RESUMO
Harnessing the energy of tidal currents has huge potential as a source of clean renewable energy. To do so in a reliable and cost effective way, it is critical to understand the interaction between tidal turbines, waves, and turbulent currents in the ocean. Scaled testing in a tank test provides a controlled, realistic, and highly reproducible down-scaled open ocean environment, and it is a key step in gaining this understanding. Knowledge of the hydrodynamic conditions during tests is critical and measurements at multiple locations are required to accurately characterise spatially varying flow in test tank facilities. The paper presents a laboratory technique using an acoustic velocimetry instrument, the range over-which measurements are acquired being more akin to open water applications. This enables almost simultaneous multi-point measurements of uni-directional velocity along a horizontal profile. Velocity measurements have been obtained from a horizontally mounted Single Beam Acoustic Doppler (SB-ADP) profiler deployed in the FloWave Ocean Energy Research Facility at the University of Edinburgh. These measurements have been statistically compared with point measurements obtained while using a co-located Acoustic Doppler Velocimeter (ADV). Measurements were made with both instruments under flow velocities varying from 0.6 ms-1 to 1.2 ms-1, showing that flow higher than 1 ms-1 was more suitable. Using a SB-ADP has shown the advantage of gaining 54 simultaneous measurement points of uni-directional velocity, covering a significant area with a total distance of 10 m of the test-tank, at a measurement frequency of 16 Hz. Of those measurement points, 41 were compared with co-located ADV measurements covering 8 m of the profile for a tank nominal flow velocity of 0.8 ms-1, and four distributed locations were chosen to to carry out the study at 0.6 ms-1, 1.0 ms-1, and 1.2 ms-1. The comparison with the ADV measurement showed a 2% relative bias on average.
RESUMO
Root proliferation as a response to exploit zones of nutrient enrichment in soil has been demonstrated for a wide range of plant species. However, the effectiveness of this as a strategy to acquire nutrients is also dependent on interactions with the soil microbial community. Specifically, C-flow from roots modifies microbial activity and probably the balance between nutrient mineralization and immobilization processes in the rhizosphere. In this study, near-natural abundance 13C-labelling and gene-reporter methods were applied to determine the effects of uneven nitrate supply to roots of Hordeum vulgare on assimilate partitioning and root exudation. Plants were initially grown in uniform nitrate supply in split-root, sand microcosms after which one treatment continued to receive uniform supply, and the other received nitrate to one root compartment only. At the time of imposing the treatments, the CO2 supplied to the plants was switched to a cylinder source, providing a distinct delta13C-signature and allowing the fate of new assimilate within the plants to be determined. The labelling approach allowed quantification of the expected preferential allocation of new C-assimilate to roots in enriched nitrate, prior to any measurable effect on whole biomass or root architecture. Biosensor (lux-marked Pseudomonas fluorescens 10586 pUCD607) bioluminescence, quantified spatially by CCD imaging, demonstrated that root exudation was significantly increased for roots in enriched nitrate. This response of root exudation, being primarily associated with root apices and concurrent with enhanced assimilate supply, strongly suggests that C-flow from roots is an integral component of the proliferation response to nitrate.
