Pressmud Subdue Phytoremediation Indices in Lead-Contaminated Soils: A Human Health Perspective.

Direct discharge of waste into water bodies and mining are two major sources of lead contamination in ecosystems. Water scarcity promoted the usage of industrial effluent-contaminated waters for crop production, mainly in peri-urban areas. These wastewaters may contain heavy metals and pollute crop ecosystems. These metals can reach the living cell via contaminated raw foodstuffs that grow under these conditions and cause various ill effects in metabolic activities. In this study, graded levels of pressmud (0, 2.5, 5, 10 g/kg) were applied on lead imposed soil with different contamination levels (0, 100, 150, 300 mg/kg) and metal dynamics was studied in spinach crop. Experimental results showed that the addition of pressmud upto 10 mg/kg had decreased different phytoremediation indices in spinach crop. Whereas, increasing Pb level enhanced the indices' values, indicating accumulation of significant amount of Pb in spinach biomass. However, application of pressmud (upto 10 mg/kg) reduced the bioconcentration factor (BCF) from 0.182 to 0.136, transfer factor (TF) from 0.221 to 0.191, translocation efficiency 66.11-59.34%; whereas, Pb removal enhanced from 0.063 to 0.072 over control treatment. These findings suggest that application of pressmud declined Pb concentration, the BCF and the TF in test crop which lead to less chances of adverse effect in human. These information are very useful for effectively managing wastewater irrigated agricultural crop production systems.

Potassium fractions in black soil mediated by integrated nutrient management modules under maize-chickpea cropping sequence.

A field experiment was conducted at the Research Farm of the ICAR-Indian Institute of Soil Science, Bhopal (India) to study influence of different integrated nutrient management (INM) modules on soil potassium (K) fractions. The experiment comprised with twelve treatments laid out in randomized block design (RBD) with three replications under maize-chickpea cropping sequence. The treatments included general recommended dose (GRD), soil test crop response (STCR) dose; combinations of inorganic and organic inputs and only organic modules. The soil samples were collected at crop harvest and analyzed for various K fractions viz., water soluble-K, available-K, exchangeable-K, HNO3-K, lattice-K and total-K. The results indicated that potassium fractions were significantly (p = 0.05) affected by different treatments. Different INM modules significantly enhanced significantly K availability in soil. Among various INM modules studied, treatment 11 (application of 20 t ha-1 FYM in maize with 5 t ha-1 FYM every year in chickpea) proved most beneficial for improving the soil K fractions. Findings of this type are important for K fertilizer management during crop production in areas with low soil fertility.

Management of plant nutrient dynamics under alkaline soils through graded application of pressmud and gypsum.

An incubation experiment was conducted to monitor the effect of different organic matter inputs with the graded application of gypsum at different time intervals on soil pH, sodium (Na) content and available plant nutrients like nitrogen (N) and sulphur (S) in alkaline soil. The experiment was formulated with nine treatments, i.e. control (T1), recommended dose of fertilizer (RDF) (T2), RDF+Gyp1 (T3), RDF+FYM5+Gyp2 (T4), RDF+FYM10+Gyp1 (T5), RDF+PM5+Gyp2 (T6), RDF+PM10+Gyp1 (T7), RDF+FYM2.5+PM2.5+Gyp2 (T8), RDF+FYM5+PM5+Gyp1 (T9) with three replications. Periodical soil samples were taken at six and twelve months intervals. Results showed that the addition of organic matter reduced the pH and Na content in the soil. More reduction was observed at one year period as compared to six months. The addition of farmyard manure (FYM) and pressmud (PM) at 10 t/ha with gypsum (1 t/ha) improved available N and available S content as compared to organic inputs (5 t/ha) with gypsum (2 t/ha) in soil. Pressmud application with FYM showed better availability of plant nutrients and a reduction of soil pH (8.39 to 7.79) and Na content from 626 to 391 mEq/L in the soil during the incubation period. During the study, the application of treatment T9 (FYM and PM in equal ratio with 1 t/ha gypsum) showed a better availability of available N (175 to 235 kg/ha) and S (15.44 to 23.24 kg/ha) and reduced the active ion concentration of Na. This study is very useful for the management of sodium toxicity, improving soil health and the mineralization rate of organic matter through the application of organic inputs for sustainable crop production.

