Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros











Base de dados
Tipo de estudo
Intervalo de ano de publicação
1.
Genet Mol Res ; 15(2)2016 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-27420979

RESUMO

The bovine TRIM28 gene was amplified from ovary tissue by using RT-PCR. The TRIM28 gene was inserted into the eukaryotic expression vector pIRES2-EGFP and transfected into bovine fetal fibroblasts by using Lipofectamine 3000. TRIM28 mRNA and protein were detected by fluorescence microscope and western blotting. The results showed that the full length of TRIM28 was cloned and pIRES2-EGFP-TRIM28 was constructed successfully. EGFP expression was observed, and the pIRES2-EGFP-TRIM28 transfected group expressed more TRIM28 protein than that by the pIRES2-EGFP group. The TIMR28 gene has been successfully transferred into bovine fetal fibroblasts.


Assuntos
Proteínas Repressoras/genética , Animais , Bovinos , Células Cultivadas , Clonagem Molecular , Feminino , Fibroblastos/metabolismo , Vetores Genéticos/genética , Ovário/metabolismo , Proteínas Repressoras/metabolismo
2.
Genet Mol Res ; 11(3): 1841-50, 2012 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-22869540

RESUMO

We examined a possible relationship -420C>G SNP of the resistin gene with plasma resistin and C-reactive protein concentrations in intracerebral hemorrhage. Three hundred and forty-four Chinese Han patients with intracerebral hemorrhage and 344 age- and gender-matched healthy controls were included in our study. Plasma resistin and C-reactive concentrations were measured and SNP -420C>G was genotyped. The genotype frequencies in controls and patients were not significantly different (P = 0.672). Plasma resistin and C-reactive protein levels were significantly different between the SNP -420C>G genotypes, even after adjustment for age, gender and body mass index. The common homozygote (C-C) had the lowest resistin and C-reactive protein plasma concentrations; the plasma resistin and C-reactive protein concentrations in the heterozygote (C-G) and the rare allele homozygote (G-G) did not differ significantly. Plasma resistin levels were significantly associated with plasma C-reactive protein level. We conclude that SNP -420C>G of the resistin gene could be involved in the inflammatory component of intracerebral hemorrhage through enhanced production of resistin.


Assuntos
Povo Asiático/genética , Hemorragia dos Gânglios da Base/genética , Proteína C-Reativa/metabolismo , Etnicidade/genética , Polimorfismo de Nucleotídeo Único/genética , Resistina/sangue , Resistina/genética , Idoso , Idoso de 80 Anos ou mais , Hemorragia dos Gânglios da Base/sangue , China , Feminino , Frequência do Gene/genética , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Masculino , Pessoa de Meia-Idade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA