Correlations between root phosphorus acquisition and foliar phosphorus allocation reveal how grazing promotes plant phosphorus utilization.

Overgrazing and phosphorus (P) deficiency are two major factors limiting the sustainable development of grassland ecosystems. Exploring plant P utilization and acquisition strategies under grazing can provide a solid basis for determining a reasonable grazing intensity. Both foliar P allocation and root P acquisition are crucial mechanisms for plants to adapt to environmental P availability; however, their changing characteristics and correlation under grazing remain unknown. Here, we investigated foliar P fractions, root P-acquisition traits and gene expression, as well as rhizosphere and bulk soil properties of two dominant plant species, Leymus chinensis (a rhizomatous grass) and Stipa grandis (a bunchgrass), in a field grazing intensity gradient site in Inner Mongolia. Grazing induced different degrees of compensatory growth in the two dominant plant species, increased rhizosphere P availability, and alleviated plant P limitation. Under grazing, the foliar metabolite P of L. chinensis increased, whereas the nucleic acid P of S. grandis increased. Increased P fractions in L. chinensis were positively correlated with increased root exudates and rapid inorganic P absorption. For S. grandis, increased foliar P fractions were positively correlated with more fine roots, more root exudates, and up-regulated expression of genes involved in defense and P metabolism. Overall, efficient root P mobilization and uptake traits, as well as increases in leaf metabolic activity-related P fractions, supported plant compensatory growth under grazing, a process that differed between tiller types. The highest plant productivity and leaf metabolic activity-related P concentrations under medium grazing intensity clarify the underlying basis for sustainable livestock production.

Soil phosphorus availability alters the correlations between root phosphorus-uptake rates and net photosynthesis of dominant C3 and C4 species in a typical temperate grassland of Northern China.

Phosphorus (P) fertilization can alleviate a soil P deficiency in grassland ecosystems. Understanding plant functional traits that enhance P uptake can improve grassland management. We measured impacts of P addition on soil chemical and microbial properties, net photosynthetic rate (Pn ) and nonstructural carbohydrate concentrations ([NSC]), and root P-uptake rate (PUR), morphology, anatomy, and exudation of two dominant grass species: Leymus chinensis (C3 ) and Cleistogenes squarrosa (C4 ). For L. chinensis, PUR and Pn showed a nonlinear correlation. Growing more adventitious roots compensated for the decrease in P transport per unit root length, so that it maintained a high PUR. For C. squarrosa, PUR and Pn presented a linear correlation. Increased Pn was associated with modifications in root morphology, which further enhanced its PUR and a greater surplus of photosynthate and significantly stimulated root exudation (proxied by leaf [Mn]), which had a greater impact on rhizosheath micro-environment and microbial PLFAs. Our results present correlations between the PUR and the Pn of L. chinensis and C. squarrosa and reveal that NSC appeared to drive the modifications of root morphology and exudation; they provide more objective basis for more efficient P-input in grasslands to address the urgent problem of P deficiency.

Changes in plant phosphorus demand and supply relationships in response to different grazing intensities affect the soil organic carbon stock of a temperate steppe.

Ongoing climate change and long-term overgrazing are the main causes of grassland degradation worldwide. Phosphorus (P) is typically a limiting nutrient in degraded grassland soils, and its dynamics may play a crucial role in the responses of carbon (C) feedback to grazing. Yet how multiple P processes respond to a multi-level of grazing and its impact on soil organic carbon (SOC), which is critical for sustainable grassland development in the face of climate change, remains inadequately understood. Here, we investigated P dynamics at the ecosystem level in a 7-year-long multi-level grazing field experiment and analyzed their relation to SOC stock. The results showed that, due to the greater P demand for compensatory plant growth, grazing by sheep increased the aboveground plants' P supply (by 70 % at most) while decreasing their relative P limitation. The increase in P in aboveground tissue was associated with changes in plant root-shoot P allocation and P resorption, and the mobilization of moderately labile organic P in soil. Affected by the altered P supply under grazing, corresponding changes to root C stock and soil total P were two major factors impacting SOC. Compensatory growth-induced P demand and P supply processes responded differently to grazing intensity, resulting in differential effects on SOC. Unlike the light and heavy grazing levels, which reduced the SOC stock, moderate grazing was capable of maintaining maximal vegetation biomass, total plant biomass P, and SOC stock, mainly by promoting biologically- and geochemically-driven plant-soil P turnover. Our findings have important implications for addressing future soil C losses and mitigating higher atmospheric CO2 threats, as well as maintaining high productivity in temperate grasslands.

Carbon isotopic measurements from coastal zone protected forests in northern China: Soil carbon decomposition assessment and its influencing factors.

Panting protected forests to increase soil carbon sequestration is an effective means of reducing carbon emissions. Soil organic carbon (SOC) decomposition is one of the main indicators of soil carbon sequestration. However, SOC decomposition and its influencing factors in protected forests have not been fully characterized, especially in coastal zones. In this paper, coastal zone protected forest stands composed of Quercus acutissima Carruth (QAC), Pinus thunbergii Parl (PTP) and mixed PTP and QAC (MF) were selected as the research objects. The trends of the SOC decomposition rate were characterized by the beta (ß) value, and the influencing factors were further explored with structural equation models. The results were as follows: The SOC content decreased from leaf to litter and then to the soil profile at all sites, while the δ13C value increased. The ß value ranged from -3.12 to -5.76, with an average of -3.81. The ß value was positively correlated with the diversity and richness of soil bacteria, supporting the hypothesis that the increase in δ13C with depth was mainly caused by isotope fractionation in the process of microbial SOC decomposition. The structural equation model showed that nitrogen and the availability of nitrogen have a strong ability to explain the value of ß, which indicates that nitrogen-based edaphic variables play an important role in affecting SOC decomposition. The SOC decomposition rate in PTP was higher than that in QAC and MF. The results of this study indicate that the prediction of SOC decomposition based on the ß value is suitable for coastal zone protected forests. The incorporation of edaphic variables into global carbon cycle models may enhance the predictions of SOC dynamics in coastal zone protected forests.