Management of acid and alkaline phosphatase, dehydrogenase activities by sugarcane industry waste under lead contamination- A case study of Indian Vertisol.

Soil fertility management and crop productivity both are inter-related need extensive attention for sustainability. Industries are being built, which over time produces a lot of effluents containing heavy metal(s), which is then dumped on healthy soils and water bodies. Long-term discharge of lead (Pb)-containing wastewater resulted in significant Pb buildup as well as a decrease in soil biological activity. In this experiment, graded dose of Pb, i.e. 0, 100, 150 and 300 mg/kg and pressmud (PM) (0, 2.5, 5, 10 g/kg) were applied to monitor the Pb toxic effect on soil acid and alkaline phosphatase, dehydrogenase activity. Different treatment combinations were formulated and the experiment was conducted in a completely randomized design (CRD) with three replications. In this experiment, spinach crop was used as a test crop. According to the findings, increased Pb levels in the soil lowered dehydrogenase activity (DHA), acid and alkaline phosphatase. The addition of PM enhanced enzymatic activities by decreasing the labile fraction of Pb in the soil. Incorporation of PM improved the soil enzymatic activities as alkaline phosphatase activity > DHA > acid phosphatase activity in the study. This study suggested that the addition of 10 g/kg PM reduced Pb toxicity (contamination level 300 mg/kg) and improved the soil microbial properties in black soil. These findings are very useful for the remediation of Pb contaminated soil with the help of PM, particularly in peri-urban Pb effluent irrigated areas.

Can Application of Pressmud Mediated Plant Nutrient Dynamics Under Lead Contaminated Soils of Indian Vertisol?

Long- term application of marginal quality water accumulated significant amount of pollutant into the soil. It reduces soil health parameters, and crop yield and their quality. In this regards, graded application of pressmud (PM), i.e. 0, 2.5, 5 and 10 g/kg was applied on lead (Pb) contamination level (0, 100, 150, 300 mg/kg) and evaluated interaction effect on plant nutrients uptake by spinach. Analytical data showed that increasing the PM levels enhanced the macro (phosphorus, potassium, sulphur) and micro-nutrient (zinc, copper, manganese, iron) concentration into the soil. Whereas, increasing the level of Pb significantly (p ≤ 0.05) reduced the P, K and S nutrient concentration and uptake pattern by spinach crop. Increasing PM levels (control to 10 g/kg soil) improved P, K and S by 46.99, 98.96 and 76.79%, respectively in soil. This study is useful to formulate management strategies for minimizing Pb contamination in the food chain by the application of PM mostly in peri-urban areas.

Pressmud overcome lead toxicity by improving spinach biomass in lead-contaminated soils.

Lead (Pb) pollution is a severe problem that primarily affects food chain in developing countries. Continuous use of Pb containing effluent for growing food crops builds up measurable concentration of Pb in soils; and adversely affects the soil properties and crop produce quality. To reduce the Pb metal toxicity in contaminated soil, a pot experiment was conducted with graded doses of pressmud (PM) (0, 2.5, 5.0, and 10.0 g/kg) and Pb (0, 100, 150, and 300 mg/kg soil). Various metal dynamics parameters were computed after spinach crop was harvested. Result showed that higher doses of Pb (300 mg/kg) diminished the spinach root and shoot biomass during the study; whereas, application of PM improved the spinach biomass. However, increasing the pressmud reduced the Pb concentration in shoot from 6.16, 5.99, 4.94, and 3.39 µg/g. Maximum reduction was measured in highest PM applied treatment in shoot (44.92%) and root (57.33%). In this experiment, increasing level of Pb significantly uptake was recorded with higher application rate of Pb (150 and 300 mg/kg). However, elevated doses of PM from control initially enhanced the small chunk of Pb and drastically reduced the shoot Pb uptake (0.060 to 0.049 mg/pot) in maximum level of PM applied treatment. This study is very useful to improve the soil health by immobilizing the labile fraction of Pb by addition of PM in Pb-contaminated soils.

