Determination of Benzoic Acid and Cinnamic Acid in Suhexiang Pills by HPLC with Dual Wavelength De-tection / 中国药师

Objective:To establish an HPLC method with dual wavelength detection for the determination of benzoic acid and cin-namic acid in Suhexiang pills. Methods:A Waters Sun Fire TM C18 column(150 mm × 4. 6 mm,5 μm) was adopted with 0. 1% ace-tic acid-methanol(60∶40) as the mobile phase at a flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 228nm for benzoic acid(0-18min) and 285nm for cinnamic acid (18. 1-35min). The injection volume was 10μl, and the column temperature was room temperature. Results:The linear range of benzoic acid and cinnamic acid was 1. 0-50. 0 μg·ml-1(r=0. 999 7) and 0. 2-10. 0 μg· ml-1, respectively(r=0. 999 9). The average recovery was 96. 88%(RSD=1. 6%, n=9) and 99. 35%(RSD=1. 7%, n=9), re-spectively. Conclusion:The method is accurate, fast and simple in the determination of benzoic acid and cinnamic acid in Suhexiang pills.

Comparison between transsylvian-transinsular approach and transcortical approach for hematoma evacuation in hypertensive basal ganglia hemorrhage / 国际脑血管病杂志

Objective To compare the efficacy of hematoma evacuation between transsylvian-transinsular approach and transcortical approach in hypertensive basal ganglia hemorrhage.Methods The patients with hypertensive basal ganglia hemorrhage who underwent hematoma evacuation via transsylviantransinsular approach and transcortical approach were enrolled retrospectively.Demographics and baseline data,as well as the outcome (the modified Rankin scale 0-3 as good outcome and ≥4 as poor outcome) and mortality at 3 months were compared in both groups.Results A total of 68 patients with hypertensive cerebral hemorrhage (40 cases via transsylvian-transinsular approach and 28 via transcortical approach) were enrolled.There were no significant differences in the demographics and baseline data between the two groups (all P＞ 0.05).The good outcome rates in the transsylvian-transinsular approach and transcortical approach at 3 months after surgery were 52.50％ (21/40) and 21.43％ (6/28),respectively.The former is significantly higher than the latter (x2 =6.642; P=0.01); the mortalities in the transsylvian-transinsular approach and transcortical approach were 2.50％ (1/40) and 21.43％ (6/28),respectively.The former is significantly lower than the latter (Fisher's exact test,P=0.017).Conclusions The clinical efficacy of hypertensive basal ganglia hemorrhage via transsylvian-transinsular approach is better than the transcortical approach.

The influence between managements in emergency room and outcome of severe traumatic brain injury / 中国医师进修杂志

Objective To assess the influence between managements in emergency room(ER) andoutcome of severe traumatic brain injury (TBI),in order to provide inference for treatment.Methods A retrospective analysis was performed in severe TBI patients and recorded next indexes.(1) The managements in ER,including endotracheal intubation and oxygenation,fluid resuscitation,and mannitol intake.(2) The vital signs arriving at ICU,including systolic pressure and blood oxygen saturation.(3) Prognostic indicators including inhospital mortality and days during ICU,the scores of Glasgow outcome scale (GOS) at discharge and 6 months after injury.Results In 140 severe TBI patients,65 patients (46.4％) died during ICU.The mortality of patients with endotracheal intubation [65.0％ (39/60)] was significantly higher than that without endotracheal intubation [32.5％(26/80)](P＜ 0.01).The mortality in whether fluid resuscitation and using mannitol had no significant difference [44.7％ (46/103) vs.51.4％ (19/37),49.2％ (31/63) vs.44.2％ (34/77)] (P ＞0.05).In days during ICU,there was no significant difference among the three treatment measures (P＞ 0.05).In GOS grade at discharge and 6 months after injury,the proportion of 4 and 5 grade were 8.3％ (5/60) and 25.0％ (15/60) in patients with endotracheal intubation,while 27.5％ (22/80) and 52.5％ (42/80) in patients without endotraeheal intubation (P ＜ 0.01).In fluid resuscitation and using mannitol patients,there were no significant difference(P ＞ 0.05).Conclusion Treating severe TBI patients in ER,endotracheal intubation should be carefully chosen,fluid resuscitation and mannitol may not be given.

Survivin siRNA inhibits gastric cancer in nude mice.

The objective of the study was to evaluate the expression of survivin, cell proliferation, and apoptosis in survivin-specific siRNA-transfected human gastric cancer cell line MGC-803. For this purpose, the target gene fragments were cloned into pSilencer3.1-Hl neo vector. Recombinant eukaryotic expression vector, pSilencer3.1-SVV was successfully constructed and then the recombinant vector was transfected into gastric cancer MGC-803 cells. The mRNA expression of survivin was determined by reverse-transcriptase polymerase chain reaction (RT-PCR). Survivin protein expression was detected by Western blot. Cell cycle distribution and apoptosis were determined by flow cytometry. Our data regarding RT-PCR and Western blot showed that pSilencer3.1-SVV vector could knockdown the expression of survivin mRNA and protein. In contrast with the control group, the apoptotic index of MGC-803 cells increased remarkably. Survivin-specific siRNA caused cells accumulation in the G2/M phase and the number of cells in the G0/G1 phase decreased after transfection. It was, therefore, concluded that the siRNA targeting survivin gene could inhibit the proliferation of gastric cancer cells and induce apoptosis. The use of survivin siRNA may provide a novel approach for gene therapy of gastric cancer.

The study of relationship between lung function and IFN-γ, IL-4 levels in peripheral blood in patients with pulmonary tuberculosis / 中国基层医药

Objective To explore the correlation of lung function and IFN-γ and IL-4 levels in peripheral blood in pulmonary tuberculosis patients. Methods The IFN-γ and IL-4 levels in peripheral blood of 75 pulmonary tuberculosis patients(severe 25 cases,moderate 25 casea,and mild 25 cases) and 30 healthy volunteers were measured by ELISA. Meanwhile FEV1 ,FEV1% and MMEF% of lung function in 75 pulmonary tuberculosis patients were measured.Results The levels of IFN-γ[(0. 204 ±0. 018) μg/L] and IL-4[(0. 523 ±0. 035) μg/L] in peripheral blood were significantly different both between tuberculosis group and control group and among tuberculosis patients ( severe,moderate,and mild) (t =7. 685,6. 374 ,all P ＜0. 05) ;FEV1 ,FEV1% ,and MMEF% of severe and moderate tuberculosis patients were significantly lower than those of mild tuberculosis patients and normal reference value; In tuberculosis patients, FEV1% and MMEF% were negatively related with IL-4 level in peripheral blood( r = -0. 46, -0. 43, all P ＜ 0. 05 ), also significantly positively related with the IFN-γ level in peripheral blood ( r = 0. 47,0. 45, all P ＜0. 05). Conclusion Pulmonary tuberculosis morbility may be due to cellular levels of patients. The IFN-γ and IL-4 levels in peripheral blood could affect the lung function of pulmonary tuberculosis patients.