RESUMO
Sublethal concentration of the antiseptic composition Desoxon-1 was shown to provoke in cells of Corinebacterium ammoniagenes in a liquid medium the biosynthesis and accumulation of a novel macroergic 2-methylbutane-1,2,3,4-tetraol-2,4-cyclopyrophosphate. This substance is also synthesized when C. ammoniagenes is cultivated in a solid agar medium supplemented with benzylviologen. Cells preloaded with the new cyclopyrophosphate maintain its content when treated with 4% phenol, DP-2, Desoxon-1 or boiled and heated in an autoclave. Experiments with Mycobacterium tuberculosis and BCG revealed the ability of these bacteria to grow in a medium supplemented with BV++ possibly due to ability of synthesis of a new cyclopyrophosphate which was shown to correlate with resistance toward redox-cycling drugs. Accumulation of polyphosphates in the control cells of M. tuberculosis was illustrated by 31P-NMR spectroscopy and disappearance of the polyphosphates during cultivation in a BV(++)-supplemented medium. No signal of the new cyclopyrophosphate was yet registered in cells of M. tuberculosis by 31P-NMR.
Assuntos
Anti-Infecciosos Locais/farmacologia , Brevibacterium/efeitos dos fármacos , Corynebacterium/efeitos dos fármacos , Eritritol/análogos & derivados , Brevibacterium/metabolismo , Corynebacterium/metabolismo , Eritritol/farmacologia , Radicais Livres , Espectroscopia de Ressonância Magnética , Mycobacterium bovis/metabolismo , Mycobacterium tuberculosis/efeitos dos fármacos , Mycobacterium tuberculosis/metabolismo , Oxirredução , Ácido Peracético , Superóxidos/metabolismoAssuntos
Desinfetantes/farmacologia , Mycobacterium bovis/efeitos dos fármacos , Mycobacterium phlei/efeitos dos fármacos , Micobactérias não Tuberculosas/efeitos dos fármacos , Ácido Peracético/farmacologia , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/efeitos dos fármacos , DNA Bacteriano/biossíntese , DNA Bacteriano/efeitos dos fármacos , Relação Dose-Resposta a Droga , Substâncias Macromoleculares , Mycobacterium bovis/metabolismo , Mycobacterium bovis/patogenicidade , Mycobacterium phlei/metabolismo , Mycobacterium phlei/patogenicidade , Micobactérias não Tuberculosas/metabolismo , Micobactérias não Tuberculosas/patogenicidade , RNA Bacteriano/biossíntese , RNA Bacteriano/efeitos dos fármacosRESUMO
The changes in the inclusion of 3H-phenylalanine, methyl-3H-thymidine, [2-14C]-thymidine and [5-3H]-uridine in the cells of pathogenic M. bovis-8, opportunistic M. fortuitum and saprophitic M. phlei, M. B-5 were comparatively investigated in introduction of 0.01 and 0.10% concentrations of the drug DP-2. It is shown that 0.10% DP-2 completely stops the radionuclides supply to the cells. It is suggested that the target of the drug may exist in the sphere of amino acid biosynthesis. The presence of DP-2 is capable of disturbing transport of substances to the cells.