Immobilization of chromium bioavailability through application of organic waste to Indian mustard (Brassica juncea) under chromium-contaminated Indian soils.

Industrialization results in production of large volume of wastewaters, and disposing of them become a serious problem. The wastewaters may have range of heavy metals, which have an impact on soil and plant health. The objective was to evaluate the influence of farm yard manure (FYM) and pressmud (PM) applications on Indian mustard growth and chromium (Cr) uptake in tannery effluent irrigated Cr-contaminated soil. Soil was collected from the tannery effluent irrigated fields (chromium contaminated) of Shekhpura village of Kanpur, India. A pot culture experiment was carried out by growing Indian mustard (Brassica juncea) var. RH 749 on the Cr-contaminated soil with application of different levels and combinations of FYM and PM (at 0, 2.5, and 5 g kg-1 each). Biomass yield, Cr uptake, bioconcentration factor (BCF), transfer factor (TF), transfer efficiency (TE), and Cr removal indices were examined. Higher doses of FYM and PM resulted in reduction of Cr concentrations in shoot (6.60 to 2.50 µg g-1) and root (27.27 to 9.43 µg g-1); and absorption in plant tissues and had improved total dry matter yield (14.56 to 30.94 g pot-1). The use of FYM and PM had a substantial (p ≤ 0.05) impact on phytoremediation parameters like BCF (0.128 to 0.045), TE (59.61 to 64.51%), and Cr removal (0.65 to 0.51%). Combined application of FYM (5 g kg-1) and PM (5 g kg-1) had enhanced the dry matter yield of shoot (12.51 to 26.40 g pot-1) and root (2.05 to 4.54 g pot-1) and reduced the Cr uptake (138.54 to 108.79 mg pot-1) than the individual amendment addition of FYM (138.52 to 135.89 mg pot-1) and PM (126.02 to 130.52 mg pot-1). Combined application of FYM (5 g kg-1) and PM (5 g kg-1) could be beneficial for remediation of Cr-contaminated areas for cultivation of crops.

Reuse of poor-quality water for sustainable crop production in the changing scenario of climate.

The availability of freshwater is limited for agriculture systems across the globe. A fast-growing population demands need to enhance the food grain production from a limited natural resources. Therefore, researchers and policymakers have been emphasized on the production potential of agricultural crops in a sustainable manner. On the challenging side, freshwater bodies are shrinking with the pace of time further limiting crop production. Poor-quality water may be a good alternative for fresh water in water scarce areas. It should not contain toxic pollutants beyond certain critical levels. Unfortunately, such critical limits for different pollutants as well as permissible quality parameters for different wastewater types are lacking or poorly addressed. Marginal quality water and industrial effluent used in crop production should be treated prior to application in crop field. Hence, safe reuse of wastewater for cultivation of food material is necessary to fulfil the demands of growing population across the globe in the changing scenario of climate.

Wastewater irrigation in India: Current status, impacts and response options.

Wastewater generated from urban agglomerations in India is estimated to be 26.4 km3 annually and 28% of it is treated. This has a potential to irrigate about 2.1 million-ha agricultural land, contribute 4 million Mg of plant nutrients, generate 2.8 million person-days of employment and reduce green house gas (GHG) emission by 73.7 million Mg CO2-e. Farmers in peri-urban areas depend largely on raw and partially treated wastewater for livelihood via raising high value crops such as vegetable, fodders and fruits. Both controlled and uncontrolled disposal of waste waters leads to progressive and irreversible contamination of soils, surface and ground waters with pathogens, heavy metals and organic micro-contaminants and consequently their bio-transfer through the chain: sewage-soil-vegetation-animal-humans. This has led to the development of a considerable assortment of regulatory measures and guidelines aimed at reducing or eliminating wastewater related health risks. Because conventional treatment technologies are cost prohibitive, alternate methods based on biological and land treatment systems are being advocated. Since soils are the most logical sinks for wastewater, efforts are to optimise rates and methods of water application, quantify the sink capacity of soils to immobilise contaminants and protect the quality of produce. Reuse of diluted or undiluted wastewaters improves crop productivity by 10-36% though production sustainability depends on soil type, climatic conditions, crop grown, irrigation techniques and socio-political factors. Disposal of wastewater in tree plantations and constructed wetlands with consequent removal of toxic metals/compounds using hyper-accumulators/accumulators plants provide for a possible alternative. Ignoring the associated risks, using pisciculture for sewage disposal is quite popular in high rainfall areas. With growing water scarcities, it is utmost important to recognise wastewaters as a valuable resource and formulate appropriate policy initiatives considering the health and livelihood issues of the per-urban farmers and consumers of food as well as risks to environment.

