[Preparation and purification of polyclonal antibody against Toxoplasma gondii microneme protein 16 and its application in subcellular localization].

OBJECTIVE: To prepare and purify the rabbit anti-TgMIC16 polyclonal antibody, so as to apply it in subcellular localization. METHODS: New Zealand white rabbits were immunized with purified recombinant TgMIC16 mixing with the same volume of Freund's adjuvant for three times, respectively. The rabbit serum was collected on the 14th day after the last immunization. The polyclonal antibody in rabbit serum was purified with Protein A affinity purification column. ELISA and Western blotting were used to detect the antibody titer and specificity of polyclonal antibody. The polyclonal antibody was used to the localization of TgMIC16 by the immunofluorescence method. RESULTS: Indirect ELISA showed that the antibody titer was 1∶512 000. Western blotting showed that the recombinant TgMIC16 protein was recognized by the specific polyclonal antibody. IFA showed that TgMIC16 was located in the microneme of Toxoplasma gondii. CONCLUSIONS: The rabbit anti-TgMIC16 is prepared and purified, and successfully applied to immunofluorescence localization of TgMIC16 in T. gondii.

[Overwintering surveillance of Culex pipiens pallens in Shandong Province].

OBJECTIVE: To understand the ecological habits of Culex pipiens pallens in Shandong Province in winter. METHODS: From December 2015 to January 2016, the overwintering conditions of Cx. pipiens pallens were investigated in Shandong Province. RESULTS: In Shandong Province, in rural districts, the overwintering places of Cx. pipiens pallens were basements, wells and caves; and in urban areas, they were air raid shelters, holes of city walls, sewers and flower cellars. CONCLUSIONS: In Shandong Province, the overwintering places of Cx. pipiens pallens are mainly basements and holes, which are under high temperature and humidity, and away from light. Its larvae cannot overwinter.

Preparation and purification of polyclonal antibody against Toxoplasma gondii microneme protein 16 and its application in subcellular localization / 中国血吸虫病防治杂志

To prepare and purify the rabbit anti-TgMIC16 polyclonal antibody, so as to apply it in subcellular localization.New Zealand white rabbits were immunized with purified recombinant TgMIC16 mixing with the same volume of Freund’s adjuvant for three times, respectively. The rabbit serum was collected on the 14th day after the last immunization. The polyclonal antibody in rabbit serum was purified with Protein A affinity purification column. ELISA and Western blotting were used to detect the antibody titer and specificity of polyclonal antibody. The polyclonal antibody was used to the localization of TgMIC16 by the immunofluorescence method.Indirect ELISA showed that the antibody titer was 1∶512 000. Western blotting showed that the recombinant TgMIC16 protein was recognized by the specific polyclonal antibody. IFA showed that TgMIC16 was located in the microneme of Toxoplasma gondii.The rabbit anti-TgMIC16 is prepared and purified, and successfully applied to immunofluorescence localization of TgMIC16 in T. gondii.

Overwintering surveillance of Culex pipiens pallens in Shandong Province / 中国血吸虫病防治杂志

Objective To understand the ecological habits of Culex pipiens pallens in Shandong Province in winter. Meth-ods From December 2015 to January 2016,the overwintering conditions of Cx.pipiens pallens were investigated in Shandong Province. Results In Shandong Province,in rural districts,the overwintering places of Cx. pipiens pallens were basements, wells and caves;and in urban areas,they were air raid shelters,holes of city walls,sewers and flower cellars.Conclusion In Shandong Province,the overwintering places of Cx.pipiens pallens are mainly basements and holes,which are under high tem-perature and humidity,and away from light.Its larvae cannot overwinter.

Change of corrected vision and stereo vision in anisometropic patients after LASIK / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To investigate the change of corrected vision and stereo vision in anisometropic patients after laser in situ keratomileusis (LASIK).METHODS: The clinical data of 84 cases of anisometropic children(84 eyes) were retrospective analyzed.The changes of corrected visual acuity and stereopsis of different gender,age,type of amblyopia in children anisometropic before treatment,after treatment for 3,6mo and 1a were analyzed.The stereoscopic vision correction correlation were analyzed.RESULTS: Corrected Log MAR visual acuity and stereopsis of 84 patients after LASIK surgery treatment for 3mo,and 1a later significantly decreased than before treatment (P0.05).Corrected visual acuity of age 0.05).Improved correctted vision of anisometropic patients after LASIK surgery had no connection with the decrease of stereo vision(P>0.05).CONCLUSION: LASIK surgery can improve visual acuity in patients with anisometropia amblyopia,but the course of treatment of visual acuity and stereopsis affected by age and type of amblyopia.

[Investigation on Toxoplasma gondii infection in reproductive women in Shandong Province].

OBJECTIVE: To investigate the Toxoplasma gondii infection situation and relative factors in reproductive women in Shandong area, so as to provide the evidences for establishing strategies for reducing the birth defects and improving the quality of population. METHODS: A total of 5 386 reproductive women who visited hospitals at county and city levels in Shandong region from January 2013 to December 2015 were treated as the research objects, and their venous blood samples were collected to detect the antibodies (IgG and IgM) against T. Gondii in serum. Meanwhile, the related risk factors of T. gondii infection were surveyed by questionnaires. RESULTS: Among the 5 386 reproductive women, 623 ones were positive for IgG antibody and the positive rate was 11.56%, and 328 ones were positive for IgM antibody and the positive rate was 6.21%. The single factor analysis showed that the pregnant status (χ2 = 13.12, P < 0.01), whether the residences were in downtowns or counties (χ2 = 6.27, P < 0.05), whether having animal contact history (χ2 = 10.46, P < 0.01), and whether eating half-baked foods (χ2 = 21.19, P < 0.01) were related to T. gondii infection. CONCLUSIONS: The T. gondii infection rate of reproductive women in Shandong Province is high; the related risk factors include pregnant status, residence, degree of intimate contact with animal, and whether eating half-baked foods. It suggests that the detection of T. gondii infection and health education in reproductive women should be strengthened.