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8.
Clin Exp Dermatol ; 41(1): 38-42, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26053680

RESUMO

Acne is a common and complex skin disease, with a very complex pathogenesis. Although in women the relationship between acne and insulin resistance is well known, in particular in women with PCOS, in males this relationship has been poorly investigated. In total, 20 subjects with an altered metabolic profile were considered for this study and randomized as follows: 10 patients were treated with metformin plus a hypocaloric diet for 6 months (group A), while 10 patients did not receive any treatment with metformin and were only followed up (group B). All patients of group A, after 6 months of metformin therapy, had a statistically significant improvement compared with patients in group B. Our study reveals the importance of diet and insulin resistance in acne pathogenesis, and underlines the possible use of metformin and diet as possible adjuvant therapy for male patients with acne.


Assuntos
Acne Vulgar/terapia , Dieta Redutora , Hipoglicemiantes/uso terapêutico , Metformina/uso terapêutico , Acne Vulgar/patologia , Adolescente , Adulto , Terapia Combinada , Glucose/metabolismo , Índice Glicêmico , Humanos , Insulina/metabolismo , Resistência à Insulina/fisiologia , Masculino , Adulto Jovem
9.
Hum Mutat ; 29(6): E27-36, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18429042

RESUMO

We characterized 29 unrelated patients presenting with the severe form of Pompe disease (Glycogen Storage Disease Type II, acid maltase deficiency) and identified 26 pathogenic mutations divided over 28 different genotypes. Among the eight new mutations, five were exonic point mutations (c.572A>G, c.1124G>T, c.1202A>G, c.1564C>G and c.1796C>A) leading to codon changes (p.Y191C, p.R375L, p.Q401R, p.P522A and p.S599Y); two were intronic point mutations (c.-32-3C>A and c.1636+5G>C) affecting mRNA processing; one was a single base deletion (c.742delC) generating a truncated protein (p.L248PfsX20). A comprehensive evaluation, based on different methodological approaches, confirmed the detrimental effect of the eight mutations on the protein and its function. Structural alterations potentially induced by the five missense mutations were also predicted through visual inspection of the atomic model of the GAA protein, in terms of both function and spatial orientation of specific residues as well as disturbance generated by amino acid substitutions. Although the remarkable heterogeneity of the mutational spectrum in Pompe disease was already known, our data demonstrate and confirm the power of molecular and functional analysis in predicting the natural course of Pompe disease.


Assuntos
Doença de Depósito de Glicogênio Tipo II/genética , Mutação , alfa-Glucosidases/genética , Animais , Células COS , Pré-Escolar , Chlorocebus aethiops , Análise Mutacional de DNA , Éxons , Deleção de Genes , Humanos , Lactente , Íntrons , Modelos Moleculares , Mutação de Sentido Incorreto , Mutação Puntual , alfa-Glucosidases/química
10.
Hum Mutat ; 27(10): 999-1006, 2006 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-16917947

RESUMO

Glycogen storage disease type II (GSDII) is a recessively inherited disorder due to the deficiency of acid alpha-glucosidase (GAA) that results in impaired glycogen degradation and its accumulation in the lysosomes. We report here the complete molecular analysis of the GAA gene performed on 40 Italian patients with late onset GSDII. Twelve novel alleles have been identified: missense mutations were functionally characterized by enzyme activity and protein processing in a human GAA-deficient cell line while splicing mutations were studied by RT-PCR and in silico analysis. A complex allele was also identified carrying three different alterations in cis. The c.-32-13T > G was the most frequent mutation, present as compound heterozygote in 85% of the patients (allele frequency 42.3%), as described in other late onset GSDII Caucasian populations. Interestingly, the c.-32-13T > G was associated with the c.2237G > A (p.W746X) in nine of the 40 patients. Genotype-phenotype correlations are discussed with particular emphasis on the subgroup carrying the c.-32-13T > G/c.2237G > A genotype.


Assuntos
Doença de Depósito de Glicogênio Tipo II/genética , Mutação/genética , alfa-Glucosidases/genética , Adolescente , Adulto , Idade de Início , Idoso , Alelos , Western Blotting/métodos , Criança , Pré-Escolar , Análise Mutacional de DNA/métodos , Éxons/genética , Feminino , Fibroblastos/metabolismo , Frequência do Gene , Genótipo , Doença de Depósito de Glicogênio Tipo II/epidemiologia , Doença de Depósito de Glicogênio Tipo II/etnologia , Humanos , Itália , Masculino , Pessoa de Meia-Idade , Fenótipo , alfa-Glucosidases/metabolismo
11.
Ann Sclavo ; 20(5): 707-12, 1978.
Artigo em Italiano | MEDLINE | ID: mdl-111632

RESUMO

The selection of antibiotic resistant Pseudomonas aeruginosa strains by an indiscriminate administration of antibiotics has been observed. The consequences induced by Ps. aeruginosa on the tissue culture cell lines (both primary and continuos) are harmful, velding a sharp pH drop from a slight initial increase, followed by the destruction of the cell layer. Sometimes the cells succeed in growing up for some passages since antibiotics are usually present in the growth medium. Tissue culture contamination can be avoided by carrying out drastic disinfection treatments. A profilatic measure would amply to keep the Virology laboratories separated from the rest of the hospital in which Ps. aeruginosa is often present.


Assuntos
Técnicas de Cultura , Pseudomonas aeruginosa , Portador Sadio/epidemiologia , Linhagem Celular , Departamentos Hospitalares , Humanos , Itália , Recursos Humanos em Hospital , Infecções por Pseudomonas/epidemiologia , Virologia
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