Maturity indices as an index to evaluate the quality of sulphur enriched municipal solid waste compost using variable byproduct of sulphur.

The aim of this study was to assess the maturity indicators of municipal solid waste compost (MSWC) enrichment with different byproduct of (sugar and fertilizer industry) sulphur (S). The concentration of total S (TS), water-soluble S (WSS), HCl extractable S and available S were significantly different in composts prepared through different byproduct of S with MSW. WSS varied from 4.6 to 5.9% of TS after 120 days of the composting period, whereas, available S varied from 14.5 - 8.6% of TS. S enriched MSW compost had lower C/N, C/S ratio and higher nitrification index as well as lower phyto-toxicity, demonstrating that composts are properly matured and stabilised. Highest compost quality index (0.97) was recorded with S1 compost. Arylsulphatase activity significantly increased with compost maturity. Results stated that all S enriched products maintained a superior amount of plant nutrients and quality indices, indicating that S enriched compost could be a possible substitute for expensive fertilizers.

Impact of arsenic-polluted groundwater on soil and produce quality: a food chain study.

The experiment was conducted to assess the impact of arsenic (As)-contaminated groundwater irrigation on soil health and crop quality. Geo-referenced groundwater, soil, and crop produce samples were collected from the middle Gangetic plains of Maner block of Patna and were analyzed for As content. The result showed that long-term application of As-contaminated groundwater (0.017 to 0.677 mg L-1) buildup significant amount of As in the soil (0.41 to 8.66 mg kg-1). A significant correlation (r2 = 0.922) was also observed between As content in groundwater and the soil. The content of As in groundwater also affected crop quality and accumulated metal content in different crop parts. Total As content in crop samples ranged from 0.010 to 0.963 µg g-1 of dry weight. The average As content in crop followed order: oilseeds > cereals > vegetables > pulses. Therefore, produce quality should be monitored frequently for As uptake as there is a great chance of As accumulation in food crops. Hence, these approaches are useful for the formulation of policy guidelines for the management of As-containing groundwater and routine risk assessment of As-contaminated soils.

Reducing chromium uptake through application of calcium and sodium in spinach.

In peri-urban areas, the use of wastewater for crop production is a common practice due to water scarcity. Moreover, in the recent years, large quantity of wastewater generation and discharge as industrial effluent in water resources is another issue for reduction of water quality. The leather industries are significantly contributing chromium (Cr) in effluent, whereas, other industries may have salt and cationic load in their discharges are mixed up. Therefore, it is mandatory to study the interactive effect of different effluent constituents on crop plants. In this connection, a pot culture experiment was conducted at the ICAR-Indian Institute of Soil Science, Bhopal to compute the effect of application of calcium (Ca) and sodium (Na) ions on Cr uptake by spinach crop in Vertisol of central India. Three levels of Cr (0, 50, 100 mg kg-1), calcium (0, 2, 4 mM), and sodium (0, 40, 80 mM) were applied in combinations. The spinach variety All Green was used as a test crop and harvested at full maturity. Results showed that application of Ca and Na reduced the Cr uptake in spinach crop. The reduction of Cr uptake was more in the root than shoot. Applied calcium acted as an essential plant nutrient and enhanced the crop biomass. Sole applications of Na adversely affected the crop biomass and Cr uptake in both root and shoot of spinach. In conclusion, application of Ca fertilizers reduced the Cr toxicity in spinach and could be used as a strategy for the safe utilization of tannery industrial effluents for crop production.

Chromium toxicity mediated by application of chloride and sulfate ions in Vertisol of Central India.

