[Study of the latex agglutination reaction using a light-scattering method]. / Issledovanie reaktsii lateksnoi aggliutinatsii metodom svetorasseianiia.

Light scattering techniques as a method for monitoring latex agglutination test were under study. The model system, employed in the studies, consisted of a suspension containing polystyrene latex microspheres with tetanus antitoxin immobilized on them (carrier particles) and antitetanus serum (3000 IU). The studies helped define approaches to the choice of the optimal conditions for monitoring the latex agglutination test. Light scattering method has proved to be highly sensitive, its sensitivity essentially surpassing that of slide tests and permitting the detection of early agglutination stages.

[Detection of the intercellular borders of the endothelium using scanning electron microscopy]. / Vyiavlenie mezhkletochnykh granits éndoteliia s pomoshch'iu skaniruiushchei élektronnoi mikroskopii.

Three different approaches for studying aorta endothelial cells by scanning electron microscopy (SEM) were compared to expose cell border lines and surface shape simultaneously. Staining of aorta probes with silver nitrate leads to more specific silver deposits at cell junctions, but unsatisfactory image resolution obscures any detail of cell junctions and other fine structural features. Endothelium impregnated with tannin-osmium showed enhanced cell junction detail, but this method resulted in formation of presumably artificial structures. Osmium tetraoxide was most optimal for impregnation of aorta probes, as it exposed well cell border lines, excluded artifacts of tannin-osmium staining, provided a good image resolution in SEM, and allowed to analyse the ultrastructural features of cell surface.

[Characteristics of the course of abscesses during effects on the system of phagocytosing mononuclear cells]. / Osobennosti techeniia abstsessov v usloviiakh vozdeistviia na sistemu fagotsitiruiushchikh mononuklearov.

In a series of experiments on 230 noninbred white rats the trend in the development of soft tissue abscess was investigated under stimulation and inhibition of phagocytizing mononuclear cells (PMC). The results suggest the stimulation to promote more reliable localization of the inflammation focus and encapsulation with connective tissue. The test for alpha-naphthyl esterase and quantitation of macrophages (absolute number) per infiltration area unit adjacent to the abscess suggest a direct correlation between the absolute number of macrophages and the width of the capsule. The data obtained confirm the assumptions on the role of macrophages in inflammation focus as a regulatory and controlling factor for cellular reactions. PMC system is shown to affect the process of abscess separation from adjacent tissues including formation of the capsule.