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Biochem J ; 138(2): 233-7, 1974 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-4822732

RESUMO

The fate of the known sterol precursor squalene 2,3-oxide was investigated in the free-living nematode Panagrellus redivivus. The nematodes were cultured axenically in the presence of [4-(3)H]squalene 2,3-oxide. Radioactivity was found in the total lipids of the isolated nematodes. Essentially all of the radioactivity encountered in the total lipids was found in the non-saponifiable fraction. The components present in the non-saponifiable fraction were separated and isolated by t.l.c. Three labelled components were identified by a combination of t.l.c., g.l.c. and mass spectroscopy. It is established that P. redivivus has the capacity for biosynthesis of lanosterol. No labelled C(27) sterols could be detected.


Assuntos
Nematoides/metabolismo , Esteróis/biossíntese , Animais , Cromatografia Gasosa , Cromatografia em Camada Fina , Éteres Cíclicos/metabolismo , Lanosterol/biossíntese , Espectrometria de Massas , Esqualeno/metabolismo , Trítio
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