Chromium (Cr) is one of the toxic metals adversely affecting organisms including humans in the ecosystems, and it is present in considerable concentration in the tannery industrial effluent. Toxicity expression of Cr is suspected to be influenced considerably by other accompanying ions present in the effluent used for irrigation. In a screen house experiment, interactive effects of chloride and sulfate ions in a Vertisol on uptake of Cr by spinach crop were investigated and treatments imposed were three levels each of Cr (0, 50, 100 mg kg-1), chloride (Cl-) (0, 25, 50 mM kg-1), and sulfur (S) (0, 4, 8 mM kg-1) in possible combinations. Plant growth parameters and leaf Cr concentrations were recorded to find out the effect of anions on Cr dynamics in the plant. Increasing the concentration of Cl- ions in soil reduced the Cr concentration in both root and shoot. Similarly, increasing the concentration of S from 4 to 8 mM kg-1 also reduced the concentration and uptake of Cr. Application of sulfate ions augmented the plant growth and counters the negative effect of Cl- ions and Cr. Thus, the study revealed that the addition of S fertilizers could minimize the Cr toxicity in high Cr contaminated soils.

Impact of Long-Term Application of Sewage on Soil and Crop Quality in Vertisols of Central India.

Shortfall of rain and the decreased groundwater level force farmers to use poor quality water for crop production in developing countries. In this study, the quality of agricultural produce and soil health affected by sewage water (Patranala) irrigation was evaluated. Sewage sediment, soil and crop samples were analyzed for physico-chemical properties. Sewage water found to contain trace concentration of heavy metals. However, long-term application of this water for crop production could build up a significant amount of trace metals in both soil and sediments. The DTPA extractable heavy metals ranged Cu 2.7-7.59, Cd 0.04-0.06, Pb 1.29-2.05, Cr 0.01-0.04, Ni 0.24-1.03 and Zn 0.63-2.59 mg kg-1 soil. The heavy metal risk assessment (hazard quotient, HQ) was calculated and found that HQ for these metals in the crops under study was in safe limits. From the study, it is concluded that growing vegetables using sewage water of Patranala is safe, but periodic monitoring to be carried out to avoid food chain contamination.

Soil Enzymatic Activities as Influenced by Lead and Nickel Concentrations in a Vertisol of Central India.

A pot-culture was conducted in Completely Randomized Design with three replicates to study the effect of Pb and Ni on enzymatic activities in a Vertisols. Results indicated that increasing in the levels of Pb from 0, 100, 150 and 300 mg kg-1 soil significantly reduced the dehydrogenase activity (DHA) 38.9, 32.1, 30.9, 18.1 µg triphenylformazan g-1 soil 24 h-1; acid phosphatase activities 73, 61, 58, 55 µg PNP g-1 soil h-1 and alkaline phosphatase activities 80.7, 69.4, 66.2 and 64.0 µg PNP g-1 soil h-1, respectively. Application of Ni up to 100 mg kg-1 had significantly improved the soil enzymatic activities and thereafter there was no such change up to the highest level (300 mg Ni kg-1). Among soil enzymatic activities, DHA was more sensitive to Pb application. The findings generated through this study can be useful for managing waste water for safe disposal as well as sustainable crop production.

Carbon and nitrogen mineralization in Vertisol as mediated by type and placement method of residue.

Selection of appropriate residue application method is essential for better use of biomass for soil and environmental health improvement. A laboratory incubation experiment was conducted for 75 days to investigate C and N mineralization of residues of soybean (Glycine max L.), chickpea (Cicer arietinum L.), maize (Zea mays L.), and wheat (Triticum aestivum L.) placed on the soil surface and incorporated into the soil. The residue of soybean and chickpea had a greater decomposition rate than that of maize and wheat, despite of their placements. Higher rate of decomposition of the residue of soybean and chickpea was recorded when it was kept on the soil surface while soil incorporation of residue of wheat and maize resulted in faster decomposition. Therefore, these findings could be used as guidelines for management of crop residue application in farmland to improve soil and environmental quality.

Geo-Accumulation Indices of Heavy Metals in Soil and Groundwater of Kanpur, India Under Long Term Irrigation of Tannery Effluent.

