RESUMO
The property of biomaterials to control the sequential release of growth factors has been widely concerned in the field of bone repair and regeneration. Double-factor sustained-release scaffolds have good biocompatibility and bioactivity. OCarboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation. This study developed scaffolds materials with microspheres with a purpose of forming a controlled slow release secondary structure. O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release. The O-CMCS were loaded with rhBMP-2 and the hydroxyapatite collagen (HC) scaffolds were loaded with VEGF to prepare the scaffolds. These were double factors composite sustained-release system scaffolds. The biocompatibility of the sustained-release system was evaluated by in vitro and in vivo experiments. HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments. The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.
Assuntos
Materiais Biocompatíveis/química , Proteína Morfogenética Óssea 2/administração & dosagem , Regeneração Óssea/efeitos dos fármacos , Engenharia Tecidual , Fator de Crescimento Transformador beta/administração & dosagem , Fator A de Crescimento do Endotélio Vascular/administração & dosagem , Animais , Materiais Biocompatíveis/administração & dosagem , Materiais Biocompatíveis/síntese química , Proteína Morfogenética Óssea 2/química , Proteína Morfogenética Óssea 2/genética , Regeneração Óssea/genética , Osso e Ossos/efeitos dos fármacos , Quitosana/análogos & derivados , Quitosana/química , Colágeno/administração & dosagem , Colágeno/química , Liberação Controlada de Fármacos/efeitos dos fármacos , Durapatita/síntese química , Durapatita/química , Humanos , Microscopia Eletrônica de Varredura , Microesferas , Osteogênese/efeitos dos fármacos , Ratos , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Espectroscopia de Infravermelho com Transformada de Fourier , Alicerces Teciduais/química , Fator de Crescimento Transformador beta/química , Fator de Crescimento Transformador beta/genética , Fator A de Crescimento do Endotélio Vascular/química , Fator A de Crescimento do Endotélio Vascular/genéticaRESUMO
Owing to low bearing capacity and efficiency, traditional filters or adsorbents for removal of contaminants like crystal violet (CV) dye required frequent replacement. Besides, the combination of three-dimensional (3D) printing and bionics could break the constraints of traditional configuration. In this study, a novel depth-type hybrid polylactic acid (PLA)@graphene oxide (GO)/chitosan (CS) sponge filter with bionic fish-mouth structure was prepared and fabricated, assisted by 3D printing and double freeze-drying technology, according to the theories of vertical cross-step filtration and swirling flow. And GO/CS sponge and its filtering device were characterized by FITR, SEM, water adsorption and so on. Moreover, it was explained that the impact factors on dye removal mechanism, like GO content (or CS content), contact time, pH, temperature and bionic configuration. As a result, the bionic 3D filtering device demonstrated excellent removal efficiency (97.8±0.5% for CV) and GO/CS sponge exhibited higher strength (74.5±3.5MPa) at the condition of GO content of 9wt%, contact time of 46min, pH of 8 and 35°C, respectively. Therefore, the resulting 3D PLA@GO/CS sponge bionic filter via gravity and vortex driving provided new alternatives for effectively dye-water separation, and it showed great promise for application of biological macromolecules in adsorption.
