[Effect of Sulfakombin SPOFA on the dynamics of the extent of coccidiosis in small rabbit breeding operations]. / Ucinnost Sulfakombinu SPOFA na dynamiku extenzity kokcidiózy v drobných chovech králíku.

We determined the yearly changes in coccidiosis extensity applying the results of an examination of 2707 rabbits performed in the years 1981-1984. A field trial was conducted to study the efficiency of the Czechoslovak anticoccidic drug Sulfakombin Spofa administered to control coccidiosis in small rabbit breedings. We made the calculations to find out the optimum time of administration of sulphonamide substances. We discuss certain factors that influence in a decisive way the changes in coccidiosis extensity in rabbit breedings.

[Testing the protective effectiveness of vaccines against infection with Pseudomonas aeruginosa in mink (Lutreola vison)]. / Overování protekcní úcinnosti vakcíny proti infekci Pseudomonas aeruginosa u norku (Lutreola vison).

Infections by Pseudomonas aeruginosa have caused losses on mink farms in recent years, particularly with a clinical manifestation of haemorrhagic pneumonia. This paper includes the first results of the practical use of the Czechoslovak soluble monovaccine of polyvalent action in the treatment of mink infected by Pseudomonas aeruginosa. The action of the vaccine is based on the protective effect of Original Endotoxin Protein (OEP), antigen common to all species of the genus Pseudomonas. After due testing, the vaccine was given to mink. Doses of 50, 200 and 500 micrograms, and in another series 50 and 200 micrograms of the vaccine were tested in subcutaneous administration at 0.2 ml volume in a 7-day interval. No adverse side-effects and reactions were observed in the animals. A protective action was demonstrated, resulting in a higher number of reared mink in comparison with the control groups. After vaccination, titres ranging from 2 to 160 were determined by the indirect haemagglutination method and from 320 to 164,000 by the RIA method. In 1984 the vaccine was used for practical treatment on a mink farm with the stock exposed to Pseudomonas aeruginosa infection (5551 mink were treated). In the vaccinated group losses amounted to 3.1% whereas in the control group the mortality level was above 17%. Vaccination was demonstrated to have a favourable effect and the vaccine was then preventively used on the same farm in the subsequent year; the treatment of 29,350 mink had the required protective effect.

[Vaccination of ferrets (Putorius furo) against rabies using a tissue vaccine]. / Vakcinace fretek (Putorius furo) proti vztekline tkánovou vakcínou.

The use of live tissue rabies vaccine from Vnukovo-32 strain produced by the Bioveta National Corporation at Ivanovice na Hané was tested on 15 ferrets (Putorius furo L. 1758). The 21st day after subcutaneous application of 3 ml of vaccine, the average titre of virus-neutralizing antibodies was 1 : 71.4, and after subcutaneous and intramuscular application of 1 ml of vaccine the average titre was 1:20.6 and 1 : 15.6, respectively. The challenge test was performed 52 weeks after vaccination: a dose of 10(5) MICLD50 of the street rabies virus was applied i. m. to the neck muscle. All the vaccinated animals survived. As indicated by the results of the trials, a good tolerance and full immunity of vaccinates to the infection with the street rabies virus was maintained for one year. 1 ml of vaccine diluted according to instructions and applied s. c. or i. m. was found to be sufficient.

[Encephalitozoonosis in farm-bred arctic blue foxes (Alopex lagopus)]. / Encefalitozoonóza ve faremním chovu modrých pescu polárních (Alopex lagopus).

Occurrence of encephalitozoonosis in farm breeding of blue fox is described. Fifty youngs of eight breeding females were infected, mortality was 88%. The main clinical symptoms of the disease were somnolence, ataxia, vision disorders, clonic spasms, at a protracted course also retarded growth. Typical nonpurulent microgranulomata with occurrence of individual spores and cysts of Encephalitozoon cuniculi were demonstrated in liver and central nervous system. Further, diffusion interstitial nephritis and necrotizing angiitis were determined. In breeding females, chronical interstitial nephritis was determined, without detection of the causative agent. A probable source of infection was the feeding of dead rabbits with subsequent transmission to progeny.