Soil and groundwater from long-term (>50 years) tannery effluent irrigated areas of Kanpur were analyzed and significant buildup of heavy metals such as Cr, Ni, Cd, Pb, Zn, and As in the range of 252-972, 23-30, 2.3-14.1, 23.7-58.8, 138-338 and 6.8-11 mg kg-1, respectively in soil was found. Few groundwater samples in the effluent irrigated areas also exhibited high Cr concentration above the permissible limit of United States Environmental Protection Agency. The tannery effluents contained 1.53-57.3 ppm Cr, 0-0.12 ppm Ni, 0-0.02 ppm Cd, 0-0.07 ppm Pb, 0-0.48 ppm Zn and 0-0.03 ppm As. The Geo-accumulation index (Igeo) revealed that soil samples were unpolluted to moderately polluted with Cu, Ni, Zn, Pb and As; moderately polluted in case of Cd; and heavily to extremely polluted by Cr.

Impact of pigeon pea biochar on cadmium mobility in soil and transfer rate to leafy vegetable spinach.

Introduction of heavy metals in the environment by various anthropogenic activities has become a potential treat to life. Among the heavy metals, cadmium (Cd) shows relatively high soil mobility and has high phyto-mammalian toxicity. Integration of soil remediation and ecosystem services, such as carbon sequestration in soils through organic amendments, may provide an attractive land management option for contaminated sites. The application of biochar in agriculture has recently received much attention globally due to its associated multiple benefits, particularly, long-term carbon storage in soil. However, the application of biochar from softwood crop residue for heavy metal immobilization, as an alternative to direct field application, has not received much attention. Hence, a pot experiment was conducted to study the effect of pigeon pea biochar on cadmium mobility in a soil-plant system in cadmium-spiked sandy loam soil. The biochar was prepared from pigeon pea stalk through a slow pyrolysis method at 300 °C. The experiment was designed with three levels of Cd (0, 5, and 10 mg Cd kg(-1) soil) and three levels of biochar (0, 2.5, and 5 g kg(-1) soil) using spinach as a test crop. The results indicate that with increasing levels of applied cadmium at 5 and 10 mg kg(-1) soil, the dry matter yield (DMY) of spinach leaf decreased by 9.84 and 18.29 %, respectively. However, application of biochar (at 2.5 and 5 g kg(-1) soil) significantly increased the dry matter yield of spinach leaf by 5.07 and 15.02 %, respectively, and root by 14.0 and 24.0 %, respectively, over the control. Organic carbon content in the post-harvest soil increased to 34.9 and 60.5 % due to the application of biochar 2.5 and 5 g kg(-1) soil, respectively. Further, there was a reduction in the diethylene triamine pentaacetic acid (DTPA)-extractable cadmium in the soil and in transfer coefficient values (soil to plant), as well as its concentrations in spinach leaf and root, indicating that cadmium mobility was decreased due to biochar application. This study shows that pigeon pea biochar has the potential to increase spinach yield and reduce cadmium mobility in contaminated sandy soil.

Assessment of chromium efficacy on germination, root elongation, and coleoptile growth of wheat (Triticum aestivum L.) at different growth periods.

The tannery effluents contain a high concentration of chromium (Cr). It drastically reduces the crop yield when used for irrigation purpose. A huge volume of tannery effluents is available as irrigation for crop production. It is negatively affecting germination as well as yield of the crop. The wheat seeds were exposed to five different concentrations of Cr (0, 20, 40, 80, and 100 ppm). In Petri plates, 100 seeds were placed and the germination percent was recorded after 72 hour (h). Root elongation and coleoptile growth were measured at 72, 120, 168, and 240 h. Results showed that the germination percent of the test crop decreased with increasing Cr levels. It decreased by 6, 14, 30, and 37 % under the Cr concentration of 20, 40, 80, and 100 ppm, respectively. The root elongation was more sensitive than the coleoptile growth. The negative correlation was found between Cr levels and root elongation as well as coleoptile growth. These growth parameters were significantly affected up to 80 ppm of Cr level. The wheat growers using tannery effluent as irrigation should be well treated prior to application.