RESUMO
Oral squamous cell carcinoma (OSCC) is a prevalent and significant type of oral cancer that has far-reaching health implications worldwide. Epigenetics, a field focused on studying heritable changes in gene expression without modifying DNA sequence, plays a pivotal role in OSCC. Epigenetic changes, encompassing DNA methylation, histone modifications, and miRNAs, exert control over gene activity and cellular characteristics. In OSCC, aberrant DNA methylation of tumor suppressor genes (TSG) leads to their inactivation, subsequently facilitating tumor growth. As a result, distinct patterns of gene methylation hold promise as valuable biomarkers for the detection of OSCC. Oral cancer treatment typically involves surgery, radiation therapy, and chemotherapy, but even with these treatments, cancer cells cannot be effectively targeted and destroyed. Researchers are therefore exploring new methods to target and eliminate cancer cells. One promising approach is the use of epigenetic modifiers, such as DNA methyltransferase (DNMT) inhibitors and histone deacetylase (HDAC) inhibitors, which have been shown to modify abnormal epigenetic patterns in OSCC cells, leading to the reactivation of TSGs and the suppression of oncogenes. As a result, epigenetic-targeted therapies have the potential to directly alter gene expression and minimize side effects. Several studies have explored the efficacy of such therapies in the treatment of OSCC. Although studies have investigated the efficacy of epigenetic therapies, challenges in identifying reliable biomarkers and developing effective combination treatments are acknowledged. Of note, epigenetic mechanisms play a significant role in drug resistance in OSCC and other cancers. Aberrant DNA methylation can silence tumor suppressor genes, while alterations in histone modifications and chromatin remodeling affect gene expression related to drug metabolism and cell survival. Thus, understanding and targeting these epigenetic processes offer potential strategies to overcome drug resistance and improve the efficacy of cancer treatments in OSCC. This comprehensive review focuses on the complex interplay between epigenetic alterations and OSCC cells. This will involve a deep dive into the mechanisms underlying epigenetic modifications and their impact on OSCC, including its initiation, progression, and metastasis. Furthermore, this review will present the role of epigenetics in the treatment and diagnosis of OSCC.
RESUMO
Introduction: Bleach-shade composite resins were recently introduced to the market due to the high demand of patients for whiter teeth. This study sought to compare four stain removal methods for bleach-shade composite resins. Materials and Methods: Seventy-two discs were fabricated from each of the Filtek Z350 XT and Gradia XBW composite resins and immersed in coffee or sour cherry juice staining solutions. Each group was then divided into four subgroups to assess the efficacy of four stain removal methods (finishing with soft-lex disk "brush with pumice" bleaching with carbamide peroxide 16%, bleaching with hydrogen peroxide 40%). The color of each specimen was measured by Easyshade spectrophotometer, and data were analyzed using SPSS 25 statistical package for social sciences. Results: The home-bleaching technique was more effective than the office-bleaching and pumice for the removal of sour cherry juice stain (ΔE = 1.93) and coffee stain (ΔE = 3.32) from Gradia composite discs, almost returning the baseline original color. The Sof-Lex discs were more effective than the pumice for the removal of sour cherry juice stain (ΔE = 4.11) and coffee stain (ΔE = 4.93) from Z350 composite discs but not return the baseline original color. Conclusions: Filtek Z350 had more discoloration than Gradia Direct. The different materials and solutions reacted differently to the four stain removal methods. In GCJ group after all stain removal methods, ΔE reduced to a clinically acceptable level.
