is established to estimate the surface area, length, and volume of total root. The experiment ultimately showed that the measurement error for the average diameter of total root is less than 30 µm, and consistency with the scanner is higher than 93.3%. The accuracy of the surface area of total root estimation was found to be more than 88.1%, and the accuracy of the root length of total root estimation was observed to be greater than 87.2%. The method proposed in this paper offers similar accuracy to a scanner, which meets the needs of non-destructive root phenotype research. This method is expected to replace root scanners for high-throughput phenotypic measurements and provides a new avenue for root phenotype measurements of pumpkin rootstocks. This technology will provide key basic data for evaluating the root growth of pumpkin rootstocks.

2.
Materials (Basel) ; 14(20)2021 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-34683524

RESUMO

The abrasion failure is the key factor for prolonging the service life and energy saving of furrow openers. The hardness enhancement was reported to be an effective strategy to increase the wear resistance against the soil abrasion. D517 coatings were deposited on Q235 steel by electric spark to improve the wear-resistant property with an affordable cost for farmers. The wear behavior of the coatings was characterized in a pin on disk friction equipment and a homemade soil abrasion simulation system. The soil adhesion, which is highly related to energy consumption, was also evaluated. Results showed that D517 coatings revealed dendrite structure with some randomly distributed carbides. The electric current exerted a great influence on the microstructure, hardness, friction coefficient, and soil wear rate. The wear rate of samples deposited with 80 A and 90 A reduced to 79% and 84%, respectively, as compared with the normalized heat-treated 65 Mn steel after 6 h in soil. This work provides a promising solution to increase the wear resistance of furrow openers. It needs to be noted that the coating would increase the soil adhesion of the opener, which needs to be further explored to decrease the energy consumption.

3.
Sensors (Basel) ; 21(14)2021 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-34300368

RESUMO

The three-dimensional reconstruction method using RGB-D camera has a good balance in hardware cost and point cloud quality. However, due to the limitation of inherent structure and imaging principle, the acquired point cloud has problems such as a lot of noise and difficult registration. This paper proposes a 3D reconstruction method using Azure Kinect to solve these inherent problems. Shoot color images, depth images and near-infrared images of the target from six perspectives by Azure Kinect sensor with black background. Multiply the binarization result of the 8-bit infrared image with the RGB-D image alignment result provided by Microsoft corporation, which can remove ghosting and most of the background noise. A neighborhood extreme filtering method is proposed to filter out the abrupt points in the depth image, by which the floating noise point and most of the outlier noise will be removed before generating the point cloud, and then using the pass-through filter eliminate rest of the outlier noise. An improved method based on the classic iterative closest point (ICP) algorithm is presented to merge multiple-views point clouds. By continuously reducing both the size of the down-sampling grid and the distance threshold between the corresponding points, the point clouds of each view are continuously registered three times, until get the integral color point cloud. Many experiments on rapeseed plants show that the success rate of cloud registration is 92.5% and the point cloud accuracy obtained by this method is 0.789 mm, the time consuming of a integral scanning is 302 s, and with a good color restoration. Compared with a laser scanner, the proposed method has considerable reconstruction accuracy and a significantly ahead of the reconstruction speed, but the hardware cost is much lower when building a automatic scanning system. This research shows a low-cost, high-precision 3D reconstruction technology, which has the potential to be widely used for non-destructive measurement of rapeseed and other crops phenotype.


Assuntos
Brassica napus , Imageamento Tridimensional , Algoritmos , Produtos Agrícolas
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